Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   DT536_RS07600 Genome accession   NZ_CP031011
Coordinates   1560463..1561263 (+) Length   266 a.a.
NCBI ID   WP_058870302.1    Uniprot ID   -
Organism   Acinetobacter johnsonii strain LXL_C1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1555463..1566263
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DT536_RS07570 (DT536_07570) - 1555675..1556091 (+) 417 WP_004983505.1 hypothetical protein -
  DT536_RS07575 (DT536_07575) - 1556232..1557512 (+) 1281 WP_058870304.1 glycosyltransferase family 4 protein -
  DT536_RS07580 (DT536_07580) - 1557705..1558256 (+) 552 WP_058870303.1 phosphatase PAP2 family protein -
  DT536_RS07585 (DT536_07585) iscX 1558346..1558546 (+) 201 WP_004697668.1 Fe-S cluster assembly protein IscX -
  DT536_RS07590 (DT536_07590) ndk 1558657..1559088 (+) 432 WP_004983508.1 nucleoside-diphosphate kinase -
  DT536_RS07595 (DT536_07595) rlmN 1559211..1560446 (+) 1236 WP_004697671.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  DT536_RS07600 (DT536_07600) pilF 1560463..1561263 (+) 801 WP_058870302.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  DT536_RS07605 (DT536_07605) - 1561254..1562030 (+) 777 WP_058870301.1 helix-turn-helix domain-containing protein -
  DT536_RS07610 (DT536_07610) ispG 1562049..1563164 (+) 1116 WP_004697677.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  DT536_RS07615 (DT536_07615) hisS 1563161..1564456 (+) 1296 WP_058870300.1 histidine--tRNA ligase -
  DT536_RS07620 (DT536_07620) - 1564484..1565182 (+) 699 WP_058870299.1 YfgM family protein -

Sequence

Protein


Download         Length: 266 a.a.        Molecular weight: 30044.07 Da        Isoelectric Point: 9.1790

>NTDB_id=302847 DT536_RS07600 WP_058870302.1 1560463..1561263(+) (pilF) [Acinetobacter johnsonii strain LXL_C1]
MKKPVSKLCLALTLGVSVWYLVGCQTVNTKGDPSKAVQVRTQLAAEYIKSNDYDAAKRTLDQALDIDSRDASANMMMGVL
LQREGSPQNVEKAERYFKHAIAAEPKNAQARNNYGTYLYQIGRYNDAIDQLRVAGSTLGYDQRYRALENLGRAYLQVGRV
AEAEAAFKQALQVNSGAYLAMIEMAEISYLRQQNAEATQYYEQFVRAVGEKNLDARALWIGIRIARANHDTMGSQVLVNQ
LRALYPDSQEYKRYLQLQYTTEAVWK

Nucleotide


Download         Length: 801 bp        

>NTDB_id=302847 DT536_RS07600 WP_058870302.1 1560463..1561263(+) (pilF) [Acinetobacter johnsonii strain LXL_C1]
TTGAAAAAACCCGTTTCTAAACTTTGCCTTGCGCTGACACTGGGTGTGTCAGTGTGGTACTTGGTGGGTTGCCAAACTGT
GAATACGAAAGGTGATCCAAGTAAAGCAGTACAAGTTAGAACGCAGTTGGCGGCTGAATATATTAAGTCGAATGATTATG
ATGCTGCGAAACGTACATTGGATCAGGCATTAGACATCGACTCTCGTGATGCATCTGCAAATATGATGATGGGCGTGTTG
TTACAGCGTGAAGGCAGTCCACAAAATGTGGAAAAGGCAGAGCGTTATTTTAAGCATGCAATTGCGGCTGAGCCAAAAAA
TGCACAGGCTCGTAACAACTACGGTACGTATTTATATCAAATTGGGCGTTATAATGACGCGATTGATCAACTTCGAGTTG
CAGGTTCAACTTTAGGATATGATCAACGTTATCGAGCATTGGAAAATTTAGGTCGTGCGTATCTACAAGTAGGTCGAGTG
GCGGAAGCAGAAGCAGCTTTTAAGCAAGCCCTACAGGTAAACTCAGGTGCCTATCTTGCCATGATCGAAATGGCGGAAAT
TTCATATTTACGTCAGCAAAATGCTGAAGCTACGCAGTACTATGAACAGTTTGTGCGAGCGGTTGGCGAGAAAAACTTAG
ATGCACGTGCGCTTTGGATTGGGATCCGAATTGCGCGAGCAAATCATGACACGATGGGTTCGCAAGTACTGGTCAACCAG
CTACGTGCATTGTATCCAGACAGTCAAGAATACAAACGTTATTTGCAATTACAGTACACTACTGAGGCCGTATGGAAGTA
A

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_19)

(89-147)

Tetratricopeptide repeat (TPR_8)

(39-68)

Tetratricopeptide repeat (TPR_16)

(149-206)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

69.03

100

0.695
  pilF Acinetobacter baylyi ADP1

64.662

100

0.647

Multiple sequence alignment