Detailed information    

insolico Bioinformatically predicted

Overview

Name   recQ   Type   Machinery gene
Locus tag   U14A_RS21225 Genome accession   NZ_CP035516
Coordinates   4258815..4260644 (+) Length   609 a.a.
NCBI ID   WP_001442069.1    Uniprot ID   -
Organism   Escherichia coli strain U14A     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 4253815..4265644
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  U14A_RS27295 ysgD 4254176..4254232 (+) 57 WP_211180520.1 protein YsgD -
  U14A_RS21195 (U14A_04085) corA 4254384..4255334 (+) 951 WP_000947159.1 magnesium/cobalt transporter CorA -
  U14A_RS21200 (U14A_04086) - 4255377..4255757 (-) 381 WP_000031247.1 DUF2628 domain-containing protein -
  U14A_RS21205 (U14A_04087) - 4255772..4256152 (-) 381 WP_001532065.1 hypothetical protein -
  U14A_RS21210 (U14A_04088) rarD 4256247..4257137 (-) 891 WP_000339102.1 EamA family transporter RarD -
  U14A_RS21215 (U14A_04089) yigI 4257189..4257656 (-) 468 WP_001277142.1 acyl-CoA thioesterase YigI -
  U14A_RS21220 (U14A_04090) pldA 4257821..4258690 (+) 870 WP_001259692.1 phospholipase A -
  U14A_RS21225 (U14A_04091) recQ 4258815..4260644 (+) 1830 WP_001442069.1 ATP-dependent DNA helicase RecQ Machinery gene
  U14A_RS21230 (U14A_04092) rhtC 4260709..4261329 (+) 621 WP_000928838.1 threonine export protein RhtC -
  U14A_RS21235 (U14A_04093) - 4261367..4262227 (-) 861 WP_000133649.1 Rpn family recombination-promoting nuclease/putative transposase -
  U14A_RS21240 (U14A_04094) rhtB 4262386..4263006 (-) 621 WP_000171710.1 homoserine/homoserine lactone efflux protein -
  U14A_RS21245 (U14A_04095) pldB 4263117..4264139 (+) 1023 WP_000487669.1 lysophospholipase L2 -
  U14A_RS21250 (U14A_04096) yigL 4264147..4264947 (+) 801 WP_000285360.1 sugar/pyridoxal phosphate phosphatase YigL -

Sequence

Protein


Download         Length: 609 a.a.        Molecular weight: 68294.40 Da        Isoelectric Point: 7.0905

>NTDB_id=304022 U14A_RS21225 WP_001442069.1 4258815..4260644(+) (recQ) [Escherichia coli strain U14A]
MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCLVVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKD
QVDQLQANGVAAACLNSTQTREQQLEVMTGCRTGQISLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLMEKFKPLDQLMRYVQEQRGKSGI
IYCNSRAKVEDTAARLQSKGISAAAYHAGLENNVRADVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESY
YQETGRAGRDGLPAEAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEGRQEPCGNC
DICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDYGHDKLKVYGMGRDKSHEHWVSVIRQLIHLG
LVTQNIAQHSALQLTEAARPVLRGESSLQLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFND
ATLIEMAEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE

Nucleotide


Download         Length: 1830 bp        

>NTDB_id=304022 U14A_RS21225 WP_001442069.1 4258815..4260644(+) (recQ) [Escherichia coli strain U14A]
GTGGCGCAGGCGGAAGTGTTGAATCTGGAGTCCGGAGCTAAACAGGTTTTACAAGAAACCTTTGGCTACCAACAGTTTCG
CCCCGGCCAGGAAGAGATTATCGACACCGTACTTTCCGGTCGCGATTGCCTGGTCGTCATGCCCACTGGTGGCGGAAAAT
CCCTTTGCTATCAAATCCCTGCCTTATTGCTAAACGGCCTTACCGTGGTTGTCTCACCGCTGATTTCGTTGATGAAAGAT
CAGGTAGATCAACTGCAAGCCAACGGCGTGGCGGCGGCGTGCCTTAACTCGACGCAAACCCGCGAGCAGCAACTTGAAGT
GATGACAGGCTGTCGCACCGGGCAAATCAGCCTGCTTTATATCGCGCCGGAACGCCTGATGCTGGATAACTTTCTTGAGC
ATCTGGCGCACTGGAATCCGGTGTTATTAGCCGTTGATGAGGCGCACTGTATCTCCCAATGGGGTCACGATTTCCGCCCG
GAATACGCTGCCCTCGGTCAGCTGCGTCAGCGTTTTCCGACGCTGCCGTTTATGGCGCTGACTGCCACAGCCGATGACAC
CACGCGCCAGGATATCGTGCGCCTGCTGGGGCTGAACGATCCGCTGATTCAAATCAGCAGTTTTGACCGTCCGAATATTC
GCTACATGCTGATGGAGAAATTCAAACCGCTCGATCAGTTGATGCGTTATGTACAGGAACAACGCGGTAAGTCGGGGATT
ATTTACTGTAATAGTCGTGCGAAAGTAGAAGACACCGCTGCGCGCCTGCAAAGCAAGGGAATTAGCGCGGCGGCCTATCA
TGCCGGGCTGGAAAATAATGTTCGCGCTGATGTGCAGGAAAAATTCCAGCGCGATGACCTGCAAATTGTGGTGGCGACGG
TGGCGTTCGGCATGGGCATCAACAAGCCTAACGTTCGCTTCGTGGTGCATTTTGATATTCCGCGCAATATCGAATCCTAT
TATCAGGAAACTGGACGCGCCGGGCGTGATGGCCTGCCCGCGGAAGCGATGCTGTTTTACGATCCGGCTGATATGGCGTG
GCTGCGCCGTTGTCTGGAAGAGAAGCCGCAGGGGCAGTTGCAGGATATCGAACGTCATAAACTCAATGCGATGGGCGCGT
TTGCTGAAGCGCAAACTTGCCGTCGTTTGGTATTGCTGAACTATTTTGGCGAAGGGCGTCAGGAGCCGTGCGGGAACTGC
GATATCTGCCTCGATCCGCCAAAACAGTACGATGGTTCAACCGATGCGCAGATTGCCCTTTCCACCATTGGTCGTGTGAA
TCAGCGGTTTGGGATGGGTTATGTGGTGGAAGTGATTCGCGGTGCTAATAATCAGCGTATCCGCGACTATGGTCATGACA
AACTGAAAGTCTATGGCATGGGCCGTGATAAAAGCCATGAACATTGGGTGAGCGTGATCCGCCAGCTGATTCACCTCGGC
CTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCTGCGCGTCCGGTGCTGCGCGGCGAATCCTC
TTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAACTATGATCGCA
AACTGTTCGCCAAATTACGCAAACTGCGTAAATCGATTGCCGATGAAAGCAATGTCCCGCCGTACGTGGTGTTTAACGAC
GCTACCTTGATTGAGATGGCTGAACAGATGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGTTGGTATGCGCAA
GCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTCGATGGTGACGACGAAGAGTAG

Domains

Predicted by InterProScan.

DEAD/DEAH box helicase (DEAD)

(28-187)

RecQ zinc-binding (RecQ_Zn_bind)

(343-404)

HRDC domain (HRDC)

(533-599)

Helicase conserved C-terminal domain (Helicase_C)

(223-330)

RQC domain (RQC)

(406-514)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recQ Bacillus subtilis subsp. subtilis str. 168

40.203

97.209

0.391