Detailed information    

insolico Bioinformatically predicted

Overview

Name   radC   Type   Machinery gene
Locus tag   EUJ32_RS20320 Genome accession   NZ_CP035486
Coordinates   4149179..4149847 (-) Length   222 a.a.
NCBI ID   WP_001297375.1    Uniprot ID   P65957
Organism   Escherichia coli strain NissleGFP_pZE21     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 4144179..4154847
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EUJ32_RS20290 (EUJ32_20290) rfaQ 4144357..4145415 (-) 1059 WP_000360415.1 lipopolysaccharide core heptosyltransferase RfaQ -
  EUJ32_RS20295 (EUJ32_20295) waaA 4145828..4147105 (+) 1278 WP_000891564.1 lipid IV(A) 3-deoxy-D-manno-octulosonic acid transferase -
  EUJ32_RS20300 (EUJ32_20300) coaD 4147113..4147592 (+) 480 WP_001171873.1 pantetheine-phosphate adenylyltransferase -
  EUJ32_RS20305 (EUJ32_20305) mutM 4147631..4148440 (-) 810 WP_001114542.1 bifunctional DNA-formamidopyrimidine glycosylase/DNA-(apurinic or apyrimidinic site) lyase -
  EUJ32_RS20310 (EUJ32_20310) rpmG 4148538..4148705 (-) 168 WP_001051798.1 50S ribosomal protein L33 -
  EUJ32_RS20315 (EUJ32_20315) rpmB 4148726..4148962 (-) 237 WP_000091955.1 50S ribosomal protein L28 -
  EUJ32_RS20320 (EUJ32_20320) radC 4149179..4149847 (-) 669 WP_001297375.1 DNA repair protein RadC Machinery gene
  EUJ32_RS20325 (EUJ32_20325) coaBC 4150019..4151239 (+) 1221 WP_000050139.1 bifunctional phosphopantothenoylcysteine decarboxylase/phosphopantothenate--cysteine ligase CoaBC -
  EUJ32_RS20330 (EUJ32_20330) dut 4151217..4151675 (+) 459 WP_000976070.1 dUTP diphosphatase -
  EUJ32_RS20335 (EUJ32_20335) slmA 4151782..4152378 (+) 597 WP_000818603.1 nucleoid occlusion factor SlmA -
  EUJ32_RS20340 (EUJ32_20340) pyrE 4152415..4153056 (-) 642 WP_000806177.1 orotate phosphoribosyltransferase -
  EUJ32_RS20345 (EUJ32_20345) rph 4153122..4153838 (-) 717 WP_001247093.1 ribonuclease PH -
  EUJ32_RS20350 (EUJ32_20350) yicC 4153965..4154828 (+) 864 WP_000621330.1 YicC/YloC family endoribonuclease -

Sequence

Protein


Download         Length: 222 a.a.        Molecular weight: 25258.35 Da        Isoelectric Point: 7.9748

>NTDB_id=303841 EUJ32_RS20320 WP_001297375.1 4149179..4149847(-) (radC) [Escherichia coli strain NissleGFP_pZE21]
MKNNAQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTLAKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQL
KGIAELARRYYNVRMREESPLLSPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAERGWI

Nucleotide


Download         Length: 669 bp        

>NTDB_id=303841 EUJ32_RS20320 WP_001297375.1 4149179..4149847(-) (radC) [Escherichia coli strain NissleGFP_pZE21]
GTGAAAAACAATGCACAGCTGTTGATGCCGCGCGAAAAAATGCTGAAGTTTGGTATTAGCGCCTTAACGGATGTCGAGCT
GCTGGCGCTATTTCTGCGTACCGGAACGCGCGGTAAAGATGTATTAACCCTGGCAAAAGAGATGCTGGAGAATTTCGGCT
CTCTTTATGGCTTGTTAACCTCTGAATATGAGCAATTTAGTGGCGTTCATGGAATTGGCGTGGCGAAATTCGCCCAGTTA
AAGGGGATTGCCGAACTGGCGCGGCGTTACTACAACGTACGGATGCGTGAAGAAAGCCCTTTACTCAGCCCGGAGATGAC
GAGGGAATTTTTACAAAGCCAGCTCACGGGCGAGGAGCGGGAGATCTTTATGGTGATCTTTCTCGACTCCCAACACAGGG
TTATAACGCATAGCCGTCTTTTTTCCGGCACGCTAAACCATGTTGAAGTCCATCCTCGGGAAATTATCCGCGAAGCGATA
AAAATAAACGCCTCGGCGCTGATCCTTGCGCATAATCACCCTTCGGGTTGTGCTGAACCCAGTAAAGCGGATAAACTCAT
TACTGAACGGATAATAAAGAGTTGTCAGTTCATGGATTTACGCGTGCTCGACCATATCGTGATTGGGCGTGGAGAGTATG
TTTCTTTTGCCGAACGCGGCTGGATTTAA

Domains

Predicted by InterProScan.

RadC-like JAB domain (RadC)

(103-220)

UPF0758 N-terminal (UPF0758_N)

(9-77)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P65957

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radC Haemophilus influenzae Rd KW20

49.55

100

0.496