Detailed information    

insolico Bioinformatically predicted

Overview

Name   sbcB   Type   Machinery gene
Locus tag   EW647_RS13775 Genome accession   NZ_CP035767
Coordinates   2630369..2631796 (+) Length   475 a.a.
NCBI ID   WP_000980539.1    Uniprot ID   -
Organism   Escherichia coli O145 strain RM12522-C8     
Function   promote homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2625369..2636796
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EW647_RS13735 (EW647_14255) yeeW 2625553..2625747 (+) 195 WP_000988600.1 DUF5983 family protein -
  EW647_RS13740 (EW647_14260) - 2625793..2625873 (+) 81 Protein_2646 hypothetical protein -
  EW647_RS27660 (EW647_14265) - 2626162..2626335 (-) 174 WP_032082634.1 transposase domain-containing protein -
  EW647_RS13750 (EW647_14270) - 2626362..2626544 (+) 183 WP_001016348.1 EutP/PduV family microcompartment system protein -
  EW647_RS13755 (EW647_14275) tmaR 2626645..2626974 (-) 330 WP_000450409.1 DUF496 family protein -
  EW647_RS13760 (EW647_14280) yeeA 2627146..2628204 (-) 1059 WP_001200905.1 FUSC family protein -
  EW647_RS13765 (EW647_14285) sbmC 2628402..2628875 (-) 474 WP_001105393.1 DNA gyrase inhibitor SbmC -
  EW647_RS13770 (EW647_14290) dacD 2628994..2630160 (-) 1167 WP_001320295.1 serine-type D-Ala-D-Ala carboxypeptidase DacD -
  EW647_RS13775 (EW647_14295) sbcB 2630369..2631796 (+) 1428 WP_000980539.1 exodeoxyribonuclease I Machinery gene
  EW647_RS13780 (EW647_14300) tsuB 2631839..2632066 (-) 228 WP_000985273.1 thiosulfate utilization sulfurtransferase TsuB/YeeD -
  EW647_RS13785 (EW647_14305) tsuA 2632080..2633138 (-) 1059 WP_000492332.1 thiosulfate utilization transporter TsuA/YeeE -
  EW647_RS13790 (EW647_14310) plaP 2633317..2634675 (-) 1359 WP_000019197.1 putrescine/proton symporter PlaP -
  EW647_RS13795 (EW647_14315) yoeI 2634665..2634727 (-) 63 WP_010723108.1 membrane protein YoeI -
  EW647_RS13800 (EW647_14320) yeeY 2634942..2635871 (-) 930 WP_000803351.1 LysR substrate-binding domain-containing protein -
  EW647_RS13805 (EW647_14325) yeeZ 2635917..2636741 (-) 825 WP_000754755.1 SDR family oxidoreductase -

Sequence

Protein


Download         Length: 475 a.a.        Molecular weight: 54498.80 Da        Isoelectric Point: 5.3498

>NTDB_id=304659 EW647_RS13775 WP_000980539.1 2630369..2631796(+) (sbcB) [Escherichia coli O145 strain RM12522-C8]
MMNDGKQQSTFLFHDYETFGTHPALDRPAQFAAIRTDDEFNVIGEPEVFYCKPADDYLPQPGAVLITGITPQEARAKGEN
EAAFAARIHSLFTVPKTCILGYNNVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDG
LPSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLMALIDVPQMKPLVHVSGMFGAWR
GNTSWVAPLAWHPENRNAVIMVDLAGDISPLLELDSDTLRERLYTAKADLGDNAAVPVKLVHINKCPVLAQANTLRPEDA
DRLGINRQHCLDNLKILRENPQVREKVVAIFVEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLPALDITFVD
KRIEKLLFNYRARNFPGTLDYAEQQRWLEHRRQVFTPEFLQGYAEEIQMLAQQYADDKEKVALLKALWQYAEEIV

Nucleotide


Download         Length: 1428 bp        

>NTDB_id=304659 EW647_RS13775 WP_000980539.1 2630369..2631796(+) (sbcB) [Escherichia coli O145 strain RM12522-C8]
ATGATGAATGACGGTAAGCAACAATCTACCTTTTTGTTTCACGATTACGAAACCTTTGGCACGCACCCCGCGTTAGATCG
CCCTGCACAGTTCGCAGCCATTCGCACCGATGACGAATTCAATGTCATCGGCGAACCCGAAGTCTTTTACTGCAAGCCCG
CGGATGACTATTTACCCCAGCCTGGAGCAGTATTAATTACCGGTATTACCCCGCAGGAGGCACGAGCGAAAGGAGAAAAC
GAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTACCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTT
CGACGACGAAGTCACACGCAACGTTTTTTATCGTAATTTCTACGATCCTTACGCCTGGAGCTGGCAGCATGATAACTCGC
GCTGGGATTTACTGGATGTTATGCGTGCCTGTTATGCCCTGCGCCCGGAAGGAATAAATTGGCCTGAAAATGATGACGGT
CTACCGAGCTTTCGCCTTGAGCATTTAACCAAAGCGAATGGTATTGAACATAGCAACGCCCACGATGCGATGGCTGATGT
GTACGCCACTATTGCGATGGCAAAACTGGTAAAAACGCGTCAGCCACGCCTGTTTGATTATCTCTTTACCCATCGTAATA
AACACAAACTAATGGCGTTGATTGATGTTCCGCAGATGAAACCCCTGGTTCACGTTTCCGGAATGTTTGGGGCATGGCGC
GGCAATACAAGCTGGGTGGCACCGCTGGCGTGGCATCCTGAAAATCGCAATGCCGTAATTATGGTGGATTTGGCAGGAGA
CATTTCGCCATTACTGGAGCTGGATAGCGACACATTGCGCGAGCGTTTATATACCGCAAAAGCCGATCTTGGCGATAACG
CCGCCGTTCCGGTTAAGCTGGTGCATATCAATAAATGTCCGGTACTGGCCCAGGCGAATACGCTACGCCCGGAAGATGCC
GACCGACTGGGAATTAATCGTCAGCATTGCCTCGATAACCTAAAAATTCTGCGTGAAAATCCGCAAGTGCGTGAAAAAGT
GGTAGCGATATTCGTGGAAGCCGAACCGTTTACACCTTCAGATAATGTGGATGCACAGCTTTATAACGGCTTTTTCAGTG
ACGCCGATCGTGCAGCAATGAAAATTGTGTTGGAAACCGAGCCGCGTAATTTACCGGCACTGGATATCACTTTTGTTGAT
AAACGAATTGAAAAGCTGTTGTTCAATTATCGGGCGCGCAACTTCCCGGGGACGCTGGATTATGCCGAGCAGCAACGCTG
GCTGGAGCACCGCCGCCAGGTCTTCACGCCAGAGTTTTTACAAGGCTATGCAGAAGAGATTCAGATGCTGGCGCAGCAGT
ATGCCGACGATAAAGAAAAAGTGGCGCTGTTAAAAGCGCTTTGGCAGTACGCGGAAGAGATTGTCTAA

Domains

Predicted by InterProScan.

Exonuclease (RNase_T)

(13-188)

Exonuclease C-terminal (ExoI_SH3)

(212-472)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  sbcB Vibrio cholerae O1 biovar El Tor strain E7946

58.723

98.947

0.581