Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   PB01_RS19800 Genome accession   NZ_CP031223
Coordinates   4163390..4164760 (-) Length   456 a.a.
NCBI ID   WP_151701761.1    Uniprot ID   A0A5J6STV1
Organism   Psychrobacillus glaciei strain PB01     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 4158390..4169760
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PB01_RS19780 (PB01_19980) gltX 4159397..4160860 (-) 1464 WP_151701757.1 glutamate--tRNA ligase -
  PB01_RS19785 (PB01_19985) ispF 4160931..4161410 (-) 480 WP_151701758.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -
  PB01_RS19790 (PB01_19990) ispD 4161415..4162104 (-) 690 WP_151701759.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  PB01_RS19795 (PB01_19995) - 4162175..4163263 (-) 1089 WP_151701760.1 PIN/TRAM domain-containing protein -
  PB01_RS19800 (PB01_20000) radA 4163390..4164760 (-) 1371 WP_151701761.1 DNA repair protein RadA Machinery gene
  PB01_RS19805 (PB01_20005) - 4164819..4167266 (-) 2448 WP_151701762.1 ATP-dependent Clp protease ATP-binding subunit -
  PB01_RS19810 (PB01_20010) - 4167263..4168357 (-) 1095 WP_151701763.1 protein arginine kinase -
  PB01_RS19815 (PB01_20015) - 4168347..4168892 (-) 546 WP_151701764.1 UvrB/UvrC motif-containing protein -
  PB01_RS19820 (PB01_20020) - 4168914..4169381 (-) 468 WP_151701765.1 CtsR family transcriptional regulator -

Sequence

Protein


Download         Length: 456 a.a.        Molecular weight: 50095.79 Da        Isoelectric Point: 6.9000

>NTDB_id=304212 PB01_RS19800 WP_151701761.1 4163390..4164760(-) (radA) [Psychrobacillus glaciei strain PB01]
MAKRKTKFMCNSCGYESAKWMGRCPGCGEWNTMVEELEVVSKGPKRSFQHSTGVAQKATPIIAVETADEPRVETELMELN
RVLGGGIVPGSLILIGGDPGIGKSTLLLQVSALLANKGERVLYISGEESIRQTKLRAERLGVTSAELYIYSETNLELINE
TIDSVEPRFVIVDSIQTVHHPEVTSAPGSVSQVRECTAELMRIAKTKNIAIFLVGHVTKEGQIAGPRLLEHMVDTVLYFE
GERHHTYRILRSQKNRFGSTNEIAIFEMVQQGLKEVLNPSELFLQERSHGAAGSAIVASMEGTRPILVEIQALVTQSSFN
YPKRMATGIDQNRISLLMAVLEKRMGLLLQTQDAYIKVAGGVKLDEPAIDLAVLVSIVSSFRDTGVAPTDCFVGEVGLTG
EVRRVSRIEQRVQEATKLGFKRIVIPASNLGGWDYPKGIQVIGVETVNEVMKEAFR

Nucleotide


Download         Length: 1371 bp        

>NTDB_id=304212 PB01_RS19800 WP_151701761.1 4163390..4164760(-) (radA) [Psychrobacillus glaciei strain PB01]
ATGGCAAAACGAAAAACGAAGTTTATGTGTAATTCTTGCGGTTATGAGTCTGCCAAATGGATGGGAAGATGTCCAGGATG
CGGAGAATGGAATACGATGGTAGAAGAGTTGGAGGTTGTTTCAAAAGGACCAAAACGATCTTTTCAACATTCTACTGGAG
TAGCTCAGAAAGCCACACCAATTATTGCGGTGGAGACAGCGGATGAACCGAGAGTAGAAACGGAACTCATGGAGTTGAAT
CGAGTACTTGGCGGTGGAATTGTGCCTGGTTCCCTTATATTAATAGGTGGAGATCCGGGAATCGGAAAGTCGACTTTGCT
TTTACAAGTTTCTGCACTGCTCGCAAATAAGGGAGAGCGCGTGTTATATATTTCAGGTGAGGAATCGATTAGACAGACGA
AGCTTCGCGCCGAGCGTTTGGGTGTCACATCTGCTGAACTGTATATATATTCTGAAACAAATTTAGAATTAATCAATGAA
ACGATTGATAGTGTGGAGCCTAGATTTGTTATTGTCGACTCCATTCAAACTGTACATCATCCGGAAGTGACAAGTGCTCC
AGGAAGTGTGTCTCAAGTGCGGGAATGTACAGCTGAACTAATGCGCATTGCTAAAACAAAAAATATAGCAATTTTCTTAG
TAGGACATGTAACGAAAGAGGGACAAATTGCTGGACCACGTTTATTGGAACATATGGTAGATACGGTTTTATATTTTGAG
GGAGAACGACATCATACATACCGAATTTTACGGTCACAAAAAAATAGATTTGGTTCAACAAATGAAATTGCTATTTTTGA
GATGGTGCAGCAAGGTTTAAAAGAAGTGTTGAATCCATCTGAACTATTTTTGCAAGAGCGTTCACACGGAGCAGCTGGCT
CAGCCATTGTTGCTTCGATGGAAGGGACAAGACCCATCTTGGTTGAAATTCAAGCACTAGTTACGCAATCTAGTTTTAAT
TATCCAAAACGAATGGCAACAGGAATCGATCAAAATAGAATTTCTTTACTCATGGCAGTATTAGAAAAACGTATGGGATT
ACTTTTGCAAACACAAGATGCTTATATAAAAGTTGCGGGTGGAGTTAAATTAGACGAACCTGCGATTGATTTAGCCGTCC
TAGTAAGTATCGTATCCAGTTTCCGGGACACAGGAGTAGCACCTACCGATTGTTTTGTGGGGGAGGTAGGACTAACTGGC
GAAGTGAGACGAGTATCGAGAATTGAGCAAAGAGTGCAAGAGGCGACCAAATTAGGTTTTAAACGCATCGTGATTCCTGC
ATCCAATTTAGGTGGCTGGGATTATCCAAAAGGTATTCAAGTAATTGGGGTAGAAACGGTAAATGAAGTCATGAAAGAAG
CTTTTCGGTAA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(71-221)

Subunit ChlI of Mg-chelatase (ChlI)

(362-429)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5J6STV1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

71.179

100

0.715
  radA Streptococcus pneumoniae Rx1

63.077

99.781

0.629
  radA Streptococcus pneumoniae D39

63.077

99.781

0.629
  radA Streptococcus pneumoniae R6

63.077

99.781

0.629
  radA Streptococcus pneumoniae TIGR4

63.077

99.781

0.629
  radA Streptococcus mitis NCTC 12261

63.077

99.781

0.629
  radA Streptococcus mitis SK321

62.857

99.781

0.627

Multiple sequence alignment