Detailed information    

insolico Bioinformatically predicted

Overview

Name   recJ   Type   Machinery gene
Locus tag   EWG56_RS14145 Genome accession   NZ_CP035671
Coordinates   2739802..2742075 (+) Length   757 a.a.
NCBI ID   WP_000595016.1    Uniprot ID   -
Organism   Staphylococcus aureus strain VB31683     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2734802..2747075
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EWG56_RS14130 (EWG56_14130) tgt 2735630..2736769 (+) 1140 WP_001112045.1 tRNA guanosine(34) transglycosylase Tgt -
  EWG56_RS14135 (EWG56_14135) yajC 2736788..2737048 (+) 261 WP_001160682.1 preprotein translocase subunit YajC -
  EWG56_RS14140 (EWG56_14140) secDF 2737320..2739599 (+) 2280 WP_000749795.1 protein translocase subunit SecDF -
  EWG56_RS14145 (EWG56_14145) recJ 2739802..2742075 (+) 2274 WP_000595016.1 single-stranded-DNA-specific exonuclease RecJ Machinery gene
  EWG56_RS14150 (EWG56_14150) - 2742097..2742615 (+) 519 WP_000364542.1 adenine phosphoribosyltransferase -
  EWG56_RS14155 (EWG56_14155) - 2743043..2745232 (+) 2190 WP_001058583.1 bifunctional (p)ppGpp synthetase/guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase -
  EWG56_RS14160 (EWG56_14160) dtd 2745244..2745696 (+) 453 WP_000869983.1 D-aminoacyl-tRNA deacylase -
  EWG56_RS14165 (EWG56_14165) - 2745693..2746568 (+) 876 WP_000717800.1 N-acetylmuramoyl-L-alanine amidase -

Sequence

Protein


Download         Length: 757 a.a.        Molecular weight: 85622.87 Da        Isoelectric Point: 4.9088

>NTDB_id=304191 EWG56_RS14145 WP_000595016.1 2739802..2742075(+) (recJ) [Staphylococcus aureus strain VB31683]
MIKPKYKWKLTKPAEYISDELTSKLKLTPIVKKILESKSIIDEQTIEAIISDTDINHDALQLSDMTKTIERIKRAIANDE
KILVYGDYDADGVTSTTILVTTLQLLGAQVGWHIPNRFTEGYGPNELAFRNAHDEGITLIITVDNGIQGHNEIKMVQDLG
VDVIVTDHHEIGSTLPEAYAIVHPMHPSFNYPFQQLCGAGVAYKLAQALIENVPDYFKALVAIGTIADLVSLTDENRSLV
KQGLKVLNDQCPTSVKALLKEAGYNDNIDEETIGFIIGPRLNAVGRLDDASLACELLMTDDEEEAAFLAEQVEHFNRERK
DIVATITEEAMAMAETKVKKGDLFLLLAKENWHEGVLGIVASKIVETFALPTLILNIDREQNHAKGSARSIDQVSMFEIL
SAHQELIAKFGGHHMAAGMTMDIENIESLAEGLNKWMKELSKTTSLDPVKPVDVLLTENDITIKNIRDMNRLRPFGTDFS
RPIFEMDDLSVSSVKAIGQQKNHLKLTLGESNIAALFWQNGHLEPELQDEQPINILGSVQINEWNGNQSPQLIIQDIAMN
EQQILDYRSKRKSLPFTENDENIVVLIHPKSDKVNANEYYYGEEIKHQTDKVVLRDLPTSMEDLSNSLQQLQFSQLYIVL
QHNHSIYFDGIPNMDIFKKCYKALITKQETNIQKEGMLLCQHLSVKPDTLKFMLKVFLDLKFVTQEDGLIRINQQPDKRS
IDSSKVYQLRQQRMDVEKQLLYQDFSEIKNWIKSQLS

