Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF/pilF2   Type   Machinery gene
Locus tag   DV427_RS05880 Genome accession   NZ_CP031240
Coordinates   1164845..1165387 (-) Length   180 a.a.
NCBI ID   WP_114891630.1    Uniprot ID   A0A502JS60
Organism   Haemophilus haemolyticus strain M19345     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1159845..1170387
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DV427_RS05850 fdx 1160250..1160591 (+) 342 WP_005629440.1 ISC system 2Fe-2S type ferredoxin -
  DV427_RS05855 iscX 1160591..1160785 (+) 195 WP_005630102.1 Fe-S cluster assembly protein IscX -
  DV427_RS05860 - 1160836..1161450 (-) 615 WP_114891627.1 YfgM family protein -
  DV427_RS05865 hisS 1161468..1162739 (-) 1272 WP_114891628.1 histidine--tRNA ligase -
  DV427_RS05870 ispG 1162749..1163855 (-) 1107 WP_005630108.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  DV427_RS05875 - 1163867..1164775 (-) 909 WP_114891629.1 RodZ domain-containing protein -
  DV427_RS05880 pilF/pilF2 1164845..1165387 (-) 543 WP_114891630.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  DV427_RS05885 - 1165485..1166636 (-) 1152 WP_005630119.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  DV427_RS05890 - 1166883..1167761 (+) 879 WP_114891632.1 D-alanyl-D-alanine carboxypeptidase family protein -
  DV427_RS05895 - 1167841..1168308 (-) 468 WP_114891633.1 hypothetical protein -
  DV427_RS05900 - 1168666..1169547 (+) 882 WP_005637458.1 metal ABC transporter substrate-binding protein -

Sequence

Protein


Download         Length: 180 a.a.        Molecular weight: 20825.35 Da        Isoelectric Point: 7.8274

>NTDB_id=304480 DV427_RS05880 WP_114891630.1 1164845..1165387(-) (pilF/pilF2) [Haemophilus haemolyticus strain M19345]
MQTISKQLSAVIFPFIFSACVSQSTIQDFNQQSAAKARVELALGYLQQNNPQLAKINLDKALQHDKNYYLVHSALAHYYQ
QQGEIDNAHREYETALKLNNQQGDLHNNFGTFLCRQKQFEQAQQQFQLALNSPNYYHQADTFENMALCAYSAQKMDIYQQ
ALQKLLQTDSKRAEKFNTFK

Nucleotide


Download         Length: 543 bp        

>NTDB_id=304480 DV427_RS05880 WP_114891630.1 1164845..1165387(-) (pilF/pilF2) [Haemophilus haemolyticus strain M19345]
ATGCAAACAATAAGTAAACAACTAAGTGCGGTTATTTTCCCTTTCATTTTTTCTGCTTGCGTTTCCCAATCAACCATTCA
AGATTTCAATCAACAATCAGCAGCTAAAGCTCGCGTAGAACTTGCACTAGGCTACCTTCAACAAAATAATCCTCAACTCG
CTAAAATTAACTTAGATAAAGCACTTCAACACGATAAAAATTACTATCTCGTGCATTCAGCGCTTGCGCATTATTATCAA
CAACAAGGGGAAATAGATAATGCACATCGTGAATATGAAACAGCGTTAAAACTCAATAATCAACAGGGAGACTTACATAA
TAACTTTGGTACATTTTTATGTCGCCAAAAGCAATTTGAACAAGCTCAGCAGCAATTTCAACTCGCACTGAATTCACCTA
ATTATTATCATCAAGCAGATACATTTGAAAATATGGCTCTTTGTGCTTATTCCGCTCAAAAAATGGATATTTACCAACAA
GCATTACAAAAATTGCTACAAACTGATAGTAAACGAGCGGAAAAATTTAATACCTTCAAATAA

Domains


No domain identified.



Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A502JS60

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF/pilF2 Haemophilus influenzae Rd KW20

83.333

100

0.833
  pilF Glaesserella parasuis strain SC1401

47.253

100

0.478

Multiple sequence alignment