Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   EWS90_RS17930 Genome accession   NZ_CP035739
Coordinates   3665871..3667232 (-) Length   453 a.a.
NCBI ID   WP_009877389.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain 1334/14     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3660871..3672232
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EWS90_RS17895 (EWS90_17895) thiS 3661105..3661305 (-) 201 WP_003084514.1 sulfur carrier protein ThiS -
  EWS90_RS17900 (EWS90_17900) - 3661408..3661785 (-) 378 WP_003084513.1 DUF423 domain-containing protein -
  EWS90_RS17905 (EWS90_17905) mtgA 3661822..3662553 (+) 732 WP_003100972.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  EWS90_RS17910 (EWS90_17910) - 3662558..3663163 (-) 606 WP_003084511.1 hypothetical protein -
  EWS90_RS17915 (EWS90_17915) rpoH 3663228..3664082 (-) 855 WP_003084510.1 RNA polymerase sigma factor RpoH -
  EWS90_RS17920 (EWS90_17920) ftsX 3664196..3665203 (-) 1008 WP_003084508.1 permease-like cell division protein FtsX -
  EWS90_RS17925 (EWS90_17925) ftsE 3665203..3665874 (-) 672 WP_003084505.1 cell division ATP-binding protein FtsE -
  EWS90_RS17930 (EWS90_17930) pilA 3665871..3667232 (-) 1362 WP_009877389.1 signal recognition particle-docking protein FtsY Machinery gene
  EWS90_RS17935 (EWS90_17935) - 3667353..3668750 (+) 1398 WP_003084498.1 M16 family metallopeptidase -
  EWS90_RS17940 (EWS90_17940) - 3668743..3670230 (+) 1488 WP_003102862.1 M16 family metallopeptidase -
  EWS90_RS17945 (EWS90_17945) rsmD 3670230..3670826 (+) 597 WP_003102864.1 16S rRNA (guanine(966)-N(2))-methyltransferase RsmD -
  EWS90_RS17950 (EWS90_17950) - 3670829..3671395 (+) 567 WP_113887022.1 hypothetical protein -

Sequence

Protein


Download         Length: 453 a.a.        Molecular weight: 48165.59 Da        Isoelectric Point: 4.5525

>NTDB_id=304499 EWS90_RS17930 WP_009877389.1 3665871..3667232(-) (pilA) [Pseudomonas aeruginosa strain 1334/14]
MFGSNDDKKAPQGGEKKGLFGWWRKKPQAGEQPADQPVEPVSETAAAEQRAPADDVAQSLTEQPGRQQPSAAEPAEPPAP
VAEAPLAGDEPASAEEHSPRPEAPVAQPEPILAAEPEPEPEPEPEPEPVAPLAAAPAVSEPATRPGFFARLRQGLSKTSA
SIGEGMASLFLGRKEIDDDLLDDIETRLLTADVGVEATTLIVQNLTKRVARKELADSGALYKALQEELASLLRPVEQPLQ
VDVAREPYVILVVGVNGVGKTTTIGKLAKKLQLEGKKVMLAAGDTFRAAAVEQLQVWGERNRIPVIAQHTGADSASVIFD
AVQAAKARGIDVLIADTAGRLHTKDNLMEELKKVRRVIGKLDETAPHEVLLVLDAGTGQNAINQAKQFNLAVELTGLALT
KLDGTAKGGVIFALAKQFGLPIRYIGVGEGIDDLRTFEADAFVQALFAERENA

Nucleotide


Download         Length: 1362 bp        

>NTDB_id=304499 EWS90_RS17930 WP_009877389.1 3665871..3667232(-) (pilA) [Pseudomonas aeruginosa strain 1334/14]
ATGTTTGGTTCCAACGACGACAAGAAGGCCCCGCAGGGCGGGGAAAAGAAAGGTCTGTTCGGCTGGTGGCGGAAAAAGCC
GCAAGCCGGCGAGCAGCCTGCCGATCAGCCTGTCGAGCCGGTCTCCGAGACAGCGGCCGCCGAGCAGCGCGCGCCTGCCG
ATGACGTGGCGCAATCCCTGACAGAACAGCCAGGTCGGCAGCAGCCCTCCGCTGCCGAGCCGGCCGAGCCGCCGGCGCCG
GTTGCCGAAGCGCCGCTCGCCGGTGACGAGCCAGCGTCGGCCGAGGAGCATTCGCCCCGGCCCGAGGCGCCCGTCGCGCA
ACCCGAGCCTATCCTGGCTGCCGAGCCCGAGCCCGAGCCCGAGCCCGAGCCCGAGCCCGAGCCCGTTGCGCCGCTGGCCG
CCGCTCCGGCAGTCAGCGAGCCGGCGACCAGGCCGGGCTTCTTCGCCCGCCTCCGGCAGGGGCTGTCCAAGACCAGCGCC
AGCATTGGCGAAGGCATGGCCAGCCTGTTCCTCGGTCGCAAGGAAATCGACGACGACCTGCTCGACGACATCGAGACGCG
CCTGCTGACCGCCGACGTCGGCGTCGAGGCCACCACCCTGATCGTGCAGAACCTGACCAAGCGCGTGGCGCGCAAAGAGC
TGGCCGACAGCGGCGCGCTGTACAAGGCGCTGCAGGAAGAGCTGGCCAGCCTGCTGCGCCCGGTGGAGCAGCCGTTGCAG
GTCGACGTCGCGCGCGAGCCCTATGTGATCCTGGTGGTCGGGGTGAACGGCGTGGGCAAGACCACCACCATCGGCAAGCT
GGCGAAGAAGCTGCAACTCGAGGGCAAGAAGGTCATGCTGGCCGCCGGCGACACCTTCCGCGCCGCCGCCGTGGAGCAGT
TGCAGGTCTGGGGCGAGCGCAACCGCATCCCGGTGATCGCCCAGCACACCGGCGCCGACTCCGCCTCGGTGATCTTCGAC
GCGGTGCAGGCCGCCAAGGCCCGCGGCATCGACGTGCTGATCGCCGATACCGCCGGACGCCTGCATACCAAGGACAACCT
GATGGAGGAGCTGAAGAAGGTGCGCCGGGTGATCGGCAAGCTGGACGAGACGGCGCCCCATGAGGTCCTGCTGGTGCTCG
ACGCCGGCACCGGGCAGAACGCCATCAACCAGGCCAAGCAGTTCAACCTCGCGGTGGAGCTGACCGGCCTTGCCCTGACC
AAACTGGACGGCACCGCCAAGGGCGGGGTGATCTTCGCCCTGGCCAAGCAGTTCGGCCTGCCGATCCGCTATATCGGTGT
CGGCGAGGGGATCGACGATCTCCGTACCTTCGAGGCCGACGCCTTCGTCCAGGCACTCTTCGCAGAGCGGGAGAACGCAT
GA

Domains

Predicted by InterProScan.

SRP54-type protein, GTPase domain (SRP54)

(248-447)

SRP54-type protein, helical bundle domain (SRP54_N)

(163-228)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Neisseria gonorrhoeae MS11

47.126

96.026

0.453