Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   DTQ05_RS00540 Genome accession   NZ_CP030931
Coordinates   96757..98133 (+) Length   458 a.a.
NCBI ID   WP_029343076.1    Uniprot ID   -
Organism   Exiguobacterium acetylicum strain AMCC 101217     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 91757..103133
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DTQ05_RS00520 - 92258..92713 (+) 456 WP_023466578.1 CtsR family transcriptional regulator -
  DTQ05_RS00525 - 92739..93167 (+) 429 WP_029343073.1 hypothetical protein -
  DTQ05_RS00530 - 93160..94233 (+) 1074 WP_149426603.1 protein arginine kinase -
  DTQ05_RS00535 clpC 94233..96680 (+) 2448 WP_023466588.1 ATP-dependent protease ATP-binding subunit ClpC -
  DTQ05_RS00540 radA 96757..98133 (+) 1377 WP_029343076.1 DNA repair protein RadA Machinery gene
  DTQ05_RS00545 - 98261..99346 (+) 1086 WP_023466590.1 PIN/TRAM domain-containing protein -
  DTQ05_RS00550 ispD 99479..100162 (+) 684 WP_149426604.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  DTQ05_RS00555 ispF 100165..100647 (+) 483 WP_029343079.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -
  DTQ05_RS00560 gltX 100691..102139 (+) 1449 WP_023466595.1 glutamate--tRNA ligase -
  DTQ05_RS00565 epsC 102367..103086 (+) 720 WP_255343794.1 serine O-acetyltransferase EpsC -

Sequence

Protein


Download         Length: 458 a.a.        Molecular weight: 50039.64 Da        Isoelectric Point: 6.6525

>NTDB_id=302477 DTQ05_RS00540 WP_029343076.1 96757..98133(+) (radA) [Exiguobacterium acetylicum strain AMCC 101217]
MAKLKTKFVCQSCGTESPKWMGRCSGCGEWNTMVEEVVEEKKGRRGAAFVHTTTKQLKPERLANIVSQEESRVFTGSGEF
DRVLGGGIVPGSMVLVGGDPGIGKSTILLQTSARLAQRGEKVLYISGEESLKQTKLRAERLGLPTQDLFVLSETDMNMIE
RVVDEEQPRFLIIDSIQTVYIDEIQSAPGSVTQVRECTAMLMKIAKSRGIAIFIVGHVTKQGSIAGPRLLEHMVDAVLYF
EGERHHTFRILRAVKNRFGSTNEIGIFEMRESGLEEVLNPSEIFLEERTSGVSGSTIVASMEGTRTVLVELQALISPTSF
GNPRRMATGIDQNKVALLMAVLEKRSGLLLQTQDAYLKAAGGVKLDEPAIDLAVCVAIASSFRDKPTRPTDVVIGEVGLT
GEVRRVSRIEQRVAEAAKLGFTRAIIPKNNLGGWTYPDGITVVGVESVDEALRETIPF

Nucleotide


Download         Length: 1377 bp        

>NTDB_id=302477 DTQ05_RS00540 WP_029343076.1 96757..98133(+) (radA) [Exiguobacterium acetylicum strain AMCC 101217]
TTGGCTAAGTTAAAAACAAAATTCGTTTGTCAAAGCTGTGGGACCGAATCACCGAAATGGATGGGACGTTGTTCCGGGTG
CGGAGAATGGAACACGATGGTCGAAGAAGTCGTCGAAGAGAAAAAAGGGCGGCGCGGTGCAGCTTTCGTTCATACGACAA
CAAAACAATTGAAGCCAGAGCGCCTCGCGAACATCGTCTCGCAAGAAGAGAGTCGCGTCTTTACCGGAAGTGGTGAATTC
GACCGTGTCCTCGGAGGCGGTATCGTTCCAGGTTCGATGGTGCTTGTTGGAGGAGATCCGGGAATCGGGAAGTCGACGAT
CTTACTTCAGACGAGCGCACGTCTTGCCCAACGCGGCGAAAAAGTGCTCTACATCTCAGGAGAAGAATCGCTCAAACAAA
CTAAATTGCGCGCGGAACGGCTTGGCTTACCGACTCAGGATTTGTTCGTCCTTAGTGAGACGGATATGAATATGATTGAA
CGTGTCGTCGATGAAGAACAGCCACGTTTTTTAATCATTGACTCGATTCAAACCGTCTACATCGATGAAATCCAGTCTGC
ACCCGGGAGTGTGACACAGGTACGTGAATGTACGGCGATGCTGATGAAAATTGCGAAGAGTCGCGGGATTGCGATCTTCA
TCGTCGGTCACGTCACGAAACAGGGTTCGATTGCTGGACCACGTTTACTGGAGCACATGGTGGATGCTGTCTTGTATTTC
GAAGGCGAACGTCATCATACGTTCCGGATTCTGCGTGCCGTCAAAAACCGATTTGGTTCAACGAATGAGATCGGAATTTT
TGAGATGCGGGAGTCTGGTCTTGAAGAGGTCTTGAATCCATCAGAAATTTTCCTCGAGGAGCGGACGTCCGGTGTATCCG
GTTCGACGATCGTCGCTTCCATGGAGGGGACACGGACGGTCCTTGTCGAACTGCAAGCACTGATTTCACCGACGTCATTC
GGAAATCCAAGACGGATGGCGACCGGAATCGATCAAAACAAGGTAGCCTTATTGATGGCTGTGCTTGAGAAACGGTCGGG
TCTTCTCCTTCAGACACAAGATGCGTACCTCAAGGCAGCCGGTGGTGTGAAGCTTGATGAACCGGCGATTGACTTGGCGG
TTTGTGTGGCAATTGCTTCTAGTTTCCGTGACAAGCCGACGCGTCCGACAGATGTCGTGATTGGTGAGGTCGGATTGACA
GGTGAGGTTCGCCGTGTATCGCGGATTGAACAACGTGTAGCAGAAGCAGCTAAACTCGGTTTCACGCGGGCGATCATTCC
GAAAAACAACTTGGGTGGTTGGACGTATCCGGACGGAATTACGGTCGTCGGTGTCGAGAGTGTGGACGAAGCATTACGTG
AAACGATTCCGTTTTAA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(83-222)

Subunit ChlI of Mg-chelatase (ChlI)

(364-430)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

72.588

99.563

0.723
  radA Streptococcus pneumoniae Rx1

62.775

99.127

0.622
  radA Streptococcus pneumoniae D39

62.775

99.127

0.622
  radA Streptococcus pneumoniae R6

62.775

99.127

0.622
  radA Streptococcus pneumoniae TIGR4

62.775

99.127

0.622
  radA Streptococcus mitis NCTC 12261

62.775

99.127

0.622
  radA Streptococcus mitis SK321

62.775

99.127

0.622

Multiple sequence alignment