Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   TRQ2_RS01860 Genome accession   NC_010483
Coordinates   353349..354233 (+) Length   294 a.a.
NCBI ID   WP_004081293.1    Uniprot ID   A0A0F6AJU4
Organism   Thermotoga sp. RQ2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 348349..359233
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  TRQ2_RS01845 (TRQ2_0363) lnt 349152..350615 (-) 1464 WP_012310499.1 apolipoprotein N-acyltransferase -
  TRQ2_RS01850 (TRQ2_0364) - 350608..351759 (-) 1152 WP_012310500.1 DegT/DnrJ/EryC1/StrS aminotransferase family protein -
  TRQ2_RS01855 (TRQ2_0365) - 351856..353235 (-) 1380 WP_012310501.1 DegQ family serine endoprotease -
  TRQ2_RS01860 (TRQ2_0366) pilA 353349..354233 (+) 885 WP_004081293.1 signal recognition particle-docking protein FtsY Machinery gene
  TRQ2_RS01865 (TRQ2_0367) - 354230..354886 (+) 657 WP_004081295.1 DUF2225 domain-containing protein -
  TRQ2_RS01870 (TRQ2_0368) - 354862..356526 (+) 1665 WP_004081297.1 peptidoglycan DD-metalloendopeptidase family protein -
  TRQ2_RS01875 (TRQ2_0369) - 356528..356941 (+) 414 WP_004081299.1 RrF2 family transcriptional regulator -
  TRQ2_RS01880 (TRQ2_0370) - 356960..357154 (-) 195 WP_004081301.1 hypothetical protein -
  TRQ2_RS01885 (TRQ2_0371) sfsA 357158..357826 (-) 669 WP_012310502.1 DNA/RNA nuclease SfsA -
  TRQ2_RS01890 (TRQ2_0372) - 357823..358302 (-) 480 WP_011943018.1 flavin reductase family protein -

Sequence

Protein


Download         Length: 294 a.a.        Molecular weight: 32046.26 Da        Isoelectric Point: 5.3215

>NTDB_id=30261 TRQ2_RS01860 WP_004081293.1 353349..354233(+) (pilA) [Thermotoga sp. RQ2]
MGLFDFLKKGLQKTKETFFGRVVKLLKGKKLDDETREELEELLIQADVGVETTEYILERLEEKDGDALESLKEIILEILN
FDTKLNVPPEPPFVIMVVGVNGTGKTTSCGKLAKMFVDEGKSVVLAAADTFRAAAIEQLKIWGERVGATVISHSEGADPA
AVAFDAVAHALARNKDVVIIDTAGRLHTKKNLMEELRKVHRVVKKKIPDAPHETLLVIDATTGQNGLVQAKIFKEAVNVT
GIILTKLDGTAKGGITLAIARELGIPIKFIGVGEKAEDLRPFDPEAFVEVLLSE

Nucleotide


Download         Length: 885 bp        

>NTDB_id=30261 TRQ2_RS01860 WP_004081293.1 353349..354233(+) (pilA) [Thermotoga sp. RQ2]
ATGGGACTCTTCGATTTTTTGAAAAAGGGACTCCAGAAGACGAAAGAAACGTTTTTTGGACGAGTGGTGAAGCTCCTCAA
AGGAAAAAAGCTGGACGATGAAACCCGCGAGGAGCTGGAAGAACTTCTCATTCAAGCGGACGTGGGAGTCGAGACAACGG
AATACATTCTGGAGAGATTGGAAGAGAAGGACGGAGACGCTCTCGAGTCTCTCAAGGAAATCATTCTGGAAATTCTGAAC
TTCGATACGAAGCTGAACGTTCCTCCTGAACCCCCGTTCGTGATCATGGTTGTTGGTGTCAACGGAACGGGAAAGACCAC
CTCCTGTGGAAAGCTCGCAAAGATGTTCGTGGATGAAGGAAAGAGTGTGGTGCTCGCTGCGGCGGATACTTTCAGAGCAG
CCGCCATAGAACAGCTGAAGATATGGGGAGAACGTGTCGGTGCCACGGTGATATCCCACTCGGAAGGAGCAGATCCGGCC
GCCGTTGCCTTTGACGCGGTGGCTCACGCTCTCGCCAGAAACAAAGATGTGGTCATAATAGACACCGCCGGAAGACTTCA
CACGAAGAAGAATCTCATGGAGGAATTGCGGAAGGTTCACAGAGTTGTCAAGAAGAAGATCCCCGACGCTCCGCACGAAA
CGCTCCTCGTGATAGACGCCACCACCGGCCAAAACGGACTTGTTCAGGCAAAAATATTCAAAGAAGCAGTGAATGTCACC
GGTATAATTCTCACAAAGCTCGACGGAACCGCGAAAGGCGGAATAACTCTGGCCATTGCCCGGGAACTGGGAATTCCCAT
AAAGTTCATCGGTGTCGGAGAAAAGGCAGAAGACCTGAGGCCTTTCGATCCTGAGGCCTTTGTGGAGGTATTGCTTTCAG
AATGA

Domains

Predicted by InterproScan.

SRP54-type protein, helical bundle domain (SRP54_N)

(22-65)

SRP54-type protein, GTPase domain (SRP54)

(92-292)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0F6AJU4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Neisseria gonorrhoeae MS11

50.331

100

0.517

Multiple sequence alignment