Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   DT070_RS05795 Genome accession   NZ_CP031013
Coordinates   1202257..1203636 (-) Length   459 a.a.
NCBI ID   WP_122954535.1    Uniprot ID   -
Organism   Polaromonas sp. SP1     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1197257..1208636
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DT070_RS05765 (DT070_05765) - 1197874..1198479 (+) 606 WP_122954529.1 carboxymuconolactone decarboxylase family protein -
  DT070_RS05770 (DT070_05770) waaF 1198476..1199483 (-) 1008 WP_122954530.1 lipopolysaccharide heptosyltransferase II -
  DT070_RS05775 (DT070_05775) - 1199583..1199807 (-) 225 WP_122954531.1 zinc-finger domain-containing protein -
  DT070_RS05780 (DT070_05780) - 1199830..1200768 (-) 939 WP_122954532.1 branched-chain amino acid transaminase -
  DT070_RS05785 (DT070_05785) - 1200850..1201275 (-) 426 WP_122954533.1 glycerate kinase -
  DT070_RS05790 (DT070_05790) - 1201433..1202233 (-) 801 WP_122954534.1 metallophosphoesterase -
  DT070_RS05795 (DT070_05795) radA 1202257..1203636 (-) 1380 WP_122954535.1 DNA repair protein RadA Machinery gene
  DT070_RS05800 (DT070_05800) - 1203727..1204134 (+) 408 WP_122954536.1 5-carboxymethyl-2-hydroxymuconate Delta-isomerase -
  DT070_RS05805 (DT070_05805) - 1204228..1205214 (+) 987 WP_122954537.1 Bug family tripartite tricarboxylate transporter substrate binding protein -
  DT070_RS05810 (DT070_05810) - 1205310..1206284 (+) 975 WP_122954538.1 2-dehydropantoate 2-reductase -
  DT070_RS05815 (DT070_05815) - 1206335..1207228 (+) 894 WP_122954539.1 NAD(P)-dependent oxidoreductase -
  DT070_RS05820 (DT070_05820) - 1207250..1207717 (-) 468 WP_122954540.1 GNAT family N-acetyltransferase -
  DT070_RS05825 (DT070_05825) - 1207730..1208203 (-) 474 WP_122954541.1 RDD family protein -

Sequence

Protein


Download         Length: 459 a.a.        Molecular weight: 48415.70 Da        Isoelectric Point: 6.9932

>NTDB_id=302863 DT070_RS05795 WP_122954535.1 1202257..1203636(-) (radA) [Polaromonas sp. SP1]
MAKDKSIYTCTECGGTSPKWLGKCPHCNAWNTLIESAAESSSPVKNRYSSQFQSLAKASEVTTLSDIEAADMERTPTGHE
ELDRVLGGGIVEGGVVLIGGDPGIGKSTLLLQALDSLQRAGLNTLYVTGEESGAQVALRSRRLGLDGSQVKVLAEIQLEK
ILATLEATQPAIAVIDSIQTVYSDQLTSAPGSVAQVRECAAHLTRAAKSSGVCIVLVGHVTKEGALAGPRVLEHMVDTVL
YFEGDTHSSFRLVRAIKNRFGAVNEIGVFAMTEKGLKGVANPSAIFLSQHSEPVPGSCVLVTLEGTRPMLVEIQALVDNG
GPSPRRLSVGLDRDRLAMLLAVLHRHAGVACMDQDVFVNAVGGVRISEPAADLAVMLAITSSLRGKPLPKGFLAFGEVGL
AGEVRPAPRGQERLKEAAKLGFSVAVVPKANAPKKPIEGLTIHAVERVEEAMNVVRSLS

