Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoS   Type   Regulator
Locus tag   EW648_RS17375 Genome accession   NZ_CP035772
Coordinates   3478667..3479659 (-) Length   330 a.a.
NCBI ID   WP_000081550.1    Uniprot ID   A0A370V765
Organism   Escherichia coli O145 strain RM8843-C1     
Function   regulation of chitinases (predicted from homology)   
Competence regulation

Genomic Context

Location: 3473667..3484659
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EW648_RS17350 (EW648_17830) pphB 3475030..3475686 (+) 657 WP_001141343.1 protein-serine/threonine phosphatase -
  EW648_RS17355 (EW648_17835) - 3475727..3475963 (-) 237 WP_000562979.1 non-oxidative hydroxyarylic acid decarboxylases subunit D -
  EW648_RS17360 (EW648_17840) - 3475974..3477401 (-) 1428 Protein_3445 non-oxidative hydroxyarylic acid decarboxylases subunit C -
  EW648_RS17365 (EW648_17845) - 3477401..3477994 (-) 594 WP_000767721.1 non-oxidative hydroxyarylic acid decarboxylases subunit B -
  EW648_RS17370 (EW648_17850) - 3478140..3478547 (+) 408 WP_001208062.1 MarR family transcriptional regulator -
  EW648_RS17375 (EW648_17855) rpoS 3478667..3479659 (-) 993 WP_000081550.1 RNA polymerase sigma factor RpoS Regulator
  EW648_RS17380 (EW648_17860) nlpD 3479722..3480816 (-) 1095 WP_306174120.1 murein hydrolase activator NlpD -
  EW648_RS17385 (EW648_17865) pcm 3481001..3481627 (-) 627 WP_000254708.1 protein-L-isoaspartate O-methyltransferase -
  EW648_RS17390 (EW648_17870) surE 3481621..3482382 (-) 762 WP_001295182.1 5'/3'-nucleotidase SurE -
  EW648_RS17395 (EW648_17875) truD 3482363..3483412 (-) 1050 WP_000568924.1 tRNA pseudouridine(13) synthase TruD -
  EW648_RS17400 (EW648_17880) ispF 3483409..3483888 (-) 480 WP_001219242.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -
  EW648_RS17405 (EW648_17885) ispD 3483888..3484598 (-) 711 WP_000246160.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -

Sequence

Protein


Download         Length: 330 a.a.        Molecular weight: 37972.84 Da        Isoelectric Point: 4.5597

>NTDB_id=304902 EW648_RS17375 WP_000081550.1 3478667..3479659(-) (rpoS) [Escherichia coli O145 strain RM8843-C1]
MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEELLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFA
RRALRGDVASRRRMIESNLRLVVKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNERITSVDTPLGGDSEKALLDILAD
EKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLARRFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQ
GLNIEALFRE

Nucleotide


Download         Length: 993 bp        

>NTDB_id=304902 EW648_RS17375 WP_000081550.1 3478667..3479659(-) (rpoS) [Escherichia coli O145 strain RM8843-C1]
ATGAGTCAGAATACGCTGAAAGTTCATGATTTAAATGAAGATGCGGAATTTGATGAGAACGGAGTTGAGGTTTTTGACGA
AAAGGCCTTAGTAGAAGAGGAACCCAGTGATAACGATTTGGCCGAAGAGGAACTGTTATCGCAGGGAGCCACACAGCGTG
TGTTGGACGCGACTCAGCTTTACCTTGGTGAGATTGGTTATTCACCACTGTTAACGGCCGAAGAAGAAGTTTATTTTGCG
CGTCGCGCACTGCGTGGAGATGTCGCCTCTCGCCGCCGGATGATCGAGAGTAACTTGCGTCTGGTGGTAAAAATTGCCCG
CCGTTATGGCAATCGTGGTCTGGCGTTGCTGGACCTTATCGAAGAGGGCAACCTGGGGCTGATCCGCGCGGTAGAGAAGT
TTGACCCGGAACGTGGTTTCCGCTTCTCAACATACGCAACCTGGTGGATTCGCCAGACGATTGAACGGGCGATTATGAAC
CAAACCCGTACTATTCGTTTGCCGATTCACATCGTAAAGGAGCTGAACGTTTACCTGCGAACCGCACGTGAGTTGTCCCA
TAAGCTGGACCATGAACCAAGTGCGGAAGAGATCGCAGAGCAACTGGATAAGCCAGTTGATGACGTCAGCCGTATGCTTC
GTCTTAACGAGCGCATTACCTCGGTAGACACCCCGCTGGGTGGTGATTCCGAAAAAGCGTTGCTGGACATCCTGGCCGAT
GAAAAAGAGAACGGTCCGGAAGATACCACGCAAGATGACGATATGAAGCAGAGCATCGTCAAATGGCTGTTCGAGCTGAA
CGCCAAACAGCGTGAAGTGCTGGCACGTCGATTCGGTTTGCTGGGGTACGAAGCGGCAACACTGGAAGATGTAGGTCGTG
AAATTGGCCTCACCCGTGAACGTGTTCGCCAGATTCAGGTTGAAGGCCTGCGCCGTTTGCGCGAAATCCTGCAAACGCAG
GGGCTGAATATCGAAGCGCTGTTCCGCGAATAA

Domains

Predicted by InterProScan.

Sigma-70 region 3 (Sigma70_r3)

(174-248)

Sigma-70 region 2 (Sigma70_r2)

(94-163)

Sigma-70 factor, region 1.2 (Sigma70_r1_2)

(56-88)

Sigma-70, region 4 (Sigma70_r4)

(262-315)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A370V765

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoS Vibrio cholerae O1 biovar El Tor strain E7946

73.065

97.879

0.715

Multiple sequence alignment