Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   EPB54_RS16825 Genome accession   NZ_CP035701
Coordinates   3342715..3343932 (-) Length   405 a.a.
NCBI ID   WP_005481951.1    Uniprot ID   Q87KM1
Organism   Vibrio parahaemolyticus strain 2012AW-0154     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3337715..3348932
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EPB54_RS16795 - 3337900..3338310 (+) 411 WP_015297469.1 flagellar basal body-associated protein FliL -
  EPB54_RS16800 glpG 3338370..3339212 (-) 843 WP_005496414.1 rhomboid family intramembrane serine protease GlpG -
  EPB54_RS16805 glpE 3339212..3339532 (-) 321 WP_005460380.1 thiosulfate sulfurtransferase GlpE -
  EPB54_RS16810 rpoH 3340024..3340884 (-) 861 WP_005490420.1 RNA polymerase sigma factor RpoH -
  EPB54_RS16815 ftsX 3341060..3342028 (-) 969 WP_005481956.1 permease-like cell division protein FtsX -
  EPB54_RS16820 ftsE 3342018..3342692 (-) 675 WP_005481953.1 cell division ATP-binding protein FtsE -
  EPB54_RS16825 pilA 3342715..3343932 (-) 1218 WP_005481951.1 signal recognition particle-docking protein FtsY Machinery gene
  EPB54_RS16830 rsmD 3344156..3344755 (+) 600 WP_005455148.1 16S rRNA (guanine(966)-N(2))-methyltransferase RsmD -
  EPB54_RS16835 - 3344781..3345047 (+) 267 WP_005496405.1 DUF1145 domain-containing protein -
  EPB54_RS16840 - 3345100..3345684 (-) 585 WP_005455144.1 YhgN family NAAT transporter -
  EPB54_RS16845 - 3345804..3346025 (-) 222 WP_015297472.1 YecH family metal-binding protein -
  EPB54_RS16850 - 3346095..3346715 (-) 621 WP_005481960.1 lysoplasmalogenase -
  EPB54_RS26650 - 3347449..3347628 (+) 180 WP_005458791.1 hypothetical protein -
  EPB54_RS16865 - 3347692..3348060 (-) 369 WP_005489749.1 hypothetical protein -
  EPB54_RS16870 - 3348302..3348631 (+) 330 WP_015297474.1 DUF2500 domain-containing protein -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 44154.75 Da        Isoelectric Point: 4.2881

>NTDB_id=304279 EPB54_RS16825 WP_005481951.1 3342715..3343932(-) (pilA) [Vibrio parahaemolyticus strain 2012AW-0154]
MTEKKKRGLLSWLGFGDEEQSPKPKTEETVTEEAVEAPSEEQQTEEVAEQAQQTQELEQEPEKAEAAPAEAEAEAEAEAE
AEEPQVPVAPRIQEQEKPTESFFARLKRSLSRTKANIGAGFFGLFSGKKIDDDLFEELEEQLLIADVGMDTTTKIINNLT
EKASRGDLKDGEALYGLLKEEMAEILSKVEQPLEIDSSKTPYVILMVGVNGVGKTTTIGKLAKQFQSQGKKVMLAAGDTF
RAAAVEQLQVWGERNNVPVIAQHTGADSASVIYDAIEAAKARGVDVVIADTAGRLQNKANLMEELRKIVRVMKKIDDSAP
HEIMLTLDAGTGQNAISQAKLFSDVAPLTGITLTKLDGTAKGGVIFAIADQFSIPIRYIGVGEGIEDLRPFETQEFIDAL
FSREE

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=304279 EPB54_RS16825 WP_005481951.1 3342715..3343932(-) (pilA) [Vibrio parahaemolyticus strain 2012AW-0154]
ATGACGGAAAAAAAGAAGCGCGGATTACTTTCGTGGCTAGGTTTTGGTGACGAAGAACAAAGCCCAAAACCAAAAACTGA
AGAAACAGTAACAGAAGAAGCAGTGGAAGCGCCTTCTGAAGAACAGCAAACCGAAGAGGTTGCTGAGCAAGCGCAACAAA
CTCAAGAGCTTGAACAAGAGCCAGAAAAAGCTGAGGCTGCCCCAGCAGAAGCAGAAGCAGAAGCAGAAGCAGAAGCAGAA
GCAGAAGAGCCACAAGTACCGGTTGCGCCTCGTATTCAAGAGCAAGAAAAGCCGACAGAAAGCTTCTTCGCTCGCCTTAA
GCGCAGCCTAAGCCGTACGAAAGCAAACATCGGTGCTGGTTTCTTTGGTCTGTTCAGCGGTAAAAAAATCGATGACGACC
TATTTGAAGAGCTAGAAGAGCAACTGCTCATTGCCGATGTGGGCATGGACACCACCACAAAAATCATCAACAACCTGACA
GAAAAAGCCTCTCGAGGTGATCTGAAAGATGGCGAAGCCCTTTATGGTCTGCTGAAAGAAGAAATGGCGGAGATTCTATC
TAAAGTAGAACAGCCTCTAGAGATCGACAGCAGCAAAACACCTTACGTCATCTTGATGGTTGGTGTGAATGGTGTGGGTA
AAACCACCACCATCGGTAAACTTGCGAAGCAGTTCCAAAGCCAAGGCAAGAAAGTGATGTTAGCGGCTGGCGATACTTTC
CGAGCGGCTGCAGTTGAGCAGCTGCAAGTGTGGGGCGAGCGTAACAATGTTCCTGTGATCGCGCAGCATACTGGCGCAGA
CAGCGCATCGGTTATCTACGATGCGATTGAAGCGGCAAAAGCGCGTGGCGTCGATGTGGTTATCGCCGATACGGCGGGTC
GTCTGCAAAACAAAGCCAACCTAATGGAAGAGCTACGCAAGATTGTACGTGTGATGAAGAAAATTGATGATTCTGCACCA
CATGAAATCATGCTGACGCTGGATGCGGGTACTGGCCAAAATGCGATTAGCCAAGCGAAACTTTTCAGTGATGTAGCTCC
TCTAACCGGGATTACATTGACTAAGCTGGATGGTACAGCGAAGGGCGGCGTTATTTTCGCTATCGCTGATCAGTTTAGTA
TTCCAATTCGCTACATTGGTGTAGGCGAAGGCATTGAAGACTTGCGTCCTTTTGAAACTCAAGAGTTTATCGACGCTTTG
TTTAGCCGTGAAGAGTAA

Domains

Predicted by InterProScan.

SRP54-type protein, GTPase domain (SRP54)

(202-401)

SRP54-type protein, helical bundle domain (SRP54_N)

(121-182)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q87KM1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Neisseria gonorrhoeae MS11

49.731

91.852

0.457