Detailed information    

insolico Bioinformatically predicted

Overview

Name   crp   Type   Regulator
Locus tag   EUJ32_RS18835 Genome accession   NZ_CP035486
Coordinates   3823798..3824430 (+) Length   210 a.a.
NCBI ID   WP_000242755.1    Uniprot ID   A0A1C4FZU4
Organism   Escherichia coli strain NissleGFP_pZE21     
Function   promote expression of competence genes (predicted from homology)   
Competence regulation

Genomic Context

Location: 3818798..3829430
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EUJ32_RS18810 (EUJ32_18810) yheS 3818967..3820880 (+) 1914 WP_000634808.1 ABC transporter ATP-binding protein -
  EUJ32_RS18815 (EUJ32_18815) yheT 3820880..3821902 (+) 1023 WP_000057387.1 hydrolase -
  EUJ32_RS18820 (EUJ32_18820) yheU 3821896..3822114 (+) 219 WP_000907085.1 YheU family protein -
  EUJ32_RS18825 (EUJ32_18825) prkB 3822168..3823037 (+) 870 WP_001274684.1 phosphoribulokinase -
  EUJ32_RS18830 (EUJ32_18830) yhfA 3823092..3823496 (-) 405 WP_001148908.1 OsmC family protein -
  EUJ32_RS18835 (EUJ32_18835) crp 3823798..3824430 (+) 633 WP_000242755.1 cAMP-activated global transcriptional regulator CRP Regulator
  EUJ32_RS18840 (EUJ32_18840) yhfK 3824481..3826571 (+) 2091 WP_001304921.1 YccS/YhfK family putative transporter -
  EUJ32_RS18845 (EUJ32_18845) argD 3826638..3827858 (-) 1221 WP_000963800.1 bifunctional acetylornithine/succinyldiaminopimelate transaminase -
  EUJ32_RS18850 (EUJ32_18850) pabA 3827944..3828507 (-) 564 WP_000601853.1 aminodeoxychorismate synthase component 2 -
  EUJ32_RS18855 (EUJ32_18855) fic 3828539..3829141 (-) 603 WP_001280638.1 putative adenosine monophosphate-protein transferase Fic -
  EUJ32_RS18860 (EUJ32_18860) yhfG 3829131..3829298 (-) 168 WP_000736858.1 YhfG family protein -

Sequence

Protein


Download         Length: 210 a.a.        Molecular weight: 23640.43 Da        Isoelectric Point: 8.2710

>NTDB_id=303835 EUJ32_RS18835 WP_000242755.1 3823798..3824430(+) (crp) [Escherichia coli strain NissleGFP_pZE21]
MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIVKGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEG
QERSAWVRAKTACEVAEISYKKFRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGTR

Nucleotide


Download         Length: 633 bp        

>NTDB_id=303835 EUJ32_RS18835 WP_000242755.1 3823798..3824430(+) (crp) [Escherichia coli strain NissleGFP_pZE21]
ATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAA
GAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAG
ACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGC
CAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGAT
TCAGGTAAACCCGGACATTCTGATGCGTCTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCA
ACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCGGATGCTATGACTCAC
CCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATCGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCAT
TCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGTTTACGGCACTCGTTAA

Domains

Predicted by InterProScan.

Cyclic nucleotide-binding domain (cNMP_binding)

(23-109)

Crp-like helix-turn-helix domain (HTH_Crp_2)

(142-206)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1C4FZU4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  crp Vibrio cholerae strain A1552

95.714

100

0.957
  crp Haemophilus influenzae Rd KW20

77.941

97.143

0.757
  crp Acinetobacter baumannii D1279779

47.343

98.571

0.467

Multiple sequence alignment