Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   EXA06_RS15555 Genome accession   NZ_CP035822
Coordinates   3175402..3176895 (-) Length   497 a.a.
NCBI ID   WP_001040655.1    Uniprot ID   -
Organism   Escherichia coli strain WS4437A-1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3170402..3181895
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EXA06_RS15530 panM 3170636..3171019 (+) 384 WP_000778768.1 aspartate 1-decarboxylase autocleavage activator PanM -
  EXA06_RS15535 livJ 3171207..3172310 (-) 1104 WP_001021996.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
  EXA06_RS15540 rpoH 3172581..3173435 (-) 855 WP_000130217.1 RNA polymerase sigma factor RpoH -
  EXA06_RS15545 ftsX 3173680..3174738 (-) 1059 WP_001042003.1 permease-like cell division protein FtsX -
  EXA06_RS15550 ftsE 3174731..3175399 (-) 669 WP_000617723.1 cell division ATP-binding protein FtsE -
  EXA06_RS15555 pilA 3175402..3176895 (-) 1494 WP_001040655.1 signal recognition particle-docking protein FtsY Machinery gene
  EXA06_RS15560 rsmD 3177045..3177641 (+) 597 WP_000743193.1 16S rRNA (guanine(966)-N(2))-methyltransferase -
  EXA06_RS15565 yhhL 3177631..3177900 (+) 270 WP_001311191.1 DUF1145 family protein -
  EXA06_RS15570 yhhM 3177903..3178262 (-) 360 WP_000042895.1 DUF2500 domain-containing protein -
  EXA06_RS15575 yhhN 3178403..3179029 (+) 627 WP_000964718.1 lysoplasmalogenase -
  EXA06_RS15580 zntA 3179103..3181301 (+) 2199 WP_000106551.1 Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase ZntA -
  EXA06_RS15585 tusA 3181403..3181648 (-) 246 WP_000130621.1 sulfurtransferase TusA -

Sequence

Protein


Download         Length: 497 a.a.        Molecular weight: 54528.32 Da        Isoelectric Point: 4.1560

>NTDB_id=305318 EXA06_RS15555 WP_001040655.1 3175402..3176895(-) (pilA) [Escherichia coli strain WS4437A-1]
MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEPVKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVAE
SEKAQPEAEVVAQPEPVVEETPEPVAIEREELPLPEDVNAEAVSPEEWQAEAETVEIVEAAEEEAAKEEITDEELETALA
AEAAEEAVMVVPPAEEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISLFRGKKIDDDLFEELEEQLLIADV
GVETTRKIITNLTEGASRKQLRDAEALYGQLKEEMGEILAKVDEPLNVEGKAPFVILMVGVNGVGKTTTIGKLARQFEQQ
GKSVMLAAGDTFRAAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKSHLMEELKKI
VRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLDGTAKGGVIFSVADQFGIPIRYIGVGERIEDL
RPFKADDFIEALFARED

Nucleotide


Download         Length: 1494 bp        

>NTDB_id=305318 EXA06_RS15555 WP_001040655.1 3175402..3176895(-) (pilA) [Escherichia coli strain WS4437A-1]
ATGGCGAAAGAAAAAAAACGTGGCTTTTTTTCCTGGCTGGGCTTTGGTCAAAAAGAGCAGACCCCGGAAAAAGAGACAGA
AGTTCAGAATGAACAACCGGTTGTAGAAGAAATCGTTCAGGCGCAAGAGCCTGTGAAGGCCTCTGAACAAGCCGTTGAAG
AGCAGCCGCAAGCGCATACTGAAGCCGAGGCGGAAACTTTTGCTGCCGACGTTGTGGAAGTCACTGAACAGGTTGCTGAA
AGTGAAAAAGCGCAGCCTGAAGCGGAAGTCGTTGCACAGCCGGAACCGGTCGTAGAAGAAACGCCGGAGCCAGTGGCTAT
CGAACGTGAAGAGCTGCCGTTGCCGGAAGACGTCAACGCCGAAGCGGTTTCGCCAGAAGAGTGGCAGGCTGAAGCGGAAA
CCGTAGAGATTGTCGAAGCGGCGGAAGAAGAAGCGGCTAAAGAAGAAATTACCGACGAAGAGCTGGAAACGGCGCTGGCT
GCCGAAGCGGCAGAAGAGGCGGTGATGGTGGTTCCTCCGGCAGAAGAAGAGCAGCCGGTGGAAGAAATCGCTCAGGAGCA
GGAAAAACCGACCAAAGAAGGTTTTTTCGCGCGCCTGAAACGCAGCCTGTTAAAAACCAAAGAAAATCTCGGTTCCGGAT
TTATCAGCCTGTTCCGCGGTAAAAAAATCGACGATGATCTGTTTGAGGAGCTGGAAGAGCAGCTTTTGATCGCCGATGTG
GGTGTGGAAACCACACGTAAAATTATCACCAATCTGACGGAAGGCGCATCCCGCAAGCAGCTTCGTGACGCCGAGGCGCT
CTATGGCCAGCTGAAAGAAGAGATGGGCGAGATTCTGGCGAAAGTCGATGAGCCGCTGAATGTTGAAGGCAAAGCGCCGT
TTGTGATCCTGATGGTGGGCGTCAACGGTGTGGGTAAAACCACGACGATTGGTAAGCTGGCGCGTCAGTTTGAGCAGCAG
GGTAAATCGGTGATGCTGGCGGCGGGTGATACTTTCCGTGCAGCTGCGGTTGAACAGCTTCAGGTCTGGGGTCAGCGCAA
CAATATTCCGGTGATTGCCCAGCATACCGGGGCGGATTCCGCCTCTGTTATCTTCGACGCCATTCAGGCAGCTAAAGCGC
GTAATATCGACGTCCTGATTGCCGATACAGCCGGACGCCTGCAGAACAAATCGCACCTGATGGAAGAGTTGAAGAAAATC
GTCCGCGTGATGAAGAAACTCGACGTTGAAGCGCCGCATGAAGTTATGCTGACTATTGATGCCAGCACCGGGCAGAACGC
GGTAAGCCAGGCCAAACTGTTCCATGAAGCCGTTGGCTTAACCGGCATCACGCTAACGAAACTGGACGGCACGGCGAAAG
GCGGGGTAATTTTCTCGGTGGCTGACCAGTTTGGTATCCCTATCCGCTACATTGGTGTCGGCGAACGTATTGAGGATTTG
CGTCCGTTTAAGGCGGACGACTTTATAGAGGCACTTTTTGCCCGAGAGGATTAA

Domains

Predicted by InterProScan.

SRP54-type protein, GTPase domain (SRP54)

(293-493)

SRP54-type protein, helical bundle domain (SRP54_N)

(210-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Neisseria gonorrhoeae MS11

48.698

77.264

0.376