Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   EWS90_RS26955 Genome accession   NZ_CP035739
Coordinates   5582568..5584061 (-) Length   497 a.a.
NCBI ID   WP_023128435.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain 1334/14     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5577568..5589061
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EWS90_RS26920 (EWS90_26920) - 5578419..5578868 (+) 450 WP_003094369.1 hypothetical protein -
  EWS90_RS26925 (EWS90_26925) - 5578881..5579492 (+) 612 WP_003098862.1 superoxide dismutase -
  EWS90_RS26930 (EWS90_26930) - 5579511..5580443 (+) 933 WP_003120918.1 ZIP family metal transporter -
  EWS90_RS26935 (EWS90_26935) - 5580540..5580812 (-) 273 WP_003094362.1 HPr family phosphocarrier protein -
  EWS90_RS26940 (EWS90_26940) rapZ 5580842..5581702 (-) 861 WP_003094361.1 RNase adapter RapZ -
  EWS90_RS26945 (EWS90_26945) ptsN 5581704..5582168 (-) 465 WP_003098853.1 PTS IIA-like nitrogen regulatory protein PtsN -
  EWS90_RS26950 (EWS90_26950) hpf 5582182..5582490 (-) 309 WP_003094359.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  EWS90_RS26955 (EWS90_26955) rpoN/rpoN1 5582568..5584061 (-) 1494 WP_023128435.1 RNA polymerase factor sigma-54 Machinery gene
  EWS90_RS26960 (EWS90_26960) lptB 5584287..5585012 (-) 726 WP_003094355.1 LPS export ABC transporter ATP-binding protein -
  EWS90_RS26965 (EWS90_26965) lptA 5585012..5585539 (-) 528 WP_003120916.1 lipopolysaccharide transport periplasmic protein LptA -
  EWS90_RS26970 (EWS90_26970) lptC 5585526..5586098 (-) 573 WP_034040549.1 LPS export ABC transporter periplasmic protein LptC -
  EWS90_RS26975 (EWS90_26975) - 5586107..5586646 (-) 540 WP_003098849.1 KdsC family phosphatase -
  EWS90_RS26980 (EWS90_26980) kdsD 5586646..5587626 (-) 981 WP_003094347.1 arabinose-5-phosphate isomerase KdsD -
  EWS90_RS26985 (EWS90_26985) - 5588040..5588849 (+) 810 WP_003094343.1 ABC transporter ATP-binding protein -

Sequence

Protein


Download         Length: 497 a.a.        Molecular weight: 56068.24 Da        Isoelectric Point: 4.4327

>NTDB_id=304541 EWS90_RS26955 WP_023128435.1 5582568..5584061(-) (rpoN/rpoN1) [Pseudomonas aeruginosa strain 1334/14]
MKPSLVLKMGQQLTMTPQLQQAIRLLQLSTLDLQQEIQEALESNPMLERQEDGDDFDNSDPLADGAEQAATAPQESPLQE
SATPSVESLDDDQWSERIPSELPVDTAWEDIYQTSASSLPSNDDDEWDFTARTSSGESLHSHLLWQVNLAPMSDTDRMIA
VTIIDSINNDGYLEESLEEILAAIDPELDVELDEVEVVLRRIQQLEPAGIGARNLRECLLLQLRQLPSTTPWLNEALRLV
SDYLDLLGGRDYSQLMRRMKLKEDELRQVIELIQCLHPRPGSQIESSEAEYIVPDVIVRKDNERWLVELNQEAMPRLRVN
ATYAGMVRRADSSADNTFMRNQLQEARWFIKSLQSRNETLMKVATQIVEHQRGFLDYGEEAMKPLVLHDIAEAVGMHEST
ISRVTTQKYMHTPRGIFELKYFFSSHVSTAEGGECSSTAIRAIIKKLVAAENAKKPLSDSKIAGLLEAQGIQVARRTVAK
YRESLGIAPSSERKRLV

