Detailed information NTG0303820

Bioinformatically predicted

Overview

• Name: rpoS
• Type: Regulator
• Locus tag: EUJ32_RS15065
• Genome accession: NZ_CP035486
• Coordinates: 3071015..3072007 (-)
• Length: 330 a.a.
• NCBI ID: WP_000081550.1
• Uniprot ID: A0A370V765
• Organism: Escherichia coli strain NissleGFP_pZE21
• Function: regulation of chitinases (predicted from homology) Competence regulation

Genomic Context

Location: 3066015..3077007

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
EUJ32_RS15045 (EUJ32_15045)	ygbM	3066646..3067422 (+)	777	WP_001136915.1	HPr family phosphocarrier protein	-
EUJ32_RS15050 (EUJ32_15050)	ygbN	3067511..3068875 (+)	1365	WP_000104433.1	GntP family transporter	-
EUJ32_RS15055 (EUJ32_15055)	-	3069018..3070061 (-)	1044	WP_001161573.1	MBL fold metallo-hydrolase	-
EUJ32_RS15060 (EUJ32_15060)	-	3070286..3070837 (+)	552	WP_001179443.1	flavodoxin family protein	-
EUJ32_RS15065 (EUJ32_15065)	rpoS	3071015..3072007 (-)	993	WP_000081550.1	RNA polymerase sigma factor RpoS	Regulator
EUJ32_RS15070 (EUJ32_15070)	nlpD	3072070..3073164 (-)	1095	WP_306163061.1	murein hydrolase activator NlpD	-
EUJ32_RS15075 (EUJ32_15075)	pcm	3073349..3073975 (-)	627	WP_000254701.1	protein-L-isoaspartate O-methyltransferase	-
EUJ32_RS15080 (EUJ32_15080)	surE	3073969..3074730 (-)	762	WP_001295182.1	5'/3'-nucleotidase SurE	-
EUJ32_RS15085 (EUJ32_15085)	truD	3074711..3075760 (-)	1050	WP_000568929.1	tRNA pseudouridine(13) synthase TruD	-
EUJ32_RS15090 (EUJ32_15090)	ispF	3075757..3076236 (-)	480	WP_001219237.1	2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase	-
EUJ32_RS15095 (EUJ32_15095)	ispD	3076236..3076946 (-)	711	WP_000246149.1	2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase	-

Sequence

Protein

• Length: 330 a.a.
• Molecular weight: 37972.84 Da
• Isoelectric Point: 4.5597

>NTDB_id=303820 EUJ32_RS15065 WP_000081550.1 3071015..3072007(-) (rpoS) [Escherichia coli strain NissleGFP_pZE21]
MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEELLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFA
RRALRGDVASRRRMIESNLRLVVKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNERITSVDTPLGGDSEKALLDILAD
EKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLARRFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQ
GLNIEALFRE

Nucleotide

• Length: 993 bp

>NTDB_id=303820 EUJ32_RS15065 WP_000081550.1 3071015..3072007(-) (rpoS) [Escherichia coli strain NissleGFP_pZE21]
ATGAGTCAGAATACGCTGAAAGTTCATGATTTAAATGAAGATGCGGAATTTGATGAGAACGGAGTTGAGGTTTTTGACGA
AAAGGCCTTAGTAGAAGAGGAACCCAGTGATAACGATTTGGCCGAAGAGGAACTGTTATCGCAGGGAGCCACACAGCGTG
TGCTGGACGCGACTCAGCTTTACCTTGGTGAGATTGGTTATTCACCACTGTTAACGGCCGAAGAAGAAGTTTATTTTGCG
CGTCGCGCACTGCGTGGAGATGTTGCCTCTCGCCGCCGGATGATCGAGAGTAACTTGCGTCTGGTGGTAAAAATTGCCCG
CCGTTATGGCAATCGTGGTCTGGCGTTGCTGGACCTGATCGAAGAGGGCAACCTGGGGCTGATCCGCGCGGTAGAGAAGT
TTGACCCGGAACGTGGTTTCCGCTTCTCAACATACGCAACCTGGTGGATTCGCCAGACGATCGAACGGGCGATTATGAAC
CAAACCCGTACTATTCGTTTGCCGATTCACATCGTAAAGGAGCTGAACGTTTACCTGCGAACCGCACGTGAGTTGTCCCA
TAAGCTGGACCACGAACCAAGTGCGGAAGAGATCGCAGAGCAACTGGATAAGCCAGTTGATGACGTCAGCCGTATGCTTC
GTCTTAACGAGCGCATTACCTCGGTAGACACCCCGCTGGGTGGTGATTCCGAAAAAGCGTTGCTGGACATCCTGGCCGAT
GAAAAAGAGAATGGTCCGGAAGATACCACGCAAGATGACGATATGAAGCAGAGCATCGTCAAATGGCTGTTCGAGCTGAA
CGCCAAACAGCGTGAAGTACTGGCACGTCGATTCGGTTTGCTGGGGTACGAAGCGGCAACACTGGAAGATGTAGGTCGTG
AAATTGGCCTCACCCGTGAACGTGTTCGCCAGATTCAGGTTGAAGGCCTGCGCCGTTTGCGCGAAATCCTGCAAACGCAG
GGGCTGAATATCGAAGCGCTGTTCCGTGAGTAA

Domains

Predicted by InterProScan.

• Sigma-70 region 3 (Sigma70_r3) (174-248)

• Sigma-70 region 2 (Sigma70_r2) (94-163)

• Sigma-70 factor, region 1.2 (Sigma70_r1_2) (56-88)

• Sigma-70, region 4 (Sigma70_r4) (262-315)

3D structure

• AlphaFold DB: A0A370V765

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
rpoS	Vibrio cholerae O1 biovar El Tor strain E7946	73.065	97.879	0.715