Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   DS740_RS01955 Genome accession   NZ_CP030937
Coordinates   363718..364167 (-) Length   149 a.a.
NCBI ID   WP_014475770.1    Uniprot ID   A0A0D1KPR3
Organism   Bacillus sp. DM2     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 358718..369167
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DS740_RS01920 (DS740_01920) - 358869..359549 (-) 681 WP_003234624.1 amino acid ABC transporter permease -
  DS740_RS01925 (DS740_01925) - 359559..360422 (-) 864 WP_080121173.1 transporter substrate-binding domain-containing protein -
  DS740_RS22660 - 360583..360780 (+) 198 WP_120363256.1 hypothetical protein -
  DS740_RS01935 (DS740_01935) - 360800..361255 (+) 456 WP_024572178.1 RDD family protein -
  DS740_RS01940 (DS740_01940) - 361337..361669 (+) 333 WP_003246247.1 YckD family protein -
  DS740_RS01945 (DS740_01945) bglC 361823..363256 (+) 1434 WP_038828490.1 6-phospho-beta-glucosidase -
  DS740_RS01950 (DS740_01950) nin/comJ 363293..363691 (-) 399 WP_021481824.1 DNA-entry nuclease inhibitor Regulator
  DS740_RS01955 (DS740_01955) nucA/comI 363718..364167 (-) 450 WP_014475770.1 DNA-entry nuclease Machinery gene
  DS740_RS01960 (DS740_01960) tlpC 364335..366056 (-) 1722 WP_021481821.1 methyl-accepting chemotaxis protein TlpC -
  DS740_RS01965 (DS740_01965) hxlB 366166..366723 (-) 558 WP_041850679.1 6-phospho-3-hexuloisomerase -
  DS740_RS01970 (DS740_01970) hxlA 366729..367361 (-) 633 WP_041850680.1 3-hexulose-6-phosphate synthase -
  DS740_RS01975 (DS740_01975) hxlR 367594..367956 (+) 363 WP_003246265.1 transcriptional activator HxlR -

Sequence

Protein


Download         Length: 149 a.a.        Molecular weight: 16440.39 Da        Isoelectric Point: 4.5282

>NTDB_id=302571 DS740_RS01955 WP_014475770.1 363718..364167(-) (nucA/comI) [Bacillus sp. DM2]
MNITTDIIKTILLVIVIIAAAAVGLIKGDFFSADQKTSQTKEYDETIAFPSDRYPETAKHIKDAINEGHSEVCTIDRDGA
EERREQSLKDVPSKKGYDRDEWPMAMCKEGGEGASVEYISPADNRGAGSWVGHQLTDYPDGTKVLFTIQ

Nucleotide


Download         Length: 450 bp        

>NTDB_id=302571 DS740_RS01955 WP_014475770.1 363718..364167(-) (nucA/comI) [Bacillus sp. DM2]
GTGAACATCACGACGGACATCATAAAAACAATACTTCTCGTCATCGTCATCATAGCAGCTGCAGCTGTCGGCCTGATAAA
AGGAGACTTTTTCTCAGCTGATCAAAAAACGTCTCAAACGAAAGAATATGATGAAACAATCGCCTTCCCTTCTGACCGTT
ATCCCGAAACGGCCAAGCATATTAAGGATGCGATAAATGAGGGGCACTCAGAGGTGTGCACTATCGACAGAGACGGAGCT
GAGGAACGCCGCGAGCAATCATTAAAGGACGTACCTTCCAAAAAGGGGTATGACAGAGATGAATGGCCAATGGCCATGTG
CAAAGAAGGCGGTGAGGGAGCTTCTGTAGAATACATTTCTCCCGCTGACAACCGCGGAGCAGGCTCTTGGGTCGGGCATC
AGCTTACCGATTACCCAGACGGCACAAAGGTTTTATTCACAATTCAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(52-148)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0D1KPR3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

98.658

100

0.987

Multiple sequence alignment