Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   DTO64_RS00195 Genome accession   NZ_CP031027
Coordinates   44060..45430 (+) Length   456 a.a.
NCBI ID   WP_114679326.1    Uniprot ID   -
Organism   Enterococcus faecalis strain TY1 isolate flatfish     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 39060..50430
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DTO64_RS00170 (DTO64_00170) - 39550..40017 (-) 468 WP_002359228.1 VOC family protein -
  DTO64_RS00175 (DTO64_00175) - 40082..41029 (+) 948 WP_002404711.1 YafY family protein -
  DTO64_RS00180 (DTO64_00180) - 41121..42374 (-) 1254 WP_002404712.1 glutamate-5-semialdehyde dehydrogenase -
  DTO64_RS00185 (DTO64_00185) proB 42361..43176 (-) 816 WP_002389550.1 glutamate 5-kinase -
  DTO64_RS00190 (DTO64_00190) - 43496..43981 (+) 486 WP_002359232.1 dUTP diphosphatase -
  DTO64_RS00195 (DTO64_00195) radA 44060..45430 (+) 1371 WP_114679326.1 DNA repair protein RadA Machinery gene
  DTO64_RS00200 (DTO64_00200) - 45534..46679 (+) 1146 WP_002368622.1 PIN/TRAM domain-containing protein -
  DTO64_RS00205 (DTO64_00205) ispF 46698..47171 (+) 474 WP_002359233.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -
  DTO64_RS00210 (DTO64_00210) gltX 47224..48681 (+) 1458 WP_002381335.1 glutamate--tRNA ligase -
  DTO64_RS00220 (DTO64_00220) epsC 48951..49490 (+) 540 WP_002356054.1 serine O-acetyltransferase EpsC -

Sequence

Protein


Download         Length: 456 a.a.        Molecular weight: 49787.30 Da        Isoelectric Point: 7.3489

>NTDB_id=302882 DTO64_RS00195 WP_114679326.1 44060..45430(+) (radA) [Enterococcus faecalis strain TY1 isolate flatfish]
MAKKAKVQFECQSCGYVSPKYLGRCPNCGQWNSMVEEVIQDTSDRRARVSLTGKKTQPQRLSEVVPKKEPRVKTELVELN
RVLGGGVVPGSLVLISGDPGIGKSTLLLQVSQQLAATGGTVLYVSGEESAEQIKLRAERLGTVNETFYLYAETDMHEISR
AIEKLEPDYVIIDSIQTMTQPDVTSVAGSVSQVRETTAELLKLAKTNGIAIFIVGHVTKEGSIAGPRMLEHMVDTVLYFE
GDKHHTFRILRAVKNRFGSTNEIGIFEMQTHGLVEVMNPSQVFLEERLEGATGSSIVVAMEGSRPILVEIQALVTPTMFG
NAKRTTTGLDFNRVSLIMAVLEKRAGLLLQNQDAYLKAAGGVKINEPAIDLALAISIASSYKEKGTSSSECFIGEIGLTG
EIRRVNSIEQRVREAQKLGFTKVYVPKNNLGGWEAPEGIEIIGVSTIGETLRKVFK

Nucleotide


Download         Length: 1371 bp        

>NTDB_id=302882 DTO64_RS00195 WP_114679326.1 44060..45430(+) (radA) [Enterococcus faecalis strain TY1 isolate flatfish]
ATGGCAAAAAAAGCAAAAGTTCAATTTGAATGTCAAAGCTGTGGGTATGTGTCTCCAAAATATCTCGGCCGCTGTCCTAA
TTGTGGACAATGGAATTCGATGGTGGAAGAGGTTATTCAAGATACTTCTGATCGTCGTGCTAGAGTAAGTTTGACTGGTA
AAAAGACACAACCACAACGGCTATCTGAAGTCGTACCTAAAAAAGAGCCGCGTGTAAAAACTGAATTAGTGGAGCTGAAT
CGGGTTTTAGGTGGTGGCGTTGTTCCAGGTTCGTTAGTTTTAATCAGTGGCGATCCCGGAATAGGTAAGTCAACATTGCT
CTTACAAGTATCGCAACAATTAGCAGCCACAGGTGGCACGGTTTTATATGTTTCTGGTGAAGAAAGTGCTGAGCAAATTA
AATTACGAGCGGAGCGTCTAGGCACCGTCAACGAGACATTTTACTTATACGCAGAAACAGATATGCACGAAATATCACGA
GCCATTGAAAAATTAGAACCAGACTATGTCATTATTGACTCGATTCAAACAATGACGCAGCCAGATGTTACCAGCGTTGC
TGGTAGTGTCAGTCAAGTCCGAGAAACAACTGCCGAATTATTGAAACTGGCAAAAACGAATGGGATTGCCATTTTTATTG
TCGGACACGTAACGAAGGAAGGGTCTATCGCAGGGCCACGGATGTTGGAACATATGGTAGACACGGTTCTTTATTTTGAA
GGAGATAAGCATCACACCTTTAGAATCTTACGGGCTGTCAAAAATCGGTTTGGCTCAACGAATGAGATTGGTATTTTTGA
AATGCAGACACATGGATTGGTTGAAGTTATGAATCCTTCTCAGGTCTTTTTAGAAGAACGTTTAGAAGGGGCCACCGGAT
CTTCGATTGTGGTAGCAATGGAAGGTTCACGCCCCATTTTGGTGGAAATCCAAGCGTTAGTTACCCCAACGATGTTTGGC
AATGCCAAGCGAACGACCACCGGGTTGGACTTTAATCGCGTTTCTTTAATCATGGCGGTTTTAGAAAAACGGGCAGGCCT
TTTATTACAAAACCAGGATGCGTATCTAAAAGCGGCTGGTGGAGTCAAAATTAATGAACCAGCTATTGATTTAGCGTTAG
CAATCAGTATTGCTTCTAGCTATAAAGAAAAAGGAACCTCATCTTCAGAATGTTTCATTGGTGAGATTGGTTTAACTGGT
GAAATCCGCCGCGTGAATAGTATTGAACAACGAGTGCGTGAAGCGCAAAAACTAGGCTTTACAAAAGTCTATGTCCCTAA
AAATAACCTTGGTGGCTGGGAAGCGCCTGAAGGGATTGAAATTATTGGCGTTTCAACAATTGGTGAAACGTTAAGAAAAG
TTTTTAAATAA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(65-221)

Subunit ChlI of Mg-chelatase (ChlI)

(362-429)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(9-36)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus mitis NCTC 12261

75.165

99.781

0.75
  radA Streptococcus pneumoniae Rx1

75.165

99.781

0.75
  radA Streptococcus pneumoniae D39

75.165

99.781

0.75
  radA Streptococcus pneumoniae R6

75.165

99.781

0.75
  radA Streptococcus pneumoniae TIGR4

75.165

99.781

0.75
  radA Streptococcus mitis SK321

75.165

99.781

0.75
  radA Bacillus subtilis subsp. subtilis str. 168

66.075

98.904

0.654

Multiple sequence alignment