Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   AGE29_RS14550 Genome accession   NZ_CP031097
Coordinates   2624127..2625485 (+) Length   452 a.a.
NCBI ID   WP_003360170.1    Uniprot ID   -
Organism   Clostridium botulinum strain CFSAN034200     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2619127..2630485
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AGE29_RS14530 (AGE29_14530) - 2619507..2619971 (+) 465 WP_003360166.1 CtsR family transcriptional regulator -
  AGE29_RS14535 (AGE29_14535) - 2619994..2620521 (+) 528 WP_003360167.1 UvrB/UvrC motif-containing protein -
  AGE29_RS14540 (AGE29_14540) - 2620526..2621569 (+) 1044 WP_003360168.1 protein arginine kinase -
  AGE29_RS14545 (AGE29_14545) - 2621571..2624006 (+) 2436 WP_003360169.1 ATP-dependent Clp protease ATP-binding subunit -
  AGE29_RS14550 (AGE29_14550) radA 2624127..2625485 (+) 1359 WP_003360170.1 DNA repair protein RadA Machinery gene
  AGE29_RS14555 (AGE29_14555) disA 2625504..2626565 (+) 1062 WP_003360171.1 DNA integrity scanning diadenylate cyclase DisA -
  AGE29_RS14560 (AGE29_14560) - 2626597..2626995 (-) 399 WP_003357596.1 hypothetical protein -
  AGE29_RS14565 (AGE29_14565) - 2627400..2628500 (+) 1101 WP_003360172.1 PIN/TRAM domain-containing protein -
  AGE29_RS14570 (AGE29_14570) ispD 2628502..2629191 (+) 690 WP_003360173.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -

Sequence

Protein


Download         Length: 452 a.a.        Molecular weight: 49783.77 Da        Isoelectric Point: 8.1297

>NTDB_id=303321 AGE29_RS14550 WP_003360170.1 2624127..2625485(+) (radA) [Clostridium botulinum strain CFSAN034200]
MAKNKIVYICQQCGYESIKWLGKCPGCNSWNSMVEEEKQSTNKTIKNINIKSEPKIISTIKSSEYERLDTGINELNRVLG
GGIVKGSLTLISGSPGIGKSTILLQAANNIATKYGKILYVSGEESEEQIKMRADRLKVISKDIYILSETNVDIIKEHITT
INPKFVIIDSIQTLFKSELSSAPGTVSQVRQCSNDIMLISKSNNIPFFIVAHVTKQGELAGPRVLEHMVDTVLSFEGERT
QEFRILRTVKNRFGTTSEIGVFEMAGEGLLEISNPSAVFLEETEFQREGSVIIGIMEGTRPILIEIQALVSETKAYMPRR
TAVGVDTARLNLILAVLEKKLNIPFYNCDVYVNVVGGLDLTGTFADFGLALALLSSAKSKDIKLPKVLVVGEIGLTGEVR
PVSFCDRIVNEGIKMGFTNIIIPDRNKDKISVKNNVNLIGISSLREGINKVF

Nucleotide


Download         Length: 1359 bp        

>NTDB_id=303321 AGE29_RS14550 WP_003360170.1 2624127..2625485(+) (radA) [Clostridium botulinum strain CFSAN034200]
ATGGCTAAAAATAAAATTGTTTATATATGTCAACAATGTGGATATGAATCGATAAAATGGTTAGGAAAATGTCCAGGATG
CAATTCGTGGAATAGTATGGTAGAGGAAGAAAAGCAATCAACTAATAAGACTATAAAAAATATAAATATTAAATCAGAGC
CTAAAATAATATCTACAATAAAATCAAGTGAATATGAGAGATTAGATACTGGAATAAATGAACTGAATAGAGTCTTAGGC
GGTGGCATAGTTAAGGGATCTTTAACTCTTATATCTGGATCGCCTGGCATAGGGAAGTCTACAATACTTTTACAAGCTGC
TAATAATATTGCTACTAAGTATGGTAAGATTTTATATGTGTCTGGAGAAGAATCAGAAGAGCAGATAAAAATGAGAGCAG
ATAGGCTTAAGGTGATATCAAAAGATATATACATACTTTCAGAGACAAATGTGGATATAATAAAAGAGCATATTACTACA
ATTAATCCTAAATTTGTTATAATAGATTCTATACAGACCCTTTTTAAAAGTGAACTATCTTCAGCTCCAGGTACTGTGTC
TCAAGTTAGGCAATGTTCTAACGATATTATGCTTATAAGTAAAAGCAATAACATACCTTTTTTTATAGTAGCACATGTTA
CAAAACAAGGAGAATTAGCAGGACCTAGGGTATTAGAACATATGGTAGATACGGTTTTATCATTTGAAGGAGAAAGAACC
CAGGAATTTAGGATTTTAAGAACAGTAAAAAATCGTTTTGGAACTACAAGTGAAATTGGAGTTTTTGAAATGGCAGGAGA
AGGGCTATTAGAAATAAGCAATCCCTCTGCTGTATTTTTAGAAGAAACTGAATTTCAAAGGGAAGGTTCTGTAATAATAG
GTATAATGGAAGGGACTAGGCCAATACTTATAGAAATACAAGCATTGGTAAGTGAAACGAAAGCTTATATGCCAAGAAGG
ACGGCAGTGGGAGTAGACACAGCTAGATTGAATTTAATATTAGCTGTGTTAGAAAAAAAATTAAATATACCTTTTTATAA
TTGTGATGTATATGTTAATGTAGTAGGAGGATTAGATTTAACAGGAACTTTTGCTGATTTCGGATTAGCATTAGCTTTAT
TATCTAGTGCTAAATCAAAAGATATAAAGTTACCTAAAGTTTTAGTGGTTGGAGAAATAGGACTTACAGGAGAGGTTCGC
CCCGTAAGTTTTTGTGATAGAATAGTAAATGAAGGTATAAAAATGGGATTTACCAATATAATTATACCTGATAGAAATAA
AGACAAAATTTCTGTTAAAAATAATGTTAACCTAATTGGTATATCTTCTTTAAGAGAAGGTATTAATAAAGTATTTTAA

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(341-426)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

AAA domain (AAA_25)

(60-217)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

49.89

100

0.5
  radA Streptococcus pneumoniae Rx1

48.678

100

0.489
  radA Streptococcus pneumoniae D39

48.678

100

0.489
  radA Streptococcus pneumoniae R6

48.678

100

0.489
  radA Streptococcus pneumoniae TIGR4

48.678

100

0.489
  radA Streptococcus mitis NCTC 12261

48.678

100

0.489
  radA Streptococcus mitis SK321

48.678

100

0.489

Multiple sequence alignment