Detailed information    

insolico Bioinformatically predicted

Overview

Name   ruvB   Type   Machinery gene
Locus tag   EW649_RS08335 Genome accession   NZ_CP035768
Coordinates   1714563..1715573 (-) Length   336 a.a.
NCBI ID   WP_000568519.1    Uniprot ID   Q32HA1
Organism   Escherichia coli O145 strain RM11626-C1     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1709563..1720573
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EW649_RS08315 (EW649_08505) mepM 1710431..1711753 (-) 1323 WP_001184045.1 murein DD-endopeptidase MepM -
  EW649_RS08320 (EW649_08510) znuA 1711769..1712701 (-) 933 WP_001342995.1 zinc ABC transporter substrate-binding protein ZnuA -
  EW649_RS08325 (EW649_08515) znuC 1712780..1713535 (+) 756 WP_000202996.1 zinc ABC transporter ATP-binding protein ZnuC -
  EW649_RS08330 (EW649_08520) znuB 1713532..1714317 (+) 786 WP_000571478.1 zinc ABC transporter permease subunit ZnuB -
  EW649_RS08335 (EW649_08525) ruvB 1714563..1715573 (-) 1011 WP_000568519.1 Holliday junction branch migration DNA helicase RuvB Machinery gene
  EW649_RS08340 (EW649_08530) ruvA 1715582..1716193 (-) 612 WP_000580323.1 Holliday junction branch migration protein RuvA -
  EW649_RS08345 (EW649_08535) yobI 1716332..1716397 (-) 66 WP_072095795.1 stress response small protein YobI -
  EW649_RS08350 (EW649_08540) yebB 1716468..1717070 (+) 603 WP_001024929.1 YebB family permuted papain-like enzyme -
  EW649_RS08355 (EW649_08545) ruvC 1717072..1717593 (-) 522 WP_001295503.1 crossover junction endodeoxyribonuclease RuvC -
  EW649_RS08360 (EW649_08550) yebC 1717628..1718368 (-) 741 WP_000907248.1 YebC/PmpR family DNA-binding transcriptional regulator -
  EW649_RS08365 (EW649_08555) nudB 1718397..1718849 (-) 453 WP_032161923.1 dihydroneopterin triphosphate diphosphatase -

Sequence

Protein


Download         Length: 336 a.a.        Molecular weight: 37173.77 Da        Isoelectric Point: 4.7818

>NTDB_id=304725 EW649_RS08335 WP_000568519.1 1714563..1715573(-) (ruvB) [Escherichia coli O145 strain RM11626-C1]
MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIFIKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMG
VNLRTTSGPVLEKAGDLAAMLTNLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARGTPRIANRLLRRVRDFAEVKHDG
TISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKFFGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMA
TTRAWNHFGITPPEMP

Nucleotide


Download         Length: 1011 bp        

>NTDB_id=304725 EW649_RS08335 WP_000568519.1 1714563..1715573(-) (ruvB) [Escherichia coli O145 strain RM11626-C1]
ATGATTGAAGCAGACCGTCTGATTTCTGCCGGTACCACTTTGCCGGAAGATGTAGCAGATCGCGCCATTCGCCCTAAATT
ACTGGAAGAGTATGTTGGTCAGCCGCAGGTTCGTTCACAGATGGAGATTTTCATCAAAGCAGCGAAACTGCGCGGCGATG
CCCTCGATCATTTGTTGATTTTTGGTCCTCCGGGGTTGGGTAAAACTACGCTTGCCAACATTGTCGCCAATGAAATGGGC
GTTAATTTACGCACGACTTCTGGTCCGGTGCTGGAAAAGGCGGGCGATTTGGCTGCGATGCTCACTAACCTTGAACCGCA
TGACGTGCTGTTTATTGATGAGATCCACCGTTTATCGCCAGTTGTTGAAGAAGTGCTGTACCCGGCAATGGAAGACTACC
AACTGGATATCATGATTGGTGAAGGTCCGGCGGCACGCTCCATTAAAATTGATTTGCCGCCGTTTACCCTGATTGGTGCA
ACCACGCGCGCAGGTTCGCTGACATCACCGTTGCGCGACCGTTTTGGTATTGTGCAACGTCTGGAGTTTTATCAGGTGCC
GGATCTGCAATATATCGTCAGTCGCAGCGCACGCTTTATGGGGCTTGAGATGAGTGATGACGGCGCGCTGGAAGTTGCTC
GTCGCGCTCGCGGTACGCCGCGCATTGCCAACCGTCTGCTGCGTCGAGTGCGTGATTTCGCCGAAGTGAAGCACGATGGC
ACCATCTCGGCAGATATCGCTGCTCAGGCGCTGGATATGTTGAATGTCGATGCTGAAGGTTTCGATTATATGGACCGCAA
ATTGTTGCTGGCGGTAATCGATAAGTTCTTTGGTGGGCCGGTAGGTCTGGATAACTTGGCGGCAGCCATTGGCGAAGAAC
GTGAAACCATTGAGGATGTGCTGGAACCTTATTTGATTCAGCAAGGCTTTTTGCAGCGTACACCGCGTGGGCGTATGGCG
ACGACGCGGGCGTGGAATCACTTTGGCATAACGCCGCCAGAAATGCCGTAA

Domains

Predicted by InterProScan.

Holliday junction DNA helicase RuvB P-loop domain (RuvB_N)

(23-181)

RuvB AAA lid domain (AAA_lid_4)

(184-257)

RuvB C-terminal winged helix domain (WHD_RuvB)

(259-328)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q32HA1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ruvB Bacillus subtilis subsp. subtilis str. 168

60.486

97.917

0.592
  ruvB Streptococcus pneumoniae TIGR4

58.934

94.94

0.56
  ruvB Streptococcus pneumoniae R6

58.934

94.94

0.56
  ruvB Streptococcus pneumoniae D39

58.934

94.94

0.56
  ruvB Synechocystis sp. PCC 6803

53.251

96.131

0.512
  ruvB Helicobacter pylori 26695

52.038

94.94

0.494

Multiple sequence alignment