Detailed information NTG0303952

Bioinformatically predicted

Overview

• Name: pepF
• Type: Regulator
• Locus tag: SAC12_RS07880
• Genome accession: NZ_CP031185
• Coordinates: 1565720..1567525 (-)
• Length: 601 a.a.
• NCBI ID: WP_015473911.1
• Uniprot ID: M5AFG0
• Organism: Levilactobacillus brevis strain SA-C12
• Function: degradation of XIP; competence shut-off (predicted from homology) Competence regulation

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type	MGE coordinates	Gene coordinates	Relative position	Distance (bp)
ICE	1555141..1577431	1565720..1567525	within	0

Gene organization within MGE regions

Location: 1555141..1577431

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
SAC12_RS07825 (SAC12_1533)	asp1	1556607..1558130 (-)	1524	WP_011668190.1	accessory Sec system glycosyltransferase Asp1	-
SAC12_RS07830 (SAC12_1534)	-	1558164..1558370 (-)	207	WP_015473902.1	hypothetical protein	-
SAC12_RS07835 (SAC12_1535)	-	1558386..1558664 (-)	279	WP_176552935.1	hypothetical protein	-
SAC12_RS07840 (SAC12_1536)	-	1558803..1559159 (-)	357	WP_021741809.1	hypothetical protein	-
SAC12_RS07845 (SAC12_1537)	-	1559191..1559580 (-)	390	WP_011668194.1	hypothetical protein	-
SAC12_RS07850 (SAC12_1538)	-	1560041..1560964 (+)	924	WP_086231761.1	IS30 family transposase	-
SAC12_RS07855 (SAC12_1539)	-	1561332..1562228 (-)	897	WP_087609511.1	RluA family pseudouridine synthase	-
SAC12_RS07860 (SAC12_1540)	-	1562225..1563028 (-)	804	WP_021740943.1	NAD kinase	-
SAC12_RS07865 (SAC12_1541)	-	1563030..1563689 (-)	660	WP_011668198.1	GTP pyrophosphokinase family protein	-
SAC12_RS07870 (SAC12_1542)	-	1564003..1564641 (+)	639	WP_011668199.1	ClpXP adapter SpxH family protein	-
SAC12_RS07875 (SAC12_1543)	-	1564803..1565666 (+)	864	WP_139560133.1	DegV family protein	-
SAC12_RS07880 (SAC12_1544)	pepF	1565720..1567525 (-)	1806	WP_015473911.1	oligoendopeptidase F	Regulator
SAC12_RS07885 (SAC12_1545)	-	1567592..1568686 (-)	1095	WP_015473912.1	competence protein CoiA	-
SAC12_RS07890 (SAC12_1546)	trmB	1568813..1569460 (-)	648	WP_015473913.1	tRNA (guanosine(46)-N7)-methyltransferase TrmB	-
SAC12_RS07895 (SAC12_1547)	-	1569526..1570317 (-)	792	WP_011668204.1	phosphotransferase family protein	-
SAC12_RS07900 (SAC12_1548)	-	1570379..1571605 (-)	1227	WP_011668205.1	ABC transporter permease	-
SAC12_RS07905 (SAC12_1549)	-	1571602..1572336 (-)	735	WP_011668206.1	ABC transporter ATP-binding protein	-
SAC12_RS07910 (SAC12_1550)	-	1572546..1573004 (+)	459	WP_011668207.1	HIT family protein	-
SAC12_RS07915 (SAC12_1551)	-	1573007..1573327 (+)	321	WP_015473917.1	hypothetical protein	-
SAC12_RS07920 (SAC12_1552)	-	1573440..1574357 (+)	918	WP_011668209.1	peptidylprolyl isomerase PrsA	-
SAC12_RS07925 (SAC12_1553)	-	1574421..1575395 (-)	975	WP_015473919.1	3'-5' exoribonuclease YhaM family protein	-

Sequence

Protein

• Length: 601 a.a.
• Molecular weight: 67135.10 Da
• Isoelectric Point: 4.7804

>NTDB_id=303952 SAC12_RS07880 WP_015473911.1 1565720..1567525(-) (pepF) [Levilactobacillus brevis strain SA-C12]
MKQIPKRSEVPTALTWDLTTIFPDEAAFKAAIAAIKTQTTVVAGLKGQLAQSGAALYRVTTAVFDLNRQLERVYVYASLN
NDQDTGNAAAQALMGQAESLVATVGAATAWFEPEVLALSADQLQTLIDNDPRLADYQHVFDVLGQQRAHTLSVAEEKLLA
GASDIFGASAKTYSVLSDADLKFPVVQDEAGNDVRLSEGLYGVLLQSTTPRVRQQAFEALYSVYQQFRHTFAATLASEVK
THNFSAETRHYASARAAAMSRNNVPAVVYDTLVETVNDHLDSLHRYVNLRKEILDLPQLHMYDLYTPITGEPSLKYTYQE
AQEMALKALAVLGPDYTANVQKMFDGRAIDVVENQGKRTGAYSGGMYDTKPYILLNWQDSLESLFTLVHEMGHSMHSHYT
RTNQPYQYGDYSIFVAEIASTTNENLLTDYLLKTQTDPKVRAYVLNHYLDGFKGTVYRQTQFAEFEDYIHQQDAAGETLT
ADFMSDFYGKLNQRYYGDGVISDPQIADEWTRIPHFYYDYYVYQYATGFAAATTLSQRILSGDEAKRDAYLAYLKAGSSA
LPLDVMKQAGVDMTQPGYLQTAFATFDERLAEFTQLAHELN

