Detailed information    

insolico Bioinformatically predicted

Overview

Name   radC   Type   Machinery gene
Locus tag   U12A_RS22660 Genome accession   NZ_CP035476
Coordinates   4042593..4043261 (-) Length   222 a.a.
NCBI ID   WP_001296533.1    Uniprot ID   B7N279
Organism   Escherichia coli strain U12A     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 4037593..4048261
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  U12A_RS22630 (U12A_03872) rfaQ 4037770..4038828 (-) 1059 WP_000360408.1 lipopolysaccharide core heptosyltransferase RfaQ -
  U12A_RS22635 (U12A_03873) waaA 4039242..4040519 (+) 1278 WP_000891564.1 lipid IV(A) 3-deoxy-D-manno-octulosonic acid transferase -
  U12A_RS22640 (U12A_03874) coaD 4040527..4041006 (+) 480 WP_001171854.1 pantetheine-phosphate adenylyltransferase -
  U12A_RS22645 (U12A_03875) mutM 4041045..4041854 (-) 810 WP_001114533.1 bifunctional DNA-formamidopyrimidine glycosylase/DNA-(apurinic or apyrimidinic site) lyase -
  U12A_RS22650 (U12A_03876) rpmG 4041952..4042119 (-) 168 WP_001051798.1 50S ribosomal protein L33 -
  U12A_RS22655 (U12A_03877) rpmB 4042140..4042376 (-) 237 WP_000091955.1 50S ribosomal protein L28 -
  U12A_RS22660 (U12A_03878) radC 4042593..4043261 (-) 669 WP_001296533.1 DNA repair protein RadC Machinery gene
  U12A_RS22665 (U12A_03879) coaBC 4043433..4044653 (+) 1221 WP_000050112.1 bifunctional phosphopantothenoylcysteine decarboxylase/phosphopantothenate--cysteine ligase CoaBC -
  U12A_RS22670 (U12A_03880) dut 4044631..4045089 (+) 459 WP_000976070.1 dUTP diphosphatase -
  U12A_RS22675 (U12A_03881) slmA 4045196..4045792 (+) 597 WP_000818603.1 nucleoid occlusion factor SlmA -
  U12A_RS22680 (U12A_03882) pyrE 4045829..4046470 (-) 642 WP_000806177.1 orotate phosphoribosyltransferase -
  U12A_RS22685 (U12A_03883) rph 4046536..4047252 (-) 717 WP_001247093.1 ribonuclease PH -
  U12A_RS22690 (U12A_03884) yicC 4047379..4048242 (+) 864 WP_000621332.1 YicC/YloC family endoribonuclease -

Sequence

Protein


Download         Length: 222 a.a.        Molecular weight: 25286.40 Da        Isoelectric Point: 7.9748

>NTDB_id=303666 U12A_RS22660 WP_001296533.1 4042593..4043261(-) (radC) [Escherichia coli strain U12A]
MKNNVQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTLAKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQL
KGIAELARRYYNVRMREESPLLSPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAERGWI

Nucleotide


Download         Length: 669 bp        

>NTDB_id=303666 U12A_RS22660 WP_001296533.1 4042593..4043261(-) (radC) [Escherichia coli strain U12A]
GTGAAAAACAATGTACAGCTGTTGATGCCGCGCGAAAAAATGCTGAAGTTTGGTATTAGCGCCTTAACGGATGTCGAGCT
GCTGGCGCTATTTCTGCGTACCGGAACGCGCGGTAAAGATGTATTAACCCTGGCAAAAGAGATGCTGGAGAATTTCGGCT
CTCTTTATGGCTTGTTAACCTCTGAATATGAGCAATTTAGTGGCGTTCATGGAATTGGCGTGGCGAAATTCGCCCAGTTA
AAGGGGATTGCCGAACTGGCGCGGCGTTACTACAACGTACGGATGCGTGAAGAAAGCCCTTTACTCAGCCCGGAGATGAC
GAGGGAATTTTTACAAAGCCAGCTCACGGGCGAGGAGCGGGAGATCTTTATGGTGATCTTTCTCGACTCCCAACACAGGG
TTATAACGCATAGCCGTCTTTTTTCCGGCACGCTAAACCATGTTGAAGTCCATCCTCGGGAAATTATCCGCGAAGCGATA
AAAATAAACGCCTCGGCGCTGATCCTTGCGCATAATCACCCTTCGGGTTGTGCTGAACCCAGTAAAGCGGATAAACTCAT
TACTGAACGGATAATAAAGAGTTGTCAGTTCATGGATTTACGCGTGCTCGACCATATCGTGATTGGGCGTGGAGAGTATG
TTTCTTTTGCCGAACGCGGCTGGATTTAA

Domains

Predicted by InterProScan.

UPF0758 N-terminal (UPF0758_N)

(10-77)

RadC-like JAB domain (RadC)

(103-220)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B7N279

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radC Haemophilus influenzae Rd KW20

49.55

100

0.496