Detailed information    

insolico Bioinformatically predicted

Overview

Name   sbcB   Type   Machinery gene
Locus tag   EW646_RS13290 Genome accession   NZ_CP035770
Coordinates   2623709..2625136 (+) Length   475 a.a.
NCBI ID   WP_000980539.1    Uniprot ID   -
Organism   Escherichia coli O145 strain RM8988-C1     
Function   promote homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2618709..2630136
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EW646_RS27940 (EW646_13690) - 2619550..2619675 (-) 126 WP_352562283.1 transposase domain-containing protein -
  EW646_RS13265 (EW646_13695) - 2619702..2619884 (+) 183 WP_001016348.1 EutP/PduV family microcompartment system protein -
  EW646_RS13270 (EW646_13700) tmaR 2619985..2620314 (-) 330 WP_000450409.1 DUF496 family protein -
  EW646_RS13275 (EW646_13705) yeeA 2620486..2621544 (-) 1059 WP_001200905.1 FUSC family protein -
  EW646_RS13280 (EW646_13710) sbmC 2621742..2622215 (-) 474 WP_001105393.1 DNA gyrase inhibitor SbmC -
  EW646_RS13285 (EW646_13715) dacD 2622334..2623500 (-) 1167 WP_001320295.1 serine-type D-Ala-D-Ala carboxypeptidase DacD -
  EW646_RS13290 (EW646_13720) sbcB 2623709..2625136 (+) 1428 WP_000980539.1 exodeoxyribonuclease I Machinery gene
  EW646_RS13295 (EW646_13725) tsuB 2625179..2625406 (-) 228 WP_000985273.1 thiosulfate utilization sulfurtransferase TsuB/YeeD -
  EW646_RS13300 (EW646_13730) tsuA 2625420..2626478 (-) 1059 WP_000492332.1 thiosulfate utilization transporter TsuA/YeeE -
  EW646_RS13305 (EW646_13735) plaP 2626657..2628015 (-) 1359 WP_000019197.1 putrescine/proton symporter PlaP -
  EW646_RS13310 (EW646_13740) yoeI 2628005..2628067 (-) 63 WP_010723108.1 membrane protein YoeI -
  EW646_RS13315 (EW646_13745) yeeY 2628282..2629211 (-) 930 WP_000803351.1 LysR substrate-binding domain-containing protein -
  EW646_RS13320 (EW646_13750) yeeZ 2629257..2630081 (-) 825 WP_000754755.1 SDR family oxidoreductase -

Sequence

Protein


Download         Length: 475 a.a.        Molecular weight: 54498.80 Da        Isoelectric Point: 5.3498

>NTDB_id=304812 EW646_RS13290 WP_000980539.1 2623709..2625136(+) (sbcB) [Escherichia coli O145 strain RM8988-C1]
MMNDGKQQSTFLFHDYETFGTHPALDRPAQFAAIRTDDEFNVIGEPEVFYCKPADDYLPQPGAVLITGITPQEARAKGEN
EAAFAARIHSLFTVPKTCILGYNNVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDG
LPSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLMALIDVPQMKPLVHVSGMFGAWR
GNTSWVAPLAWHPENRNAVIMVDLAGDISPLLELDSDTLRERLYTAKADLGDNAAVPVKLVHINKCPVLAQANTLRPEDA
DRLGINRQHCLDNLKILRENPQVREKVVAIFVEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLPALDITFVD
KRIEKLLFNYRARNFPGTLDYAEQQRWLEHRRQVFTPEFLQGYAEEIQMLAQQYADDKEKVALLKALWQYAEEIV

Nucleotide


Download         Length: 1428 bp        

>NTDB_id=304812 EW646_RS13290 WP_000980539.1 2623709..2625136(+) (sbcB) [Escherichia coli O145 strain RM8988-C1]
ATGATGAATGACGGTAAGCAACAATCTACCTTTTTGTTTCACGATTACGAAACCTTTGGCACGCACCCCGCGTTAGATCG
CCCTGCACAGTTCGCAGCCATTCGCACCGATGACGAATTCAATGTCATCGGCGAACCCGAAGTCTTTTACTGCAAGCCCG
CGGATGACTATTTACCCCAGCCTGGAGCAGTATTAATTACCGGTATTACCCCGCAGGAGGCACGAGCGAAAGGAGAAAAC
GAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTACCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTT
CGACGACGAAGTCACACGCAACGTTTTTTATCGTAATTTCTACGATCCTTACGCCTGGAGCTGGCAGCATGATAACTCGC
GCTGGGATTTACTGGATGTTATGCGTGCCTGTTATGCCCTGCGCCCGGAAGGAATAAATTGGCCTGAAAATGATGACGGT
CTACCGAGCTTTCGCCTTGAGCATTTAACCAAAGCGAATGGTATTGAACATAGCAACGCCCACGATGCGATGGCTGATGT
GTACGCCACTATTGCGATGGCAAAACTGGTAAAAACGCGTCAGCCACGCCTGTTTGATTATCTCTTTACCCATCGTAATA
AACACAAACTAATGGCGTTGATTGATGTTCCGCAGATGAAACCCCTGGTTCACGTTTCCGGAATGTTTGGGGCATGGCGC
GGCAATACAAGCTGGGTGGCACCGCTGGCGTGGCATCCTGAAAATCGCAATGCCGTAATTATGGTGGATTTGGCAGGAGA
CATTTCGCCATTACTGGAGCTGGATAGCGACACATTGCGCGAGCGTTTATATACCGCAAAAGCCGATCTTGGCGATAACG
CCGCCGTTCCGGTTAAGCTGGTGCATATCAATAAATGTCCGGTACTGGCCCAGGCGAATACGCTACGCCCGGAAGATGCC
GACCGACTGGGAATTAATCGTCAGCATTGCCTCGATAACCTAAAAATTCTGCGTGAAAATCCGCAAGTGCGTGAAAAAGT
GGTAGCGATATTCGTGGAAGCCGAACCGTTTACACCTTCAGATAATGTGGATGCACAGCTTTATAACGGCTTTTTCAGTG
ACGCCGATCGTGCAGCAATGAAAATTGTGTTGGAAACCGAGCCGCGTAATTTACCGGCACTGGATATCACTTTTGTTGAT
AAACGAATTGAAAAGCTGTTGTTCAATTATCGGGCGCGCAACTTCCCGGGGACGCTGGATTATGCCGAGCAGCAACGCTG
GCTGGAGCACCGCCGCCAGGTCTTCACGCCAGAGTTTTTACAAGGCTATGCAGAAGAGATTCAGATGCTGGCGCAGCAGT
ATGCCGACGATAAAGAAAAAGTGGCGCTGTTAAAAGCGCTTTGGCAGTACGCGGAAGAGATTGTCTAA

Domains

Predicted by InterProScan.

Exonuclease (RNase_T)

(13-188)

Exonuclease C-terminal (ExoI_SH3)

(212-472)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  sbcB Vibrio cholerae O1 biovar El Tor strain E7946

58.723

98.947

0.581

Multiple sequence alignment