Detailed information    

insolico Bioinformatically predicted

Overview

Name   recQ   Type   Machinery gene
Locus tag   EUJ32_RS21390 Genome accession   NZ_CP035486
Coordinates   4368700..4370529 (+) Length   609 a.a.
NCBI ID   WP_000035581.1    Uniprot ID   Q3YVE4
Organism   Escherichia coli strain NissleGFP_pZE21     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 4363700..4375529
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EUJ32_RS21365 (EUJ32_21365) - 4364480..4364977 (-) 498 WP_000823790.1 DUF943 family protein -
  EUJ32_RS21370 (EUJ32_21370) - 4364974..4365822 (-) 849 WP_001059676.1 YPO3983 family protein -
  EUJ32_RS21375 (EUJ32_21375) rarD 4366133..4367023 (-) 891 WP_000339101.1 EamA family transporter RarD -
  EUJ32_RS21380 (EUJ32_21380) yigI 4367075..4367542 (-) 468 WP_001277142.1 acyl-CoA thioesterase YigI -
  EUJ32_RS21385 (EUJ32_21385) pldA 4367706..4368575 (+) 870 WP_001259692.1 phospholipase A -
  EUJ32_RS21390 (EUJ32_21390) recQ 4368700..4370529 (+) 1830 WP_000035581.1 ATP-dependent DNA helicase RecQ Machinery gene
  EUJ32_RS21395 (EUJ32_21395) rhtC 4370593..4371213 (+) 621 WP_000928839.1 threonine export protein RhtC -
  EUJ32_RS21400 (EUJ32_21400) rhtB 4371274..4371894 (-) 621 WP_000171710.1 homoserine/homoserine lactone efflux protein -
  EUJ32_RS21405 (EUJ32_21405) pldB 4372005..4373027 (+) 1023 WP_000487654.1 lysophospholipase L2 -
  EUJ32_RS21410 (EUJ32_21410) yigL 4373035..4373835 (+) 801 WP_000285366.1 sugar/pyridoxal phosphate phosphatase YigL -
  EUJ32_RS21415 (EUJ32_21415) bioP 4373911..4374810 (+) 900 WP_001196237.1 biotin transporter -

Sequence

Protein


Download         Length: 609 a.a.        Molecular weight: 68363.51 Da        Isoelectric Point: 7.2544

>NTDB_id=303852 EUJ32_RS21390 WP_000035581.1 4368700..4370529(+) (recQ) [Escherichia coli strain NissleGFP_pZE21]
MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCLVVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKD
QVDQLQANGVAAACLNSTQTREQQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLMEKFKPLDQLMRYVQEQRGKSGI
IYCNSRAKVEDTAARLQSKGISAAAYHAGLENNVRADVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESY
YQETGRAGRDGLPAEAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEGRQEPCGNC
DICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDYGHDKLKVYGMGRDKSHEHWVSVIRQLIHLG
LVTQNIAQHSALQLTEAARPVLRGESSLQLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFND
ATLIEMAEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE

Nucleotide


Download         Length: 1830 bp        

>NTDB_id=303852 EUJ32_RS21390 WP_000035581.1 4368700..4370529(+) (recQ) [Escherichia coli strain NissleGFP_pZE21]
GTGGCGCAGGCGGAAGTGTTGAATCTGGAGTCCGGAGCTAAACAGGTTTTACAAGAAACCTTTGGCTACCAACAGTTTCG
CCCCGGCCAGGAAGAGATTATCGACACCGTACTTTCCGGTCGCGATTGCCTGGTCGTCATGCCCACTGGTGGCGGAAAAT
CCCTTTGCTATCAAATCCCTGCCTTATTGCTAAACGGCCTTACCGTGGTTGTCTCACCGCTGATTTCGTTGATGAAAGAT
CAGGTAGATCAACTGCAAGCCAACGGCGTGGCGGCGGCGTGCCTTAACTCGACGCAAACCCGCGAGCAGCAGCTTGAAGT
AATGACAGGCTGCCGCACCGGGCAAATCCGCCTGCTTTATATCGCGCCGGAACGCCTGATGCTGGATAACTTTCTTGAGC
ATCTGGCGCACTGGAATCCGGTGTTATTAGCCGTTGATGAAGCGCACTGTATCTCCCAATGGGGTCACGATTTCCGCCCG
GAATACGCTGCCCTCGGTCAGCTGCGTCAGCGTTTTCCGACGCTCCCGTTTATGGCGCTGACTGCCACAGCCGATGACAC
CACGCGCCAGGATATTGTGCGCCTGCTGGGGCTGAACGATCCGCTAATTCAAATCAGCAGTTTTGACCGTCCGAATATTC
GCTACATGCTGATGGAGAAATTCAAACCGCTCGATCAGTTGATGCGCTACGTACAGGAACAACGCGGTAAGTCGGGAATT
ATCTACTGCAACAGCCGCGCAAAAGTAGAAGACACCGCTGCACGCCTGCAAAGCAAGGGAATTAGCGCGGCGGCCTATCA
TGCCGGGCTGGAAAATAATGTTCGCGCTGATGTGCAGGAAAAATTCCAGCGCGATGACCTGCAAATTGTGGTGGCGACGG
TGGCGTTCGGCATGGGCATCAACAAGCCTAACGTTCGCTTCGTGGTGCATTTTGATATTCCGCGCAATATCGAATCCTAT
TATCAGGAAACTGGACGCGCCGGGCGTGATGGCCTGCCCGCGGAAGCGATGCTGTTTTACGATCCGGCTGATATGGCGTG
GCTGCGCCGTTGTCTGGAAGAGAAGCCGCAGGGGCAGTTGCAGGATATCGAACGTCATAAACTCAATGCGATGGGCGCGT
TTGCCGAAGCGCAAACTTGTCGTCGTTTGGTATTGCTGAACTATTTTGGCGAAGGGCGTCAGGAGCCGTGCGGGAACTGC
GATATCTGCCTCGATCCGCCGAAACAGTACGACGGTTCAACCGATGCTCAGATTGCCCTTTCCACCATTGGTCGTGTGAA
TCAGCGGTTTGGGATGGGTTATGTGGTGGAAGTGATTCGCGGTGCTAATAACCAGCGTATCCGCGACTATGGTCATGACA
AACTGAAAGTCTATGGCATGGGCCGTGATAAAAGCCATGAACATTGGGTGAGCGTGATCCGCCAGCTGATTCACCTCGGC
CTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCTGCGCGTCCGGTGCTGCGCGGCGAATCCTC
TTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAACTATGATCGCA
AACTGTTCGCCAAATTACGCAAACTGCGTAAATCGATTGCCGATGAAAGCAATGTCCCGCCGTACGTGGTGTTTAACGAC
GCAACCTTGATTGAGATGGCGGAGCAGATGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGTTGGTATGCGCAA
GCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTCGATGGTGACGACGAAGAGTAG

Domains

Predicted by InterProScan.

DEAD/DEAH box helicase (DEAD)

(28-187)

RecQ zinc-binding (RecQ_Zn_bind)

(343-404)

Helicase conserved C-terminal domain (Helicase_C)

(223-330)

HRDC domain (HRDC)

(533-599)

RQC domain (RQC)

(406-514)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q3YVE4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recQ Bacillus subtilis subsp. subtilis str. 168

40.203

97.209

0.391

Multiple sequence alignment