Detailed information    

insolico Bioinformatically predicted

Overview

Name   ruvB   Type   Machinery gene
Locus tag   VCM66_RS08800 Genome accession   NC_012578
Coordinates   1921286..1922290 (-) Length   334 a.a.
NCBI ID   WP_000568491.1    Uniprot ID   Q9KR02
Organism   Vibrio cholerae M66-2     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1916286..1927290
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VCM66_RS08770 (VCM66_1762) tolQ 1916408..1917091 (-) 684 WP_000123084.1 protein TolQ -
  VCM66_RS08775 (VCM66_1763) ybgC 1917081..1917488 (-) 408 WP_001091290.1 tol-pal system-associated acyl-CoA thioesterase -
  VCM66_RS08780 (VCM66_1764) ybgE 1917657..1917974 (-) 318 WP_001286617.1 cyd operon protein YbgE -
  VCM66_RS08785 (VCM66_1765) cydX 1917967..1918074 (-) 108 WP_000270285.1 cytochrome bd-I oxidase subunit CydX -
  VCM66_RS08790 (VCM66_1766) cydB 1918087..1919223 (-) 1137 WP_000460767.1 cytochrome d ubiquinol oxidase subunit II -
  VCM66_RS08795 (VCM66_1767) cydA 1919241..1920827 (-) 1587 WP_000568056.1 cytochrome ubiquinol oxidase subunit I -
  VCM66_RS08800 (VCM66_1768) ruvB 1921286..1922290 (-) 1005 WP_000568491.1 Holliday junction branch migration DNA helicase RuvB Machinery gene
  VCM66_RS08805 (VCM66_1769) ruvA 1922346..1922960 (-) 615 WP_000580359.1 Holliday junction branch migration protein RuvA Machinery gene
  VCM66_RS08810 (VCM66_1770) ruvC 1923057..1923578 (-) 522 WP_000111361.1 crossover junction endodeoxyribonuclease RuvC -
  VCM66_RS08815 (VCM66_1771) cysS 1923666..1925045 (-) 1380 WP_000913180.1 cysteine--tRNA ligase -
  VCM66_RS08820 (VCM66_1772) - 1925191..1925685 (+) 495 WP_000631302.1 peptidylprolyl isomerase -
  VCM66_RS08825 (VCM66_1773) lpxH 1925726..1926466 (+) 741 WP_000557000.1 UDP-2,3-diacylglucosamine diphosphatase -

Sequence

Protein


Download         Length: 334 a.a.        Molecular weight: 37298.75 Da        Isoelectric Point: 5.0460

>NTDB_id=30339 VCM66_RS08800 WP_000568491.1 1921286..1922290(-) (ruvB) [Vibrio cholerae M66-2]
MIEADRLIAPISNHFKDEEVIDRAIRPKKLADYQGQDHVRDQMEIFIQAAQMRQEALDHLLIFGPPGLGKTTLANIVANE
MGVNIRTTSGPVLEKAGDLAALLTNLEENDVLFIDEIHRLSPMVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLI
GATTRAGSLTSPLRDRFGIVQRLEYYKVADLQHIVQRSAQCLGLSMDSEGALEVARRARGTPRIANRLLRRVRDYAEVKG
DGHICAQTADRALNMLDVDHQGFDYMDRKLLLAIMEKFSGGPVGIDNLAAAIGEEKDTIEDVLEPFLIQQGYLQRTPRGR
IATDRAYLHFGIEK

Nucleotide


Download         Length: 1005 bp        

>NTDB_id=30339 VCM66_RS08800 WP_000568491.1 1921286..1922290(-) (ruvB) [Vibrio cholerae M66-2]
ATGATAGAAGCCGATCGTTTAATTGCTCCAATCAGCAACCACTTCAAAGATGAGGAAGTGATTGATCGAGCGATTCGTCC
CAAAAAGCTTGCCGATTACCAAGGTCAAGATCATGTTCGTGATCAGATGGAAATCTTCATTCAAGCGGCACAAATGCGCC
AAGAAGCCTTAGATCATCTGCTGATTTTCGGGCCTCCGGGCTTGGGTAAAACCACCTTGGCCAACATAGTCGCTAACGAA
ATGGGCGTGAATATTCGTACTACTTCAGGTCCTGTATTGGAAAAAGCGGGAGATTTAGCGGCACTGCTAACCAATCTTGA
AGAAAACGATGTGCTGTTTATTGATGAAATCCATCGTCTGAGCCCAATGGTTGAGGAAGTACTGTATCCCGCTATGGAAG
ATTACCAACTCGATATCATGATTGGTGAAGGCCCAGCTGCACGCTCAATCAAAATCGATCTCCCTCCTTTTACTTTAATT
GGAGCCACCACACGTGCAGGCTCGCTGACTTCACCACTGCGTGACCGATTTGGGATTGTGCAACGATTGGAGTATTACAA
AGTCGCCGATTTACAACACATAGTGCAACGTAGTGCACAGTGTCTCGGTCTTTCGATGGATAGTGAGGGCGCTCTTGAAG
TGGCTCGCCGTGCACGTGGAACCCCACGGATTGCCAATCGCCTGCTGCGCCGGGTACGAGATTATGCCGAAGTAAAAGGG
GATGGGCACATTTGTGCGCAAACCGCTGACAGGGCACTCAATATGCTTGATGTTGACCATCAAGGCTTTGATTACATGGA
TAGAAAGCTGCTGTTAGCAATAATGGAAAAGTTTTCAGGTGGCCCAGTCGGAATCGATAACCTTGCGGCAGCTATTGGCG
AAGAGAAAGATACGATAGAAGATGTTCTTGAGCCATTTCTCATTCAGCAGGGTTATTTACAACGTACACCACGCGGTCGT
ATCGCGACAGATCGCGCTTATTTGCATTTCGGTATTGAAAAATAA

Domains

Predicted by InterProScan.

Holliday junction DNA helicase RuvB P-loop domain (RuvB_N)

(25-183)

RuvB C-terminal winged helix domain (WHD_RuvB)

(261-330)

RuvB AAA lid domain (AAA_lid_4)

(186-259)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q9KR02

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ruvB Bacillus subtilis subsp. subtilis str. 168

61.682

96.108

0.593
  ruvB Streptococcus pneumoniae TIGR4

59.937

94.91

0.569
  ruvB Streptococcus pneumoniae R6

59.937

94.91

0.569
  ruvB Streptococcus pneumoniae D39

59.937

94.91

0.569
  ruvB Synechocystis sp. PCC 6803

55.663

92.515

0.515
  ruvB Helicobacter pylori 26695

54.276

91.018

0.494

Multiple sequence alignment