Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   EPB55_RS07630 Genome accession   NZ_CP035731
Coordinates   1498589..1500313 (-) Length   574 a.a.
NCBI ID   WP_011079397.1    Uniprot ID   A0A3Q0L3L1
Organism   Vibrio vulnificus strain 2142-77     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1493589..1505313
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EPB55_RS07595 - 1493957..1494646 (-) 690 WP_011079404.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  EPB55_RS07600 - 1494646..1495212 (-) 567 WP_011079403.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  EPB55_RS07605 - 1495212..1495697 (-) 486 WP_072612420.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  EPB55_RS23990 - 1495757..1496203 (-) 447 WP_108653853.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  EPB55_RS07615 - 1496237..1496815 (-) 579 WP_011079400.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  EPB55_RS07620 - 1496894..1497355 (-) 462 WP_039554781.1 hypothetical protein -
  EPB55_RS07625 - 1497359..1498579 (-) 1221 WP_011079398.1 type II secretion system F family protein -
  EPB55_RS07630 pilF 1498589..1500313 (-) 1725 WP_011079397.1 GspE/PulE family protein Machinery gene
  EPB55_RS07635 - 1500310..1501431 (-) 1122 WP_103277913.1 tetratricopeptide repeat protein -
  EPB55_RS07640 - 1501428..1502276 (-) 849 WP_103277912.1 AAA family ATPase -
  EPB55_RS07645 mshL 1502284..1503930 (-) 1647 WP_072605704.1 pilus (MSHA type) biogenesis protein MshL -
  EPB55_RS07650 - 1503946..1504266 (-) 321 WP_011079393.1 MSHA biogenesis protein MshK -
  EPB55_RS07655 gspM 1504259..1504909 (-) 651 WP_045596391.1 type II secretion system protein GspM -

Sequence

Protein


Download         Length: 574 a.a.        Molecular weight: 63672.03 Da        Isoelectric Point: 5.3226

>NTDB_id=304396 EPB55_RS07630 WP_011079397.1 1498589..1500313(-) (pilF) [Vibrio vulnificus strain 2142-77]
MKVRLRKRLGDLLVEEGIINEAQLDQALGAQRSGGRKLGDTLIDLGFLSEQQMLAFLSQQLDLPLIDLNRATVDIDAVQL
LPEVHARRLRALVIGRNGDTLRIAMSDPADLFAQEALIGQLGQYGIEFVIAPERQLVEGFDRYYRRTKEIANFAEQLQAE
HQVNDAFDFNIEDDDSDEVTVVKLINSMFEDAIQVGASDIHIEPDANVLRLRQRIDGVLHETLLNEVNIAPALVLRLKLM
ANLDISEKRLPQDGRFNIRSKGQSVDIRMSTMPVQYGESVVMRLLNQSSGVRKLEDSGLPDDLLVRLRRQLKRPHGMILV
TGPTGSGKTTTLYGALTELNQPGKKIITAEDPVEYRLSRVNQVQINPKINLDFSTVLRTFLRQDPDIILIGEMRDHETVE
IGLRAALTGHLVLSTLHTNDAVDSALRMIDMGAPGYLVASAVRAVVAQRLVRRVCPDCKTEDHVGESRKAWLAQRFPNQV
EAGFVKGRGCQNCNLTGYRGRIGVFEMLELEHGMMDALRANDAVEFAHAARRSPGYKPLLASAMELALQGVVSLDEVMNL
GEGDSSGVAEAIIM

