Detailed information NTG0303647

Bioinformatically predicted

Overview

• Name: rpoS
• Type: Regulator
• Locus tag: U12A_RS17405
• Genome accession: NZ_CP035476
• Coordinates: 2967899..2968891 (-)
• Length: 330 a.a.
• NCBI ID: WP_000081550.1
• Uniprot ID: A0A370V765
• Organism: Escherichia coli strain U12A
• Function: regulation of chitinases (predicted from homology) Competence regulation

Genomic Context

Location: 2962899..2973891

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
U12A_RS17385 (U12A_02853)	ygbM	2963537..2964313 (+)	777	WP_001136914.1	HPr family phosphocarrier protein	-
U12A_RS17390 (U12A_02854)	ygbN	2964402..2965766 (+)	1365	WP_000104433.1	GntP family transporter	-
U12A_RS17395 (U12A_02855)	-	2965909..2966952 (-)	1044	WP_001161583.1	MBL fold metallo-hydrolase	-
U12A_RS17400 (U12A_02856)	-	2967177..2967728 (+)	552	WP_001179444.1	flavodoxin family protein	-
U12A_RS17405 (U12A_02857)	rpoS	2967899..2968891 (-)	993	WP_000081550.1	RNA polymerase sigma factor RpoS	Regulator
U12A_RS17410 (U12A_02858)	nlpD	2968954..2970048 (-)	1095	WP_306278776.1	murein hydrolase activator NlpD	-
U12A_RS17415 (U12A_02859)	pcm	2970233..2970859 (-)	627	WP_000254708.1	protein-L-isoaspartate O-methyltransferase	-
U12A_RS17420 (U12A_02860)	surE	2970853..2971614 (-)	762	WP_001295182.1	5'/3'-nucleotidase SurE	-
U12A_RS17425 (U12A_02861)	truD	2971595..2972644 (-)	1050	WP_000568920.1	tRNA pseudouridine(13) synthase TruD	-
U12A_RS17430 (U12A_02862)	ispF	2972641..2973120 (-)	480	WP_001219237.1	2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase	-
U12A_RS17435 (U12A_02863)	ispD	2973120..2973830 (-)	711	WP_000246149.1	2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase	-

Sequence

Protein

• Length: 330 a.a.
• Molecular weight: 37972.84 Da
• Isoelectric Point: 4.5597

>NTDB_id=303647 U12A_RS17405 WP_000081550.1 2967899..2968891(-) (rpoS) [Escherichia coli strain U12A]
MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEELLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFA
RRALRGDVASRRRMIESNLRLVVKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNERITSVDTPLGGDSEKALLDILAD
EKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLARRFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQ
GLNIEALFRE

Nucleotide

• Length: 993 bp

>NTDB_id=303647 U12A_RS17405 WP_000081550.1 2967899..2968891(-) (rpoS) [Escherichia coli strain U12A]
ATGAGTCAGAATACGCTGAAAGTTCATGATTTAAATGAAGATGCGGAATTTGATGAGAACGGAGTTGAGGTTTTTGACGA
AAAGGCCTTAGTAGAAGAGGAACCCAGTGATAACGATTTGGCCGAAGAGGAACTGTTATCGCAGGGAGCCACACAGCGTG
TGCTGGACGCGACTCAGCTTTACCTTGGTGAGATTGGTTATTCACCACTGTTAACGGCCGAAGAAGAAGTTTATTTTGCG
CGTCGCGCACTGCGTGGAGATGTCGCCTCTCGCCGCCGGATGATCGAGAGTAACTTGCGTCTGGTGGTAAAAATTGCCCG
CCGTTATGGCAATCGTGGTCTGGCGTTGCTGGACCTGATCGAAGAGGGCAACCTGGGGCTGATCCGCGCGGTAGAGAAGT
TTGACCCGGAACGTGGTTTCCGCTTCTCAACATACGCAACCTGGTGGATTCGCCAGACGATCGAACGGGCGATTATGAAC
CAAACCCGTACTATTCGTTTGCCGATTCACATCGTAAAGGAGCTGAACGTTTACCTGCGAACCGCACGTGAGTTGTCCCA
TAAGCTGGACCACGAACCAAGTGCGGAAGAGATCGCAGAGCAACTGGATAAGCCAGTTGATGACGTCAGCCGTATGCTTC
GTCTTAACGAGCGCATTACCTCGGTAGACACCCCGCTGGGTGGTGATTCCGAAAAAGCATTGCTGGACATCCTGGCCGAT
GAAAAAGAGAATGGTCCGGAAGATACCACGCAAGATGACGATATGAAGCAGAGCATCGTCAAATGGCTGTTCGAGCTGAA
CGCCAAACAGCGTGAAGTGCTGGCACGTCGATTCGGTTTGCTGGGGTACGAAGCGGCAACACTGGAAGATGTAGGTCGTG
AAATTGGCCTCACCCGTGAACGTGTTCGCCAGATTCAGGTTGAAGGCCTGCGCCGTTTGCGCGAAATCCTGCAAACGCAG
GGGCTGAATATCGAAGCGCTGTTCCGCGAATAA

Domains

Predicted by InterProScan.

• Sigma-70 region 3 (Sigma70_r3) (174-248)

• Sigma-70 region 2 (Sigma70_r2) (94-163)

• Sigma-70 factor, region 1.2 (Sigma70_r1_2) (56-88)

• Sigma-70, region 4 (Sigma70_r4) (262-315)

3D structure

• AlphaFold DB: A0A370V765

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
rpoS	Vibrio cholerae O1 biovar El Tor strain E7946	73.065	97.879	0.715