Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   BCENMC03_RS14195 Genome accession   NC_010508
Coordinates   3122084..3123589 (+) Length   501 a.a.
NCBI ID   WP_012329240.1    Uniprot ID   B1JYN8
Organism   Burkholderia orbicola MC0-3     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3117084..3128589
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BCENMC03_RS14170 (Bcenmc03_2797) kdsD 3118315..3119298 (+) 984 WP_006483136.1 arabinose 5-phosphate isomerase KdsD -
  BCENMC03_RS14175 (Bcenmc03_2798) - 3119298..3119834 (+) 537 WP_012329237.1 KdsC family phosphatase -
  BCENMC03_RS14180 (Bcenmc03_2799) lptC 3119838..3120452 (+) 615 WP_012329238.1 LPS export ABC transporter periplasmic protein LptC -
  BCENMC03_RS14185 (Bcenmc03_2800) lptA 3120473..3121138 (+) 666 WP_012329239.1 lipopolysaccharide transport periplasmic protein LptA -
  BCENMC03_RS14190 (Bcenmc03_2801) lptB 3121135..3121911 (+) 777 WP_006477788.1 LPS export ABC transporter ATP-binding protein -
  BCENMC03_RS14195 (Bcenmc03_2802) rpoN/rpoN1 3122084..3123589 (+) 1506 WP_012329240.1 RNA polymerase factor sigma-54 Machinery gene
  BCENMC03_RS14200 (Bcenmc03_2803) hpf 3123748..3124107 (+) 360 WP_006477786.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  BCENMC03_RS14205 (Bcenmc03_2804) - 3124302..3124814 (+) 513 WP_027806888.1 PTS sugar transporter subunit IIA -
  BCENMC03_RS14210 (Bcenmc03_2805) hprK 3124944..3125912 (+) 969 WP_006483133.1 HPr(Ser) kinase/phosphatase -
  BCENMC03_RS14215 (Bcenmc03_2806) rapZ 3125987..3126895 (+) 909 WP_006477783.1 RNase adapter RapZ -
  BCENMC03_RS14220 (Bcenmc03_2807) - 3126923..3127558 (+) 636 WP_011546241.1 LON peptidase substrate-binding domain-containing protein -

Sequence

Protein


Download         Length: 501 a.a.        Molecular weight: 54753.88 Da        Isoelectric Point: 4.8767

>NTDB_id=30339 BCENMC03_RS14195 WP_012329240.1 3122084..3123589(+) (rpoN/rpoN1) [Burkholderia orbicola MC0-3]
MKASLQLRLSQHLALTPQLQQSIRLLQLSTLELQQEVAMAVAQNPLLENDDEWIASPLRVAADGSLIAQTPPAQNTEPAA
MNGSSTSSERAERDEPAGADEYDGYGSGDGNDGPQWNLDEYGRSSGASDDDDLPPLQVQEAATSLRDHLSAQLRVTQAGP
RDRALVMFLIESLDDDGYLTATLDEVLADLPPELEIDGDELNAALALLHSFDPAGVGARSASECLKLQLLRLDPSPTRTL
ALEIVSQHLELLAARDFTRLRKHLKASDDALRDAHALIRSLEPFPGAAYGKAEADYVVPDIIVKKAGQGWLAELNPEVVP
RLRINNLYANILRNSRGDPSAGSLKQQLQEARWLIKNIQQRFDTILRVAQAIVERQKNFFAHGEIAMRPLVLREIADTLG
LHESTVSRVTTGKYMLTPFGTLEFKYFFGSHVSTDTGGAASSTAIRALIKQLIGAEDPKSPLSDSRIAELLAEQGFVVAR
RTVAKYREALKIPAVNLRKSL

Nucleotide


Download         Length: 1506 bp        

>NTDB_id=30339 BCENMC03_RS14195 WP_012329240.1 3122084..3123589(+) (rpoN/rpoN1) [Burkholderia orbicola MC0-3]
ATGAAAGCCAGCCTTCAACTCCGCCTGTCGCAACATCTTGCGCTGACGCCTCAGCTGCAGCAGTCGATCCGGCTCCTGCA
GTTGTCGACGCTCGAATTGCAGCAGGAAGTCGCGATGGCCGTCGCGCAGAACCCGCTGCTCGAGAACGACGACGAATGGA
TCGCCAGCCCGCTGCGCGTCGCGGCGGACGGATCGCTGATCGCGCAGACGCCGCCCGCACAGAACACCGAGCCGGCCGCG
ATGAACGGCAGCAGCACGTCCAGCGAGCGCGCCGAGCGCGACGAGCCGGCCGGCGCCGACGAATACGACGGCTACGGGTC
CGGTGACGGCAACGACGGCCCGCAGTGGAATCTCGACGAATACGGCCGCTCGAGCGGTGCGTCCGACGACGACGACCTGC
CGCCGCTGCAGGTGCAGGAAGCCGCGACGTCGCTGCGCGATCATCTCTCGGCGCAGTTGCGCGTCACGCAGGCCGGCCCG
CGCGATCGCGCGCTCGTGATGTTCCTGATCGAATCGCTCGACGACGACGGCTACCTGACCGCCACGCTCGACGAAGTGCT
CGCCGACCTGCCGCCCGAACTGGAAATCGACGGCGACGAACTGAATGCGGCGCTCGCGCTGCTGCACAGCTTCGACCCGG
CCGGCGTCGGCGCGCGCTCGGCCTCCGAATGCCTGAAGCTGCAGCTGCTGCGTCTCGATCCGTCCCCCACGCGCACGCTC
GCGCTCGAGATCGTGTCGCAGCATCTCGAACTGCTCGCCGCACGCGACTTCACACGGTTGCGCAAGCACCTGAAGGCGAG
CGACGACGCGCTGCGCGACGCGCACGCGCTGATCCGCTCGCTGGAGCCGTTCCCCGGCGCCGCCTACGGCAAGGCCGAGG
CCGACTACGTCGTGCCCGACATCATCGTGAAGAAGGCCGGCCAGGGATGGCTCGCGGAGCTCAATCCGGAGGTCGTGCCG
CGCCTGCGGATCAACAACCTGTACGCGAACATCCTGCGCAACAGCCGCGGCGATCCGTCGGCCGGATCGTTGAAGCAGCA
GCTGCAGGAAGCGCGCTGGTTGATCAAGAACATCCAGCAGCGTTTCGACACGATCCTGCGTGTCGCGCAGGCTATTGTCG
AGCGTCAGAAGAATTTCTTTGCGCACGGTGAAATTGCCATGCGCCCCTTGGTTTTGCGGGAAATAGCTGATACGCTGGGC
CTACACGAGTCGACTGTCAGCCGTGTGACAACCGGGAAGTACATGCTGACCCCGTTCGGGACGCTTGAATTTAAGTACTT
CTTCGGATCTCACGTCTCGACCGACACCGGTGGCGCCGCCTCGTCGACCGCCATCCGAGCCCTCATCAAGCAACTGATAG
GAGCTGAAGACCCAAAATCGCCACTTTCGGACAGCCGCATTGCCGAGCTGCTGGCAGAACAGGGTTTCGTGGTCGCGCGC
CGCACGGTTGCGAAATATCGCGAAGCCCTCAAGATCCCGGCAGTGAATCTGCGCAAGTCTCTTTAA

Domains

Predicted by InterproScan.

Sigma-54, DNA binding domain (Sigma54_DBD)

(344-499)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(137-328)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B1JYN8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

67.984

100

0.687

Multiple sequence alignment