Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   AVIN_RS07435 Genome accession   NC_012560
Coordinates   1595326..1597035 (+) Length   569 a.a.
NCBI ID   WP_012700247.1    Uniprot ID   C1DRU4
Organism   Azotobacter vinelandii DJ     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1590326..1602035
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AVIN_RS07405 (Avin_16110) - 1590724..1591758 (-) 1035 WP_012700241.1 general secretion pathway protein GspK -
  AVIN_RS07410 (Avin_16120) - 1591758..1592369 (-) 612 WP_012700242.1 type II secretion system protein J -
  AVIN_RS07415 (Avin_16130) - 1592543..1592989 (-) 447 WP_012700243.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  AVIN_RS07420 (Avin_16140) - 1592991..1593362 (-) 372 WP_012700244.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  AVIN_RS07425 (Avin_16150) gspG 1593363..1593800 (-) 438 WP_012700245.1 type II secretion system major pseudopilin GspG -
  AVIN_RS07430 (Avin_16160) - 1593822..1595021 (-) 1200 WP_012700246.1 type II secretion system F family protein -
  AVIN_RS07435 (Avin_16170) pilF 1595326..1597035 (+) 1710 WP_012700247.1 GspE/PulE family protein Machinery gene
  AVIN_RS07440 (Avin_16180) cysC 1597051..1597644 (+) 594 WP_012700248.1 adenylyl-sulfate kinase -
  AVIN_RS07445 (Avin_16190) - 1597641..1599053 (+) 1413 WP_012700249.1 FAD/NAD(P)-binding protein -
  AVIN_RS07450 (Avin_16210) - 1599061..1600335 (+) 1275 WP_231876247.1 hypothetical protein -
  AVIN_RS07455 (Avin_16220) - 1600320..1601426 (+) 1107 WP_012700252.1 DUF1972 domain-containing protein -

Sequence

Protein


Download         Length: 569 a.a.        Molecular weight: 62316.84 Da        Isoelectric Point: 6.3802

>NTDB_id=30237 AVIN_RS07435 WP_012700247.1 1595326..1597035(+) (pilF) [Azotobacter vinelandii DJ]
MGVPWIGAFPLVFRGVAMRLGERLVRAGLVTEDDVQRALGLQQQAGGRLGSIFIRMGALSEDALLVVLHEQLGLPVLDGA
GMPVPSTAYAFMSGLPLRLGWLLDNNVVLWEEAGRVHVLAKDPNDSRIGDLLGYTFGDRELVRYLARAQDVDTLVDQVRR
ESAVSDLFRDDRQTLKSLIEEAPVVELVNNLLAQAVDSGASDIHVEPEETRFTVRMRVDGVLHTRMVQPYERYPAIGSRI
KLIAGLDIAEKRLPQDGRITLRLSGKDMDIRVSTAPGVFGESIVMRLLPKNRGALSLGSLGFESDHIELLRQWLAFPNGI
VLVTGPTGSGKSTTLYSALEEMRDGSNKIITVEDPVEYQVPGVTQIQAHAEIGYTFARALRAILRQDPDTIMIGEIRDLD
TAQIAIQSALTGHLVLSTLHTNDAVSAFTRLIDMGLEPFLVAASVRGVQAQRLVRKLCGHCAVRDEAPALPAAWQAMGRR
VAEGDWKRACGCAHCHQTGYRGRMGIYELVPLSASLQQLVNRQAPLQEMKALIKGQGHRGLLEDGLIKASKGLTSIEEVM
RVACVEQEF

