Detailed information    

insolico Bioinformatically predicted

Overview

Name   rapF   Type   Regulator
Locus tag   DS740_RS20240 Genome accession   NZ_CP030937
Coordinates   3873858..3875003 (+) Length   381 a.a.
NCBI ID   WP_015250985.1    Uniprot ID   -
Organism   Bacillus sp. DM2     
Function   inhibit the DNA-binding function of ComA (predicted from homology)   
Competence regulation

Genomic Context

Location: 3868858..3880003
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DS740_RS20215 (DS740_20215) - 3868874..3870154 (+) 1281 WP_003227558.1 pitrilysin family protein -
  DS740_RS20220 (DS740_20220) albG 3870151..3870852 (+) 702 WP_043940223.1 antilisterial bacteriocin subtilosin biosynthesis protein AlbG -
  DS740_RS20225 (DS740_20225) - 3870857..3872233 (-) 1377 WP_043940224.1 YncE family protein -
  DS740_RS20230 (DS740_20230) - 3872273..3873628 (-) 1356 WP_043940225.1 YncE family protein -
  DS740_RS20240 (DS740_20240) rapF 3873858..3875003 (+) 1146 WP_015250985.1 response regulator aspartate phosphatase RapF Regulator
  DS740_RS20245 (DS740_20245) phrF 3874987..3875106 (+) 120 WP_009968329.1 phosphatase RapF inhibitor PhrF Regulator
  DS740_RS20250 (DS740_20250) - 3875359..3875838 (+) 480 WP_015714892.1 hypothetical protein -
  DS740_RS20255 (DS740_20255) - 3875987..3876775 (+) 789 WP_015714893.1 membrane protein -
  DS740_RS20260 (DS740_20260) - 3876762..3877436 (+) 675 WP_015714894.1 hypothetical protein -
  DS740_RS20265 (DS740_20265) - 3877446..3878243 (+) 798 WP_231573542.1 hypothetical protein -
  DS740_RS20270 (DS740_20270) - 3878246..3878893 (+) 648 WP_015714896.1 ABC transporter ATP-binding protein -
  DS740_RS20275 (DS740_20275) - 3878886..3879446 (+) 561 WP_015714897.1 hypothetical protein -

Sequence

Protein


Download         Length: 381 a.a.        Molecular weight: 45590.06 Da        Isoelectric Point: 5.0624

>NTDB_id=302639 DS740_RS20240 WP_015250985.1 3873858..3875003(+) (rapF) [Bacillus sp. DM2]
MTGVISSSSIGEKINEWYMYIRRFSIPDAEYLRREIKQELDQMEEDQDLHLYYSLMEFRHNLMLEYLEPLEKMRIEEQPR
LSDLLLEIDKKQARLTGLLEYYFNFFRGMYELDQREYLSAIKFFKKAESKLIFVKDRIEKAEFFFKMSESYYYMKQTYFS
MDYARQAYEIYKENEAYNIRLLQCHSLFATNFLDLKQYEDAVSHFQKAYSMAEAEKQPQLMGRTLYNIGLCKNSQSQYED
AIPYFKRAIAVFEESNILPSLPQAYFLITQIHYKLGKMDKAHEYHSKGMAYSQKAGDVIYLSEFEFLKSLYLSGPDEEAI
QGFFDFLESKMLYADLEDFAIDVAKYYHERKNFQKASAYFLKVEQVRQLIQGGVSLYEIEV

Nucleotide


Download         Length: 1146 bp        

>NTDB_id=302639 DS740_RS20240 WP_015250985.1 3873858..3875003(+) (rapF) [Bacillus sp. DM2]
GTGACAGGTGTCATATCTTCTTCTTCCATCGGAGAAAAGATTAACGAATGGTATATGTACATACGCCGATTCAGCATACC
CGATGCAGAATATTTGCGACGAGAAATCAAGCAAGAGCTGGATCAAATGGAAGAAGATCAAGACCTTCATTTGTACTATT
CACTGATGGAGTTTCGGCACAACCTGATGCTTGAGTACCTTGAACCGTTAGAAAAAATGAGGATTGAGGAACAGCCGAGA
CTGTCTGATCTGCTGCTTGAGATTGATAAAAAACAGGCTCGTTTAACTGGTCTGCTTGAGTACTATTTTAATTTCTTCAG
AGGCATGTATGAGCTGGATCAGCGGGAATATCTGTCGGCTATTAAATTTTTCAAAAAGGCCGAAAGCAAGCTGATATTCG
TTAAGGATCGGATAGAGAAAGCTGAGTTTTTCTTTAAGATGTCTGAATCTTATTACTATATGAAACAAACGTATTTTTCA
ATGGACTATGCACGGCAAGCATATGAAATATACAAAGAAAATGAAGCTTATAATATAAGATTGCTGCAGTGTCATTCTTT
ATTTGCCACCAATTTTTTAGATTTAAAACAGTATGAGGATGCCGTCTCACATTTTCAAAAAGCTTATTCTATGGCAGAAG
CTGAAAAGCAGCCCCAATTAATGGGAAGAACTTTGTACAATATCGGGCTTTGTAAAAACAGCCAAAGCCAATATGAGGAT
GCCATACCTTATTTCAAAAGAGCAATAGCTGTTTTTGAAGAATCAAATATTCTTCCTTCCTTACCTCAAGCGTATTTTTT
AATTACACAGATCCATTATAAATTAGGAAAAATGGATAAAGCTCATGAATATCATAGTAAGGGAATGGCTTATTCACAAA
AGGCCGGAGATGTAATATATTTATCAGAGTTTGAATTTTTGAAATCTTTATACTTATCAGGCCCGGATGAAGAAGCAATT
CAAGGATTTTTTGATTTTCTCGAAAGTAAAATGTTGTATGCTGATCTTGAAGATTTCGCTATTGATGTGGCAAAATATTA
TCATGAACGTAAAAATTTTCAAAAAGCTTCTGCTTATTTTTTGAAGGTGGAACAAGTAAGGCAACTTATTCAAGGAGGAG
TGAGTTTGTATGAAATTGAAGTCTAA

Domains

Predicted by InterproScan.

response regulator aspartate phosphatase H, N terminal (RapH_N)

(4-65)

Tetratricopeptide repeat (TPR_12)

(223-290)

Anaphase-promoting complex, cyclosome, subunit 3 (ANAPC3)

(132-207)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rapF Bacillus subtilis subsp. subtilis str. 168

99.213

100

0.992
  rapC Bacillus subtilis subsp. subtilis str. 168

57.368

99.738

0.572

Multiple sequence alignment