Nucleotide


Download         Length: 2274 bp        

>NTDB_id=304191 EWG56_RS14145 WP_000595016.1 2739802..2742075(+) (recJ) [Staphylococcus aureus strain VB31683]
ATGATTAAACCGAAGTATAAGTGGAAATTAACTAAACCAGCTGAATACATAAGTGATGAATTAACATCAAAATTAAAGTT
AACGCCAATCGTAAAAAAAATATTAGAAAGTAAATCGATTATTGATGAACAAACAATAGAAGCCATCATCTCAGATACAG
ATATCAATCATGATGCACTGCAATTGAGCGATATGACTAAGACTATTGAAAGAATTAAAAGAGCGATAGCGAATGATGAG
AAAATCCTAGTTTATGGTGATTACGATGCCGACGGTGTTACCTCAACTACAATTTTAGTGACAACATTACAACTTTTAGG
TGCACAAGTAGGATGGCATATTCCTAACCGCTTTACCGAAGGATATGGACCAAACGAACTAGCATTTAGAAATGCTCATG
ATGAAGGCATAACATTAATCATAACTGTTGATAATGGCATACAAGGTCATAATGAAATTAAAATGGTTCAAGATTTAGGA
GTTGATGTCATTGTAACTGATCATCATGAAATTGGTAGTACATTACCAGAAGCATATGCAATCGTACATCCAATGCATCC
ATCGTTTAATTATCCGTTCCAACAATTGTGTGGTGCAGGTGTTGCGTACAAATTAGCACAAGCATTGATTGAAAATGTAC
CTGATTATTTTAAAGCATTAGTTGCGATAGGTACCATTGCGGATTTAGTATCCTTAACTGATGAAAATCGATCATTAGTG
AAACAAGGATTAAAAGTCTTAAATGATCAATGTCCGACCTCTGTTAAAGCGTTGCTAAAAGAAGCGGGATACAATGACAA
TATTGATGAAGAAACAATTGGTTTTATTATTGGACCTAGACTTAATGCTGTAGGTCGTTTAGATGACGCATCATTAGCTT
GCGAATTGTTGATGACTGACGACGAAGAAGAAGCTGCATTTTTAGCAGAACAAGTTGAACATTTTAACCGTGAAAGAAAA
GATATTGTAGCAACTATCACAGAAGAAGCAATGGCTATGGCAGAAACGAAAGTTAAAAAGGGAGATTTATTTTTACTTTT
AGCAAAAGAAAATTGGCATGAAGGTGTCTTAGGTATTGTTGCTTCGAAAATTGTCGAAACATTTGCTTTGCCAACATTAA
TTTTAAATATTGATAGGGAGCAAAATCATGCAAAAGGTTCCGCCAGATCAATTGATCAAGTGTCGATGTTTGAAATACTA
TCAGCACATCAAGAGTTAATCGCTAAATTTGGCGGCCATCATATGGCTGCAGGTATGACGATGGATATTGAAAATATAGA
ATCGTTAGCTGAAGGTTTAAATAAATGGATGAAAGAATTATCTAAAACAACTTCGTTAGATCCCGTAAAGCCAGTAGACG
TATTATTAACAGAAAATGATATAACGATTAAAAATATTCGTGATATGAATCGTTTAAGACCCTTCGGAACAGATTTTTCT
AGACCTATTTTTGAAATGGATGATTTGTCAGTTTCTTCAGTTAAGGCGATCGGTCAACAAAAAAATCATCTAAAATTGAC
ACTTGGTGAAAGTAATATCGCCGCGCTATTTTGGCAAAATGGTCACTTAGAACCTGAGTTACAAGATGAACAGCCAATTA
ATATATTAGGATCTGTTCAAATCAACGAATGGAATGGTAATCAATCACCGCAATTAATTATTCAAGATATTGCGATGAAT
GAACAGCAAATATTAGATTATAGAAGTAAGCGAAAAAGTTTACCTTTTACAGAAAATGATGAAAATATTGTCGTGCTTAT
TCATCCTAAAAGTGATAAAGTAAATGCGAATGAATATTATTATGGTGAAGAAATTAAACATCAAACTGATAAAGTAGTAT
TAAGAGATTTACCAACGTCAATGGAAGACTTGTCTAATTCCTTGCAACAACTGCAATTTTCTCAACTTTATATAGTTTTG
CAACATAATCATTCGATTTACTTCGATGGTATACCTAATATGGATATTTTTAAAAAGTGTTATAAAGCATTAATAACTAA
ACAAGAAACAAATATCCAGAAAGAGGGTATGTTATTGTGTCAACATTTAAGTGTGAAACCAGATACACTTAAATTCATGT
TGAAAGTTTTCTTAGATTTAAAATTTGTAACACAAGAAGATGGTTTAATTCGAATCAATCAACAACCTGATAAAAGATCG
ATTGATTCCAGCAAAGTATATCAATTAAGACAACAACGTATGGATGTTGAAAAGCAATTATTATATCAAGATTTTTCAGA
AATAAAAAATTGGATAAAGTCACAATTGTCGTGA

Domains

Predicted by InterProScan.

DHHA1 domain (DHHA1)

(312-436)

RecJ OB domain (RecJ_OB)

(452-556)

Single-strand DNA-specific exonuclease, C terminal domain (ssDNA-exonuc_C)

(563-752)

DHH family (DHH)

(81-224)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recJ Bacillus subtilis subsp. subtilis str. 168

38.015

100

0.39
  recJ Streptococcus pneumoniae strain D39V

38.158

100

0.383