Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   DTO96_RS09790 Genome accession   NZ_CP031124
Coordinates   2178282..2179646 (-) Length   454 a.a.
NCBI ID   WP_114563324.1    Uniprot ID   A0A345DCZ0
Organism   Ephemeroptericola cinctiostellae strain F02     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 2176393..2189058 2178282..2179646 within 0


Gene organization within MGE regions


Location: 2176393..2189058
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DTO96_RS09780 (DTO96_101972) - 2176393..2176947 (-) 555 WP_157964398.1 TetR/AcrR family transcriptional regulator -
  DTO96_RS09785 (DTO96_101973) - 2177494..2178030 (+) 537 WP_114563323.1 HPP family protein -
  DTO96_RS09790 (DTO96_101974) radA 2178282..2179646 (-) 1365 WP_114563324.1 DNA repair protein RadA Machinery gene
  DTO96_RS09795 (DTO96_101975) - 2179814..2180581 (-) 768 WP_114563325.1 hypothetical protein -
  DTO96_RS09800 (DTO96_101976) prpB 2180880..2181761 (+) 882 WP_114563326.1 methylisocitrate lyase -
  DTO96_RS09805 (DTO96_101977) prpC 2181845..2183020 (+) 1176 WP_114563327.1 2-methylcitrate synthase -
  DTO96_RS09810 (DTO96_101978) acnD 2183214..2185814 (+) 2601 WP_114563328.1 Fe/S-dependent 2-methylisocitrate dehydratase AcnD -
  DTO96_RS09815 (DTO96_101979) - 2185872..2188478 (+) 2607 WP_225972483.1 AAA family ATPase -
  DTO96_RS09820 (DTO96_101980) - 2188561..2189058 (-) 498 WP_114563329.1 DNA-deoxyinosine glycosylase -

Sequence

Protein


Download         Length: 454 a.a.        Molecular weight: 48150.55 Da        Isoelectric Point: 7.6664

>NTDB_id=303494 DTO96_RS09790 WP_114563324.1 2178282..2179646(-) (radA) [Ephemeroptericola cinctiostellae strain F02]
MATKPKTQYVCNECGGVAGKWAGQCPHCQTWNSLVETVEQKATQNRFASLAPSAPVQSLAQIDAADVPRFSSEVSEFDRV
LGGGLVPGAVILIGGDPGIGKSTLLLQSLAALSKNRNVLYVSGEESGAQIALRAQRLGVSDARMQFQAEIGLEAIMNTLQ
TLKPDVAVIDSIQTIYSGELTAAPGSVSQVRECAAQLTRVAKATGVTLIMVGHVTKEGSLAGPRVLEHIVDTVLYFEGDT
QSSFRLIRAFKNRFGAVNELGVFAMTEKGLKGVTNPSAIFLSQHNSGVAGSCVMVTQEGTRPLLVEIQALVDESHAPAPR
RLSVGLEQNRLAMLLAVLHRHAGVACFDQDVFINAVGGVKITEPAADLAVLLSIVSSMRNKPLPAGLVVFGEIGLAGEIR
PAPRGQERLKEAAKLGFTHALIPKSNAPKQKIDGLKVIAVERIDEAIDKLRAMD

Nucleotide


Download         Length: 1365 bp        

>NTDB_id=303494 DTO96_RS09790 WP_114563324.1 2178282..2179646(-) (radA) [Ephemeroptericola cinctiostellae strain F02]
ATGGCAACTAAGCCCAAAACACAGTATGTATGTAATGAATGCGGCGGCGTCGCTGGCAAATGGGCAGGTCAATGCCCTCA
TTGCCAAACGTGGAACTCGTTGGTTGAAACGGTTGAGCAAAAGGCCACACAAAATCGTTTTGCATCGCTCGCGCCCAGTG
CCCCTGTGCAGTCATTGGCGCAGATTGATGCGGCGGATGTGCCGCGCTTTTCCAGTGAGGTGTCTGAATTTGACCGCGTG
TTGGGCGGAGGTTTGGTACCCGGCGCGGTGATTTTGATTGGTGGAGACCCTGGCATTGGTAAGTCGACGTTGTTGTTGCA
ATCGCTTGCAGCGCTGTCTAAAAATCGAAATGTCTTGTATGTCAGTGGCGAGGAGTCTGGCGCACAAATTGCTTTGCGCG
CGCAGCGGCTGGGCGTGTCTGATGCCCGCATGCAATTTCAAGCGGAAATCGGCTTGGAGGCTATCATGAACACATTGCAA
ACTTTAAAACCCGATGTGGCGGTGATTGACTCGATTCAAACCATTTATTCGGGTGAGTTGACGGCTGCGCCAGGCTCAGT
CTCGCAGGTGCGTGAATGTGCGGCGCAGTTGACTCGCGTGGCAAAAGCCACAGGCGTGACCTTGATCATGGTCGGGCATG
TGACCAAAGAAGGCAGTCTTGCTGGGCCGCGCGTGTTGGAGCACATTGTCGATACGGTGTTGTATTTTGAGGGTGATACG
CAATCGTCATTTCGCTTGATTCGTGCATTTAAAAATCGTTTTGGCGCGGTCAATGAATTGGGTGTGTTCGCCATGACCGA
AAAAGGTTTGAAAGGCGTGACCAATCCGTCGGCGATTTTTTTGTCGCAGCACAACTCAGGTGTGGCGGGCTCGTGCGTGA
TGGTGACGCAAGAGGGCACGCGACCGCTGCTGGTTGAAATTCAAGCCTTGGTGGATGAATCGCATGCACCCGCGCCGCGC
CGCTTGTCGGTGGGGTTGGAACAAAATCGCCTTGCCATGTTATTGGCTGTGCTGCACCGTCATGCGGGTGTGGCATGCTT
TGATCAAGACGTGTTCATCAATGCAGTTGGCGGTGTAAAAATTACCGAGCCTGCGGCTGATCTGGCGGTGTTGTTGTCGA
TTGTTTCTTCCATGCGCAATAAGCCTTTGCCAGCAGGTTTGGTGGTGTTTGGTGAGATCGGTTTGGCGGGTGAAATTCGC
CCTGCACCACGCGGGCAAGAACGTTTGAAAGAGGCGGCGAAATTGGGATTCACCCATGCCCTAATTCCAAAATCCAATGC
GCCAAAACAGAAAATCGATGGTCTGAAAGTGATTGCGGTTGAGCGAATCGATGAAGCGATTGATAAGTTGCGGGCGATGG
ATTGA

Domains

Predicted by InterproScan.

Lon protease (S16) C-terminal proteolytic domain (Lon_C)

(357-449)

AAA domain (AAA_25)

(71-218)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(9-36)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A345DCZ0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

49.111

99.119

0.487
  radA Streptococcus mitis NCTC 12261

47.807

100

0.48
  radA Streptococcus pneumoniae Rx1

47.807

100

0.48
  radA Streptococcus pneumoniae D39

47.807

100

0.48
  radA Streptococcus pneumoniae R6

47.807

100

0.48
  radA Streptococcus pneumoniae TIGR4

47.807

100

0.48
  radA Streptococcus mitis SK321

47.912

100

0.48

Multiple sequence alignment