Nucleotide


Download         Length: 1710 bp        

>NTDB_id=30237 AVIN_RS07435 WP_012700247.1 1595326..1597035(+) (pilF) [Azotobacter vinelandii DJ]
ATGGGCGTCCCGTGGATTGGGGCTTTCCCGCTGGTATTCCGAGGGGTGGCGATGAGATTGGGTGAACGGCTGGTCCGGGC
CGGGCTGGTCACTGAGGATGATGTGCAGCGCGCCTTGGGGTTGCAGCAGCAGGCGGGTGGCCGGCTCGGCTCCATTTTCA
TTCGCATGGGCGCGCTTTCCGAGGATGCCTTGCTGGTTGTGCTGCATGAGCAGTTGGGGTTGCCGGTTCTGGACGGGGCC
GGGATGCCGGTCCCGTCCACTGCCTACGCCTTCATGTCCGGTCTTCCGTTACGGCTGGGCTGGTTGCTCGACAACAATGT
AGTGCTGTGGGAAGAGGCCGGCAGGGTGCACGTATTGGCCAAGGACCCGAACGATTCGCGGATCGGGGATCTGCTGGGCT
ACACCTTCGGTGACCGGGAACTGGTGCGCTATCTGGCCCGCGCTCAGGATGTCGACACGCTCGTCGATCAGGTGCGCAGG
GAGAGCGCCGTATCCGATCTGTTTCGCGATGATCGCCAGACCCTCAAGAGCCTGATCGAAGAAGCGCCGGTCGTCGAGCT
GGTGAACAATCTCCTGGCGCAGGCGGTCGATTCGGGAGCTTCCGACATTCATGTCGAGCCCGAGGAGACCCGTTTTACCG
TGCGGATGCGAGTCGACGGCGTTCTGCACACGCGCATGGTGCAGCCTTACGAGCGTTATCCGGCGATCGGTTCGCGCATC
AAGCTGATCGCCGGGCTGGATATCGCCGAGAAGCGTTTGCCGCAGGATGGCCGGATTACCTTGCGCCTGAGCGGAAAGGA
CATGGATATCCGGGTTTCCACGGCGCCGGGAGTTTTCGGCGAGTCGATCGTCATGCGGTTGTTGCCGAAGAACCGTGGTG
CCCTGTCGCTGGGCAGCCTGGGTTTCGAGTCCGACCATATCGAGTTGCTCAGGCAGTGGCTGGCATTTCCTAATGGCATT
GTGCTAGTAACTGGGCCCACGGGGTCCGGCAAGTCCACGACCCTGTATTCGGCGCTCGAGGAGATGCGCGATGGCAGCAA
CAAGATCATCACCGTGGAGGACCCCGTCGAGTATCAGGTACCGGGCGTGACGCAGATCCAGGCGCACGCGGAAATCGGCT
ACACCTTCGCTCGTGCGCTGCGGGCCATTCTGCGCCAGGACCCGGACACCATCATGATCGGTGAGATTCGCGACCTGGAT
ACCGCGCAGATCGCCATCCAGTCCGCTTTGACAGGGCACCTGGTGTTGTCGACGCTGCATACCAATGACGCGGTTTCGGC
CTTCACCCGCCTGATCGACATGGGGTTGGAGCCTTTTCTGGTGGCCGCTTCGGTGCGCGGTGTTCAGGCGCAGCGTCTGG
TGCGCAAGCTCTGCGGGCATTGCGCGGTGCGCGACGAGGCGCCGGCCTTGCCGGCTGCCTGGCAGGCAATGGGACGTCGC
GTCGCGGAGGGCGACTGGAAACGGGCATGCGGTTGTGCCCATTGCCACCAGACCGGCTATCGCGGTCGCATGGGGATATA
CGAGTTGGTACCCCTGAGCGCTTCCCTGCAGCAGTTGGTCAATCGCCAGGCGCCTCTGCAGGAGATGAAGGCGCTGATCA
AGGGTCAGGGGCACCGGGGCCTGCTGGAGGATGGATTGATCAAGGCCAGCAAGGGTCTGACCAGTATCGAGGAGGTCATG
CGTGTTGCTTGTGTCGAACAGGAATTCTGA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(190-456)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(54-161)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB C1DRU4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Thermus thermophilus HB27

38.38

99.824

0.383
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.889

98.067

0.381

Multiple sequence alignment