Detailed information    

insolico Bioinformatically predicted

Overview

Name   radC   Type   Machinery gene
Locus tag   EQO00_RS00490 Genome accession   NZ_CP035498
Coordinates   94802..95470 (+) Length   222 a.a.
NCBI ID   WP_001297375.1    Uniprot ID   P65957
Organism   Escherichia coli strain 04-00955     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 89802..100470
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EQO00_RS00460 (EQO00_00460) yicC 89821..90684 (-) 864 WP_000621332.1 YicC/YloC family endoribonuclease -
  EQO00_RS00465 (EQO00_00465) rph 90811..91527 (+) 717 WP_001247094.1 ribonuclease PH -
  EQO00_RS00470 (EQO00_00470) pyrE 91593..92234 (+) 642 WP_000806177.1 orotate phosphoribosyltransferase -
  EQO00_RS00475 (EQO00_00475) slmA 92271..92867 (-) 597 WP_000818603.1 nucleoid occlusion factor SlmA -
  EQO00_RS00480 (EQO00_00480) dut 92974..93432 (-) 459 WP_000976070.1 dUTP diphosphatase -
  EQO00_RS00485 (EQO00_00485) coaBC 93410..94630 (-) 1221 WP_000050139.1 bifunctional phosphopantothenoylcysteine decarboxylase/phosphopantothenate--cysteine ligase CoaBC -
  EQO00_RS00490 (EQO00_00490) radC 94802..95470 (+) 669 WP_001297375.1 DNA repair protein RadC Machinery gene
  EQO00_RS00495 (EQO00_00495) rpmB 95687..95923 (+) 237 WP_000091955.1 50S ribosomal protein L28 -
  EQO00_RS00500 (EQO00_00500) rpmG 95944..96111 (+) 168 WP_001051798.1 50S ribosomal protein L33 -
  EQO00_RS00505 (EQO00_00505) mutM 96209..97018 (+) 810 WP_001114533.1 bifunctional DNA-formamidopyrimidine glycosylase/DNA-(apurinic or apyrimidinic site) lyase -
  EQO00_RS00510 (EQO00_00510) coaD 97057..97536 (-) 480 WP_001171873.1 pantetheine-phosphate adenylyltransferase -
  EQO00_RS00515 (EQO00_00515) waaA 97544..98821 (-) 1278 WP_000891574.1 lipid IV(A) 3-deoxy-D-manno-octulosonic acid transferase -
  EQO00_RS00520 (EQO00_00520) rfaQ 99234..100292 (+) 1059 WP_000360397.1 lipopolysaccharide core heptosyltransferase RfaQ -

Sequence

Protein


Download         Length: 222 a.a.        Molecular weight: 25258.35 Da        Isoelectric Point: 7.9748

>NTDB_id=303869 EQO00_RS00490 WP_001297375.1 94802..95470(+) (radC) [Escherichia coli strain 04-00955]
MKNNAQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTLAKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQL
KGIAELARRYYNVRMREESPLLSPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAERGWI

Nucleotide


Download         Length: 669 bp        

>NTDB_id=303869 EQO00_RS00490 WP_001297375.1 94802..95470(+) (radC) [Escherichia coli strain 04-00955]
GTGAAAAACAATGCACAGCTGTTGATGCCGCGCGAAAAAATGCTGAAGTTTGGTATTAGCGCCTTAACGGATGTCGAGCT
GTTGGCGCTATTTCTGCGTACCGGAACGCGCGGTAAAGATGTATTAACCCTGGCAAAAGAGATGCTGGAGAATTTCGGCT
CTCTTTATGGCTTGTTAACCTCTGAATATGAGCAATTTAGTGGCGTTCATGGAATTGGCGTGGCGAAATTCGCCCAGTTA
AAGGGGATTGCCGAACTGGCGCGGCGTTACTACAACGTACGGATGCGTGAAGAAAGCCCTTTACTCAGCCCGGAGATGAC
GAGGGAATTTTTACAAAGCCAGCTCACGGGCGAGGAGCGGGAGATCTTTATGGTGATCTTTCTCGACTCCCAACACAGAG
TTATAACGCATAGCCGTCTTTTTTCCGGCACGCTAAACCATGTTGAAGTCCATCCTCGGGAAATTATCCGCGAAGCGATA
AAAATAAACGCCTCGGCGCTGATCCTTGCGCATAATCACCCTTCGGGTTGTGCTGAACCCAGTAAAGCGGATAAACTCAT
TACTGAACGGATAATAAAGAGTTGTCAGTTCATGGATTTACGCGTGCTCGACCATATCGTGATTGGGCGTGGAGAGTATG
TTTCTTTTGCCGAACGCGGCTGGATTTAA

Domains

Predicted by InterProScan.

RadC-like JAB domain (RadC)

(103-220)

UPF0758 N-terminal (UPF0758_N)

(9-77)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P65957

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radC Haemophilus influenzae Rd KW20

49.55

100

0.496