Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF/pilF2   Type   Machinery gene
Locus tag   DV390_RS07225 Genome accession   NZ_CP031242
Coordinates   1436767..1437306 (-) Length   179 a.a.
NCBI ID   WP_042594885.1    Uniprot ID   -
Organism   Haemophilus influenzae strain M21460     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1431767..1442306
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DV390_RS07195 fdx 1432170..1432511 (+) 342 WP_005656373.1 ISC system 2Fe-2S type ferredoxin -
  DV390_RS07200 iscX 1432511..1432705 (+) 195 WP_005656371.1 Fe-S cluster assembly protein IscX -
  DV390_RS07205 - 1432756..1433370 (-) 615 WP_005654851.1 YfgM family protein -
  DV390_RS07210 hisS 1433388..1434659 (-) 1272 WP_042594883.1 histidine--tRNA ligase -
  DV390_RS07215 ispG 1434669..1435775 (-) 1107 WP_005649145.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  DV390_RS07220 - 1435784..1436695 (-) 912 WP_005669105.1 RodZ domain-containing protein -
  DV390_RS07225 pilF/pilF2 1436767..1437306 (-) 540 WP_042594885.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  DV390_RS07230 - 1437407..1438558 (-) 1152 WP_042594886.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  DV390_RS07235 - 1438805..1439683 (+) 879 WP_042594888.1 D-alanyl-D-alanine carboxypeptidase family protein -
  DV390_RS07240 - 1440048..1440929 (+) 882 WP_042594891.1 metal ABC transporter substrate-binding protein -
  DV390_RS07245 - 1440929..1441849 (+) 921 WP_005654835.1 ATP-binding cassette domain-containing protein -

Sequence

Protein


Download         Length: 179 a.a.        Molecular weight: 20557.14 Da        Isoelectric Point: 8.2626

>NTDB_id=304543 DV390_RS07225 WP_042594885.1 1436767..1437306(-) (pilF/pilF2) [Haemophilus influenzae strain M21460]
MKTISKQLSAVIFPFIFSACVSQSASSLNHQAAAKARVELALSYLQQNNPQLAKINLDKALQHDKNYYLVHSALAHYYQQ
QGEIENAHREYEIAVNLNHKQGDVHNNFGTFLCSQKKFEQAQQQFELALNSPNYYHQADTFENIVLCAYSSQKIDIYQQT
LEKLRQIDGKRAEKFNSLK

Nucleotide


Download         Length: 540 bp        

>NTDB_id=304543 DV390_RS07225 WP_042594885.1 1436767..1437306(-) (pilF/pilF2) [Haemophilus influenzae strain M21460]
ATGAAAACAATAAGTAAACAACTAAGTGCGGTTATTTTCCCTTTTATTTTTTCTGCTTGCGTTTCACAATCTGCATCTAG
CTTAAATCATCAAGCTGCCGCTAAAGCGCGTGTGGAACTCGCTTTGAGCTATCTTCAGCAAAATAATCCTCAACTGGCTA
AAATCAATTTAGACAAAGCACTTCAACACGATAAAAATTACTATCTCGTGCATTCAGCACTTGCACATTATTATCAACAA
CAAGGGGAAATAGAGAATGCACATCGTGAATATGAAATAGCCGTAAACCTTAATCATAAACAAGGCGATGTGCATAATAA
TTTTGGTACGTTTCTATGTAGTCAAAAGAAATTTGAACAAGCCCAGCAACAATTTGAATTAGCACTTAATTCGCCGAATT
ATTATCATCAAGCAGATACATTTGAAAATATCGTGCTTTGTGCTTATTCCTCGCAAAAAATAGATATTTATCAGCAAACT
TTAGAAAAATTACGTCAAATCGATGGAAAACGTGCGGAAAAATTCAATAGTCTAAAATAA

Domains


No domain identified.



Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF/pilF2 Haemophilus influenzae Rd KW20

97.207

100

0.972
  pilF Glaesserella parasuis strain SC1401

45.251

100

0.453

Multiple sequence alignment