Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA   Type   Machinery gene
Locus tag   DN407_RS25620 Genome accession   NZ_CP031068
Coordinates   4909356..4910705 (-) Length   449 a.a.
NCBI ID   WP_070184465.1    Uniprot ID   A0A9X5N3V0
Organism   Bacillus sp. JAS24-2     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4904356..4915705
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DN407_RS25585 (DN407_25625) - 4904400..4905095 (-) 696 WP_000466510.1 hypothetical protein -
  DN407_RS25590 (DN407_25630) - 4905082..4905870 (-) 789 WP_000120045.1 hypothetical protein -
  DN407_RS25595 (DN407_25635) - 4905898..4906467 (-) 570 WP_001211057.1 hypothetical protein -
  DN407_RS25600 (DN407_25640) - 4906585..4907058 (-) 474 WP_098286447.1 hypothetical protein -
  DN407_RS25605 (DN407_25645) raiA 4907463..4908005 (-) 543 WP_000671186.1 ribosome-associated translation inhibitor RaiA -
  DN407_RS25610 (DN407_25650) cspC 4908328..4908525 (-) 198 WP_001990088.1 cold shock protein CspC -
  DN407_RS25615 (DN407_25655) - 4908652..4909356 (-) 705 WP_080450914.1 ComF family protein -
  DN407_RS25620 (DN407_25660) comFA 4909356..4910705 (-) 1350 WP_070184465.1 ATP-dependent helicase ComFA Machinery gene
  DN407_RS25625 (DN407_25665) - 4910833..4912260 (-) 1428 WP_141551779.1 C40 family peptidase -
  DN407_RS25630 (DN407_25670) - 4912409..4912723 (-) 315 WP_000400857.1 helix-turn-helix domain-containing protein -
  DN407_RS25635 (DN407_25675) - 4912896..4913738 (-) 843 WP_000684725.1 DegV family protein -
  DN407_RS25640 (DN407_25680) - 4913975..4914610 (+) 636 WP_149216704.1 YigZ family protein -

Sequence

Protein


Download         Length: 449 a.a.        Molecular weight: 50982.88 Da        Isoelectric Point: 9.9654

>NTDB_id=303183 DN407_RS25620 WP_070184465.1 4909356..4910705(-) (comFA) [Bacillus sp. JAS24-2]
MLAGKQLLLEELSSDLQRELNDLKKKGEIVCVQGVKKKNSKYMCQRCGNVDRRLFASFLCKRCSKVCAYCRKCITMGRVS
ECAVLVRGIAERKREKNSNLLQWNGTLSTGQNLAAQGVIEAIKQKESFFIWAVCGAGKTEMLFYGINEALQKGERVCIAT
PRTDVVLELAPRLQEVFPYIKVAALYGGSVDKEKDAVLVVATTHQLLRYYRAFHVMVVDEIDAFPYCADQMLQYAVKQAM
KEKAARIYLTATPDETWKRKLKQGKQKGVIVSGRYHRHPLPVPLFCWCGNWKKNLIHKRIPRVLLQWLQTYLNKKFPIFL
FVPHVRYIEEISLLLKPLNNRIEGVHAEDPGRKEKVAAFKKGEIPLLVTTTILERGVTVKNLQVAVLGAEEEIFSESALV
QIAGRAGRSFEAPYGEVIYFHYGKTEAMVRAKKHIQGMNKNAKEQGLID

Nucleotide


Download         Length: 1350 bp        

>NTDB_id=303183 DN407_RS25620 WP_070184465.1 4909356..4910705(-) (comFA) [Bacillus sp. JAS24-2]
ATGCTAGCGGGGAAACAGTTGCTATTAGAAGAACTTTCTTCAGATTTACAGAGGGAATTAAACGATTTGAAAAAGAAGGG
AGAGATCGTATGTGTACAAGGTGTAAAAAAGAAGAATTCTAAATATATGTGCCAACGCTGCGGAAATGTAGATCGGCGGC
TATTTGCGTCGTTTTTATGTAAAAGGTGCAGTAAAGTGTGCGCATATTGCCGGAAGTGTATAACGATGGGGAGAGTAAGT
GAATGTGCTGTACTTGTTCGCGGGATTGCTGAAAGAAAGAGAGAAAAGAATTCAAATTTGTTACAGTGGAACGGGACGTT
GTCTACTGGCCAGAATTTGGCGGCGCAAGGAGTTATAGAGGCTATTAAGCAAAAAGAATCATTTTTTATTTGGGCTGTAT
GCGGGGCTGGGAAAACAGAGATGTTGTTTTACGGAATTAACGAAGCGCTTCAAAAAGGAGAAAGAGTTTGTATCGCAACG
CCGAGAACGGATGTTGTTCTGGAATTAGCACCGAGATTGCAAGAAGTATTTCCATATATAAAGGTAGCGGCTTTATATGG
AGGGAGTGTGGATAAAGAAAAAGATGCAGTACTAGTCGTTGCGACTACGCATCAATTATTACGTTATTATAGGGCGTTTC
ATGTCATGGTTGTAGATGAGATAGATGCGTTTCCATATTGTGCAGATCAAATGTTACAGTACGCGGTAAAACAAGCGATG
AAAGAAAAAGCTGCGCGTATTTATTTAACTGCGACTCCAGATGAAACGTGGAAGCGAAAACTTAAACAAGGTAAACAAAA
AGGTGTTATTGTTTCTGGACGATATCATCGTCATCCTTTGCCAGTTCCTTTATTTTGTTGGTGCGGGAATTGGAAAAAAA
ACCTCATTCATAAAAGAATTCCTCGAGTTTTACTACAGTGGTTACAAACATACTTAAATAAAAAATTCCCTATTTTTTTA
TTCGTCCCCCATGTGCGATATATAGAAGAAATAAGCTTGTTGTTAAAACCATTAAACAATCGAATTGAAGGTGTACATGC
AGAAGATCCAGGGAGAAAAGAAAAAGTAGCGGCTTTCAAAAAGGGAGAAATCCCATTATTAGTTACAACGACAATTTTAG
AGCGAGGCGTAACGGTGAAAAATTTGCAAGTCGCAGTTTTGGGGGCGGAAGAAGAAATATTTTCAGAAAGTGCGCTCGTA
CAAATTGCGGGCCGAGCAGGGCGGAGCTTTGAAGCACCGTATGGAGAGGTCATTTATTTTCACTATGGCAAGACAGAGGC
GATGGTGCGCGCGAAAAAACATATTCAAGGTATGAATAAAAATGCGAAAGAACAAGGATTGATCGATTAA

Domains

Predicted by InterproScan.

Helicase conserved C-terminal domain (Helicase_C)

(313-408)

Type III restriction enzyme, res subunit (ResIII)

(107-254)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA Bacillus subtilis subsp. subtilis str. 168

51.131

98.441

0.503
  comFA Latilactobacillus sakei subsp. sakei 23K

41.191

89.755

0.37

Multiple sequence alignment