Nucleotide


Download         Length: 1494 bp        

>NTDB_id=304541 EWS90_RS26955 WP_023128435.1 5582568..5584061(-) (rpoN/rpoN1) [Pseudomonas aeruginosa strain 1334/14]
ATGAAACCATCGCTAGTCCTCAAGATGGGCCAGCAGCTGACGATGACGCCGCAGCTGCAACAGGCCATACGCCTCCTCCA
GCTATCCACCCTGGACCTCCAGCAGGAAATCCAGGAAGCGCTGGAATCCAATCCCATGCTCGAACGCCAGGAAGACGGCG
ACGATTTCGACAACAGCGACCCTCTCGCCGATGGCGCCGAACAGGCGGCCACCGCGCCCCAGGAGAGCCCCCTCCAGGAA
AGCGCCACGCCCAGCGTCGAAAGCCTCGACGACGACCAGTGGAGCGAACGCATACCCAGCGAGTTGCCGGTGGATACCGC
CTGGGAAGACATCTACCAGACCAGCGCCAGCAGCCTGCCCAGCAACGACGATGACGAGTGGGACTTCACCGCGCGCACCA
GCAGCGGCGAGAGCCTGCACAGCCACCTGCTCTGGCAGGTCAACCTGGCACCGATGTCGGACACCGATCGGATGATCGCG
GTGACCATCATCGATAGCATCAACAACGATGGCTACCTCGAAGAGTCCCTCGAAGAAATCCTCGCCGCCATCGATCCGGA
ACTGGACGTCGAGCTCGACGAAGTGGAAGTCGTGCTGCGCCGCATCCAGCAACTGGAACCCGCCGGCATCGGCGCCCGCA
ACCTGCGCGAGTGCCTGCTGCTGCAACTGCGCCAGTTGCCGTCCACCACGCCCTGGCTGAACGAAGCGCTGCGCCTGGTA
TCCGACTACCTCGACCTGCTCGGCGGCCGCGACTACAGCCAATTGATGCGGCGCATGAAGCTCAAGGAAGACGAACTGCG
CCAGGTCATCGAGCTGATCCAGTGCCTGCATCCCCGGCCCGGTTCGCAGATCGAATCGAGCGAAGCCGAATACATCGTCC
CCGACGTCATCGTGCGCAAGGACAACGAGCGCTGGCTGGTCGAGCTGAACCAGGAGGCGATGCCACGCCTGCGCGTCAAC
GCCACCTACGCCGGCATGGTCCGCCGCGCCGACAGCAGCGCCGACAATACCTTCATGCGCAACCAGTTGCAGGAAGCCCG
CTGGTTCATCAAGAGCCTGCAGAGCCGCAACGAAACGCTGATGAAGGTCGCCACCCAGATCGTCGAGCACCAGCGCGGTT
TCCTCGACTACGGCGAAGAAGCCATGAAGCCGCTGGTCCTGCACGACATCGCCGAAGCGGTGGGCATGCACGAATCGACC
ATTTCGCGGGTCACCACGCAGAAATACATGCATACGCCGCGCGGCATTTTCGAGCTGAAATATTTCTTTTCAAGTCACGT
CAGTACCGCCGAAGGCGGGGAATGCTCGTCCACCGCGATCCGCGCGATCATCAAGAAACTGGTGGCCGCGGAAAATGCGA
AAAAGCCATTGAGCGACAGCAAGATCGCTGGTTTACTGGAGGCACAGGGCATTCAAGTGGCTCGCCGCACCGTCGCCAAA
TACCGCGAATCCCTGGGCATAGCCCCTTCGAGCGAGCGCAAGCGACTGGTGTGA

Domains

Predicted by InterProScan.

Sigma-54, DNA binding domain (Sigma54_DBD)

(337-495)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(129-323)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

46.654

100

0.477

Multiple sequence alignment