Nucleotide


Download         Length: 1725 bp        

>NTDB_id=304396 EPB55_RS07630 WP_011079397.1 1498589..1500313(-) (pilF) [Vibrio vulnificus strain 2142-77]
ATGAAAGTAAGATTAAGAAAGCGGCTTGGTGACCTTCTTGTTGAGGAAGGCATTATCAATGAAGCGCAACTTGATCAAGC
ACTCGGCGCGCAAAGAAGTGGTGGACGCAAGCTTGGCGATACGCTGATTGATTTGGGTTTTTTGTCTGAACAACAGATGT
TGGCGTTTTTATCGCAGCAACTTGATCTGCCTTTGATTGACTTAAATCGAGCCACGGTCGATATCGATGCAGTGCAGTTG
CTACCAGAAGTGCATGCTCGTCGTTTACGCGCATTGGTGATTGGCCGTAATGGCGACACGTTGCGCATTGCGATGAGTGA
TCCAGCGGACCTTTTTGCGCAAGAAGCGTTGATCGGTCAGCTAGGACAATATGGCATTGAGTTTGTTATCGCGCCAGAAA
GACAATTGGTGGAAGGCTTTGATCGCTATTATCGCCGTACCAAAGAGATCGCCAACTTTGCCGAGCAGTTACAAGCGGAG
CACCAAGTCAACGATGCATTCGATTTCAATATCGAAGATGACGACAGTGATGAGGTGACCGTCGTTAAGCTCATCAACTC
GATGTTTGAAGACGCCATCCAGGTGGGCGCATCGGATATCCATATCGAACCGGATGCCAATGTGTTGCGCTTGCGTCAAC
GTATTGATGGCGTGTTGCACGAAACCTTGCTGAATGAAGTCAACATCGCGCCAGCATTGGTACTGAGATTGAAACTCATG
GCCAACTTGGATATTTCAGAAAAGCGTCTGCCGCAAGATGGCCGTTTTAACATCCGATCCAAAGGTCAGTCGGTGGATAT
CCGTATGTCGACCATGCCAGTTCAGTATGGTGAGTCGGTGGTAATGCGTTTGCTCAATCAATCTTCCGGAGTCAGAAAAT
TAGAAGACTCCGGTTTGCCGGACGATCTTTTAGTGCGCCTACGCCGACAGTTGAAACGTCCGCACGGCATGATCCTTGTG
ACTGGCCCTACGGGCTCCGGTAAAACCACCACGCTGTATGGTGCTCTAACAGAATTAAACCAACCGGGGAAAAAGATCAT
CACAGCGGAAGATCCGGTTGAATACCGACTCTCGCGTGTCAATCAAGTGCAGATCAATCCGAAGATCAACCTTGATTTTT
CCACGGTGTTAAGAACATTCCTCCGTCAGGATCCCGATATCATTTTGATTGGTGAGATGCGTGACCATGAAACGGTTGAA
ATTGGCCTGCGAGCGGCGCTGACCGGTCACTTAGTATTGAGTACCTTACACACCAATGACGCCGTAGACAGTGCTCTTCG
TATGATTGATATGGGTGCTCCGGGTTATCTGGTCGCGAGTGCCGTACGTGCTGTGGTCGCGCAGCGATTGGTGCGCCGTG
TCTGTCCGGATTGCAAAACGGAAGATCATGTTGGAGAGTCACGCAAAGCGTGGTTGGCACAACGCTTCCCGAATCAAGTG
GAAGCAGGCTTTGTGAAAGGGCGCGGTTGTCAAAACTGTAACTTAACAGGTTATCGCGGCCGTATTGGTGTGTTTGAAAT
GCTGGAACTTGAACATGGAATGATGGACGCGTTGCGCGCCAATGATGCCGTTGAGTTTGCACATGCTGCGCGTCGTTCGC
CAGGCTATAAACCTCTGTTAGCGTCGGCAATGGAGTTGGCGCTACAAGGCGTAGTGAGCTTAGACGAAGTGATGAATTTA
GGCGAAGGTGACTCCTCTGGGGTTGCTGAAGCGATCATTATGTAA

Domains

Predicted by InterProScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-148)

Type II/IV secretion system protein (T2SSE)

(187-453)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A3Q0L3L1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Thermus thermophilus HB27

38.104

97.387

0.371
  pilB Vibrio cholerae strain A1552

37.699

98.432

0.371

Multiple sequence alignment