Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   DWB91_RS10395 Genome accession   NZ_CP031266
Coordinates   2125373..2126743 (-) Length   456 a.a.
NCBI ID   WP_037566299.1    Uniprot ID   A0A2T4MFZ0
Organism   Staphylococcus agnetis strain 12B     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2120373..2131743
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DWB91_RS10370 (DWB91_10390) cysE 2120631..2121257 (-) 627 WP_168429109.1 serine O-acetyltransferase -
  DWB91_RS10375 (DWB91_10395) gltX 2121587..2123041 (-) 1455 WP_060552323.1 glutamate--tRNA ligase -
  DWB91_RS10380 (DWB91_10400) ispF 2123130..2123606 (-) 477 WP_060552322.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -
  DWB91_RS10385 (DWB91_10405) ispD 2123599..2124294 (-) 696 WP_060552321.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  DWB91_RS10390 (DWB91_10410) - 2124297..2125358 (-) 1062 WP_060552320.1 PIN/TRAM domain-containing protein -
  DWB91_RS10395 (DWB91_10415) radA 2125373..2126743 (-) 1371 WP_037566299.1 DNA repair protein RadA Machinery gene
  DWB91_RS10400 (DWB91_10420) - 2126945..2129407 (-) 2463 WP_107368324.1 ATP-dependent Clp protease ATP-binding subunit -
  DWB91_RS10405 (DWB91_10425) - 2129420..2130424 (-) 1005 WP_060552318.1 protein arginine kinase -
  DWB91_RS10410 (DWB91_10430) - 2130417..2130935 (-) 519 WP_060552317.1 UvrB/UvrC motif-containing protein -
  DWB91_RS10415 (DWB91_10435) - 2130947..2131408 (-) 462 WP_037566295.1 CtsR family transcriptional regulator -

Sequence

Protein


Download         Length: 456 a.a.        Molecular weight: 50082.67 Da        Isoelectric Point: 8.0159

>NTDB_id=305271 DWB91_RS10395 WP_037566299.1 2125373..2126743(-) (radA) [Staphylococcus agnetis strain 12B]
MAKKKITFECTACGYQSPKWMGKCPNCGAWNSMEESLEQKTAHPKHGVRAQKTHAKVQTLNEIKNEQTPRILTKSEEFNR
VLGGGIVQGSLVLIGGDPGIGKSTLLLQMCAALSKTKKVLYITGEESLNQTKLRADRLDEDASALNVFAETDLEVIHDAV
KKVEPELIVVDSIQTIYHPEISSAPGSVSQVRESTQSLMHIAKQMNIATFIVGHVTKEGQIAGPRLLEHMVDTVLYFEGD
AHHAYRILRAVKNRFGSTNEMGIFEMKQSGLKSVPNPSEMFLEERSTNVPGSTIVATMEGTRPLLIEVQALVTPTTFNNP
RRMATGIDHNRLSLLMAVLEKKEGYLLQQQDAYIKVAGGVKLSEPAVDLGIIIATASSFKDQAVDGLDCFIGEVGLTGEV
RRVSRIEQRVQEAAKLGFKRVIIPKTNIGGWTFPENIEVIGVSSTNEALKHALKQR

Nucleotide


Download         Length: 1371 bp        

>NTDB_id=305271 DWB91_RS10395 WP_037566299.1 2125373..2126743(-) (radA) [Staphylococcus agnetis strain 12B]
GTGGCCAAAAAAAAGATTACATTTGAGTGTACTGCATGCGGATACCAGTCTCCAAAATGGATGGGGAAATGCCCAAATTG
CGGTGCATGGAATTCAATGGAAGAATCATTGGAACAAAAAACAGCTCACCCTAAACATGGCGTGCGTGCGCAAAAAACAC
ATGCAAAAGTGCAAACATTAAATGAAATTAAAAATGAACAGACACCACGTATTCTTACTAAAAGTGAAGAATTTAACCGT
GTGCTTGGAGGCGGAATTGTTCAAGGTTCTCTCGTGCTCATCGGGGGCGATCCAGGTATCGGTAAATCAACACTCCTCCT
TCAAATGTGTGCTGCGTTATCTAAAACTAAGAAAGTACTTTATATTACCGGTGAAGAATCGCTAAATCAAACGAAACTAC
GCGCCGACCGTTTAGATGAAGATGCTAGTGCACTCAATGTGTTTGCAGAAACGGATCTTGAAGTCATTCATGATGCAGTA
AAAAAAGTGGAGCCTGAGTTAATCGTTGTAGATTCCATTCAAACCATTTATCACCCAGAAATCAGTTCTGCACCGGGGTC
AGTATCTCAAGTGAGGGAGAGTACACAAAGCCTCATGCATATTGCCAAACAAATGAATATCGCTACATTTATCGTTGGAC
ACGTTACGAAAGAAGGCCAAATTGCGGGTCCTCGATTGTTAGAACATATGGTGGATACTGTGCTCTATTTTGAAGGCGAT
GCACATCATGCATATCGCATTTTGCGTGCGGTGAAGAACCGGTTTGGTTCTACAAATGAAATGGGAATATTTGAAATGAA
ACAATCAGGTTTAAAAAGCGTGCCGAATCCGTCTGAAATGTTTTTAGAAGAACGCTCTACAAATGTACCAGGTTCTACGA
TTGTGGCAACAATGGAAGGAACCCGTCCGTTACTGATTGAAGTGCAAGCTTTAGTCACACCAACAACTTTTAACAATCCT
AGACGAATGGCTACAGGTATAGATCATAATCGCCTAAGTTTGTTAATGGCTGTTTTAGAGAAAAAAGAAGGGTATTTACT
CCAGCAACAAGATGCATATATTAAAGTAGCAGGTGGTGTCAAATTAAGTGAACCCGCTGTTGATTTAGGTATCATTATTG
CGACAGCTTCGAGTTTTAAAGATCAAGCTGTTGATGGATTAGATTGTTTTATAGGTGAAGTAGGTTTAACCGGGGAAGTG
CGCAGAGTCTCCCGTATTGAACAAAGGGTTCAAGAAGCGGCGAAGTTGGGATTCAAACGCGTCATTATTCCGAAAACCAA
TATCGGTGGGTGGACATTCCCAGAAAATATAGAAGTAATCGGTGTGTCTTCAACAAATGAAGCATTAAAGCATGCCTTAA
AACAAAGATAA

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(344-427)

AAA domain (AAA_25)

(62-219)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2T4MFZ0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

66.304

100

0.669
  radA Streptococcus pneumoniae Rx1

59.471

99.561

0.592
  radA Streptococcus pneumoniae D39

59.471

99.561

0.592
  radA Streptococcus pneumoniae R6

59.471

99.561

0.592
  radA Streptococcus pneumoniae TIGR4

59.471

99.561

0.592
  radA Streptococcus mitis NCTC 12261

59.471

99.561

0.592
  radA Streptococcus mitis SK321

59.471

99.561

0.592

Multiple sequence alignment