Nucleotide


Download         Length: 1380 bp        

>NTDB_id=302863 DT070_RS05795 WP_122954535.1 1202257..1203636(-) (radA) [Polaromonas sp. SP1]
ATGGCCAAAGACAAATCGATTTACACCTGCACCGAATGCGGCGGCACCAGCCCCAAGTGGCTGGGCAAATGCCCGCACTG
CAACGCCTGGAACACGCTGATCGAATCGGCCGCCGAGAGCAGCTCGCCCGTCAAGAACCGCTACAGCAGCCAGTTCCAGT
CGCTGGCCAAGGCGTCGGAGGTGACCACGCTGTCCGACATCGAAGCCGCCGACATGGAGCGCACGCCCACCGGGCATGAA
GAGCTGGACCGCGTGCTGGGCGGCGGCATCGTCGAAGGCGGCGTGGTGCTGATCGGCGGCGACCCGGGCATCGGCAAATC
GACGCTGCTGCTGCAGGCGCTGGATTCGCTGCAGCGCGCGGGGCTCAATACCCTTTACGTGACGGGTGAGGAAAGCGGCG
CGCAGGTGGCGCTGCGTTCGCGCCGGCTCGGGCTGGACGGCTCGCAGGTCAAGGTGCTGGCCGAAATCCAGCTCGAAAAA
ATCCTCGCCACGCTGGAGGCCACGCAGCCCGCGATTGCCGTCATCGATTCGATCCAGACGGTGTATTCCGACCAGCTCAC
CTCCGCGCCCGGCTCGGTCGCGCAGGTGCGTGAATGCGCGGCCCACCTCACGCGCGCGGCCAAGTCCAGCGGCGTGTGCA
TCGTGCTGGTGGGCCATGTGACCAAAGAGGGCGCGCTCGCCGGCCCGCGCGTGCTCGAGCACATGGTCGACACGGTGCTC
TACTTTGAAGGCGACACGCATTCGAGCTTTCGCCTGGTGCGCGCCATCAAGAACCGCTTCGGTGCGGTCAATGAAATCGG
CGTGTTCGCCATGACCGAAAAAGGCCTCAAGGGCGTGGCCAACCCGAGCGCGATCTTTTTGAGCCAGCACAGCGAGCCGG
TGCCCGGCAGCTGCGTGCTCGTTACGCTGGAGGGCACGCGCCCCATGCTGGTCGAAATCCAGGCGCTGGTCGACAACGGC
GGGCCCAGCCCGCGCCGCCTGTCGGTCGGCCTGGACCGCGACCGCCTGGCCATGCTGCTGGCCGTGCTGCACCGCCATGC
GGGCGTGGCCTGCATGGACCAGGACGTTTTTGTCAACGCCGTGGGCGGCGTGCGCATCAGCGAGCCCGCCGCCGACCTGG
CCGTGATGCTGGCCATCACCTCGTCCTTGCGCGGCAAGCCGCTGCCCAAAGGCTTTCTCGCGTTTGGCGAAGTCGGCCTG
GCCGGCGAAGTGCGGCCCGCCCCGCGCGGCCAGGAGCGCCTGAAGGAAGCCGCCAAACTGGGCTTTAGCGTGGCCGTGGT
GCCCAAGGCCAATGCGCCGAAGAAGCCGATCGAAGGCCTGACCATCCACGCGGTGGAACGGGTGGAGGAGGCGATGAACG
TGGTGCGCAGCCTGAGCTAG

Domains

Predicted by InterproScan.

Lon protease (S16) C-terminal proteolytic domain (Lon_C)

(362-453)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

KaiC (ATPase)

(76-256)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

49.234

99.564

0.49
  radA Streptococcus mitis NCTC 12261

45.475

98.693

0.449
  radA Streptococcus pneumoniae D39

47.209

93.682

0.442
  radA Streptococcus pneumoniae TIGR4

47.209

93.682

0.442
  radA Streptococcus mitis SK321

47.209

93.682

0.442
  radA Streptococcus pneumoniae R6

47.209

93.682

0.442
  radA Streptococcus pneumoniae Rx1

47.209

93.682

0.442

Multiple sequence alignment