Nucleotide

• Length: 1806 bp

>NTDB_id=303952 SAC12_RS07880 WP_015473911.1 1565720..1567525(-) (pepF) [Levilactobacillus brevis strain SA-C12]
GTGAAACAAATTCCGAAACGATCAGAGGTTCCCACGGCACTGACTTGGGACCTGACAACTATTTTTCCCGACGAGGCAGC
TTTTAAGGCCGCAATTGCTGCAATTAAAACGCAAACAACGGTCGTGGCCGGTTTGAAGGGACAATTGGCCCAAAGTGGGG
CCGCGTTATATCGTGTCACGACGGCGGTATTTGATTTGAATCGCCAGTTGGAGCGGGTATATGTCTATGCGTCTTTAAAC
AATGATCAGGATACCGGCAATGCTGCAGCCCAGGCATTAATGGGGCAGGCCGAGAGCTTGGTGGCAACTGTGGGTGCCGC
CACGGCTTGGTTTGAACCTGAGGTATTGGCCTTGTCAGCGGATCAGCTGCAAACGTTGATTGATAACGACCCCCGGCTAG
CAGATTATCAGCATGTGTTTGACGTATTGGGCCAGCAGCGGGCACATACGCTGTCAGTGGCGGAAGAAAAATTGCTAGCG
GGTGCTAGTGATATCTTTGGTGCATCTGCTAAAACTTACAGCGTGCTGAGTGATGCTGATCTAAAGTTTCCAGTTGTTCA
AGACGAAGCCGGCAATGATGTGCGACTGTCAGAAGGACTGTATGGTGTTTTGCTACAATCCACAACACCACGGGTGCGGC
AGCAGGCTTTTGAAGCATTATATTCGGTTTATCAACAATTCCGACATACCTTTGCTGCAACCTTAGCGAGCGAAGTCAAA
ACGCATAATTTTAGTGCTGAGACGCGTCACTATGCGAGTGCCAGAGCGGCAGCGATGAGTCGCAACAATGTGCCAGCGGT
GGTCTATGATACCTTGGTGGAAACAGTCAATGATCATTTAGACTCACTGCATCGCTATGTTAATTTGCGCAAAGAGATTT
TGGACTTGCCACAACTGCACATGTACGACCTGTATACGCCAATTACGGGTGAACCCAGCTTGAAATATACCTACCAAGAA
GCACAAGAGATGGCGTTAAAGGCACTAGCAGTCTTGGGACCGGATTACACTGCCAATGTTCAAAAGATGTTTGATGGCCG
GGCAATTGATGTGGTTGAAAATCAGGGTAAACGGACGGGCGCTTACTCAGGCGGGATGTATGATACTAAACCGTATATTT
TGTTGAATTGGCAAGATAGCTTGGAAAGCTTGTTCACGTTGGTTCATGAGATGGGACACAGCATGCATAGCCATTACACT
CGGACAAATCAACCGTACCAGTATGGTGATTATTCAATCTTTGTTGCGGAAATTGCCTCGACAACGAATGAAAACTTGCT
GACGGACTACCTGTTGAAAACCCAGACTGACCCCAAGGTGCGAGCTTATGTATTAAATCATTATCTGGATGGCTTTAAAG
GCACGGTATACCGCCAGACGCAGTTCGCAGAATTTGAGGACTACATTCACCAACAGGATGCCGCTGGCGAAACTCTGACA
GCTGACTTCATGAGTGATTTCTACGGCAAACTTAATCAGCGTTATTACGGTGATGGCGTGATTTCCGATCCACAAATCGC
TGATGAATGGACCCGAATTCCACATTTCTACTATGACTACTATGTGTACCAGTACGCAACCGGGTTTGCGGCGGCCACGA
CGTTATCGCAACGAATTTTGAGTGGCGACGAGGCAAAGCGGGATGCCTACTTGGCCTATCTAAAGGCGGGAAGTTCTGCC
TTACCACTTGATGTGATGAAACAAGCGGGCGTCGATATGACGCAACCGGGCTATTTGCAAACGGCTTTTGCAACGTTCGA
CGAGCGCTTGGCAGAATTCACGCAATTGGCGCATGAATTAAACTAA

Domains

Predicted by InterproScan.

• Oligopeptidase F (Peptidase_M3_N) (114-183)

• Peptidase family M3 (Peptidase_M3) (204-584)

3D structure

• AlphaFold DB: M5AFG0

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pepF	Streptococcus salivarius strain HSISS4	49.831	98.17	0.489