Detailed information    

insolico Bioinformatically predicted

Overview

Name   sbcB   Type   Machinery gene
Locus tag   EUJ32_RS11645 Genome accession   NZ_CP035486
Coordinates   2331912..2333339 (+) Length   475 a.a.
NCBI ID   WP_000980604.1    Uniprot ID   -
Organism   Escherichia coli strain NissleGFP_pZE21     
Function   promote homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2326912..2338339
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EUJ32_RS11625 (EUJ32_11625) sbmC 2328052..2328525 (-) 474 WP_001105376.1 DNA gyrase inhibitor SbmC -
  EUJ32_RS11630 (EUJ32_11630) - 2328582..2329802 (-) 1221 WP_000343765.1 ISL3-like element ISEc53 family transposase -
  EUJ32_RS11635 (EUJ32_11635) - 2329821..2330339 (-) 519 WP_000115885.1 ClbS/DfsB family four-helix bundle protein -
  EUJ32_RS11640 (EUJ32_11640) dacD 2330537..2331703 (-) 1167 WP_001297905.1 serine-type D-Ala-D-Ala carboxypeptidase DacD -
  EUJ32_RS11645 (EUJ32_11645) sbcB 2331912..2333339 (+) 1428 WP_000980604.1 exodeoxyribonuclease I Machinery gene
  EUJ32_RS11650 (EUJ32_11650) plaP 2333413..2334771 (-) 1359 WP_000019194.1 putrescine/proton symporter PlaP -
  EUJ32_RS11655 (EUJ32_11655) yoeI 2334761..2334823 (-) 63 WP_010723108.1 membrane protein YoeI -
  EUJ32_RS11660 (EUJ32_11660) yeeY 2335038..2335967 (-) 930 WP_000803366.1 LysR substrate-binding domain-containing protein -
  EUJ32_RS11665 (EUJ32_11665) yeeZ 2336013..2336837 (-) 825 WP_000754733.1 SDR family oxidoreductase -
  EUJ32_RS11670 (EUJ32_11670) yoeB 2336920..2337174 (-) 255 WP_000767822.1 type II toxin-antitoxin system mRNA interferase toxin YoeB -
  EUJ32_RS11675 (EUJ32_11675) yefM 2337171..2337422 (-) 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM -
  EUJ32_RS11680 (EUJ32_11680) hisL 2337705..2337755 (+) 51 WP_001364200.1 his operon leader peptide -

Sequence

Protein


Download         Length: 475 a.a.        Molecular weight: 54442.73 Da        Isoelectric Point: 5.4720

>NTDB_id=303807 EUJ32_RS11645 WP_000980604.1 2331912..2333339(+) (sbcB) [Escherichia coli strain NissleGFP_pZE21]
MMNDGKQQSTFLFHDYETFGTHPALDRPAQFAAIRTDSEFNVIGEPEVFYCKPADDYLPQPGAVLITGITPQEARAKGEN
EAAFAARIHSLFTVPKTCILGYNNVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDG
LPSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLMALIDVPQMKPLVHVSGMFGAWR
GNTSWVAPLAWHPENRNAVIMVDLAGDISPLLELDSDTLRERLYTAKADLGDNAAVPVKLVHINKCPVLAQANTLRPEDA
DRLGINRQHCLDNLKILRENPQVREKVVAIFAEAEPFTPSENVDAQLYNGFFSDADRAAMKIVLETEPRNLPALDITFVD
KRIEKLLFNYRARNFPGTLDYAEQQRWLEHRRQVFTPEFLQGYADELQMLAQQYADDKEKVALLKALWQYAEEIV

Nucleotide


Download         Length: 1428 bp        

>NTDB_id=303807 EUJ32_RS11645 WP_000980604.1 2331912..2333339(+) (sbcB) [Escherichia coli strain NissleGFP_pZE21]
ATGATGAATGACGGTAAGCAACAATCTACCTTTTTGTTTCACGATTACGAAACCTTTGGCACGCACCCCGCGTTAGATCG
CCCTGCACAGTTCGCAGCCATTCGCACCGATAGCGAATTCAATGTCATCGGCGAACCCGAAGTCTTTTACTGCAAGCCCG
CGGATGACTATTTACCCCAGCCTGGAGCAGTATTAATTACCGGTATTACCCCGCAGGAAGCTCGGGCGAAAGGAGAAAAC
GAAGCCGCATTTGCCGCCCGTATTCACTCGCTTTTTACCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTT
CGACGACGAAGTCACACGCAACGTTTTTTATCGTAATTTCTACGATCCTTACGCCTGGAGCTGGCAGCATGATAACTCGC
GCTGGGATTTACTGGATGTTATGCGTGCCTGTTATGCCCTGCGCCCGGAAGGAATAAACTGGCCTGAAAATGATGACGGT
CTACCGAGCTTTCGCCTTGAGCATTTAACCAAAGCGAATGGTATTGAACATAGCAACGCCCACGATGCGATGGCTGATGT
GTACGCCACTATTGCGATGGCGAAACTGGTAAAAACGCGTCAGCCACGCCTGTTTGATTATCTCTTTACCCATCGTAATA
AACACAAACTGATGGCGTTGATTGATGTTCCGCAGATGAAACCCCTGGTTCACGTTTCCGGAATGTTTGGGGCATGGCGC
GGCAATACCAGCTGGGTGGCACCGCTGGCGTGGCATCCTGAAAATCGCAATGCCGTAATTATGGTGGATTTGGCAGGAGA
CATTTCGCCATTACTGGAGCTGGATAGCGACACATTGCGCGAGCGTTTATATACCGCAAAAGCCGATCTTGGCGATAACG
CCGCCGTTCCGGTTAAGCTGGTGCATATCAATAAATGTCCGGTACTGGCCCAGGCGAATACGCTACGCCCGGAAGATGCC
GACCGACTGGGAATTAATCGTCAGCATTGCCTCGATAACCTAAAAATTCTGCGTGAAAATCCGCAAGTGCGTGAAAAAGT
GGTGGCGATATTCGCGGAAGCCGAACCGTTTACGCCTTCAGAGAACGTGGATGCACAGCTTTATAATGGCTTTTTCAGTG
ACGCCGATCGTGCAGCAATGAAAATTGTGCTGGAAACCGAGCCGCGTAATTTACCGGCACTGGATATCACTTTTGTTGAT
AAACGAATTGAAAAACTGTTGTTCAATTATCGAGCGCGCAACTTCCCGGGGACGCTGGATTATGCCGAGCAGCAACGCTG
GCTGGAGCACCGCCGCCAGGTCTTCACGCCAGAGTTTTTGCAGGGCTACGCTGATGAATTGCAGATGCTGGCACAACAGT
ATGCCGATGACAAAGAGAAAGTGGCGCTGTTAAAAGCGCTTTGGCAGTACGCGGAAGAGATCGTCTGA

Domains

Predicted by InterProScan.

Exonuclease C-terminal (ExoI_SH3)

(212-472)

Exonuclease (RNase_T)

(13-188)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  sbcB Vibrio cholerae O1 biovar El Tor strain E7946

58.936

98.947

0.583

Multiple sequence alignment