Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   DTO10_RS12220 Genome accession   NZ_CP030926
Coordinates   2543980..2545368 (+) Length   462 a.a.
NCBI ID   WP_098174999.1    Uniprot ID   A0AAX0S9K5
Organism   Peribacillus butanolivorans strain PHB-7a     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2538980..2550368
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DTO10_RS12200 (DTO10_12200) - 2539080..2539541 (+) 462 WP_053349092.1 CtsR family transcriptional regulator -
  DTO10_RS12205 (DTO10_12205) - 2539562..2540113 (+) 552 WP_098175000.1 UvrB/UvrC motif-containing protein -
  DTO10_RS12210 (DTO10_12210) - 2540110..2541186 (+) 1077 WP_053349090.1 protein arginine kinase -
  DTO10_RS12215 (DTO10_12215) clpC 2541305..2543746 (+) 2442 WP_053349089.1 ATP-dependent protease ATP-binding subunit ClpC -
  DTO10_RS12220 (DTO10_12220) radA 2543980..2545368 (+) 1389 WP_098174999.1 DNA repair protein RadA Machinery gene
  DTO10_RS12225 (DTO10_12225) disA 2545371..2546444 (+) 1074 WP_053349087.1 DNA integrity scanning diadenylate cyclase DisA -
  DTO10_RS12230 (DTO10_12230) - 2546671..2547768 (+) 1098 WP_116821267.1 PIN/TRAM domain-containing protein -
  DTO10_RS12235 (DTO10_12235) ispD 2547790..2548470 (+) 681 WP_116821268.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  DTO10_RS12240 (DTO10_12240) ispF 2548488..2548967 (+) 480 WP_056521805.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -

Sequence

Protein


Download         Length: 462 a.a.        Molecular weight: 50189.67 Da        Isoelectric Point: 7.4846

>NTDB_id=302320 DTO10_RS12220 WP_098174999.1 2543980..2545368(+) (radA) [Peribacillus butanolivorans strain PHB-7a]
MAAKKKTKFMCQSCGYESAKWMGKCPGCGEWNKMVEETEIVKPARKGAFTHSEVRGSGEREKAAPITTIQSDKEPRIKTD
LMELNRALGGGIVQGSLVLIGGDPGIGKSTLLLQVSSQLAHKQKKVLYISGEESVKQTKLRADRLGDMSENLFVYAETDM
DYIQQAIQEVNPDLVIIDSIQTVYHSEVTSAPGSVSQVRECTASLMRIAKTNGIAIFIVGHVTKEGAIAGPRLLEHMVDT
VLYFEGERHHTYRIIRAVKNRFGSTNEMGIFEMKEHGLEEVANPSEIFLEERSQGASGSTVVASMEGTRPVLVEIQALIS
PTSFGNPRRMATGIDHNRVSLLMAVLEKRVGLLLQNQDAYLKVAGGVKLDEPAIDLAVAISIASSFRDKPTNPADCIIGE
VGLTGEVRRVSRIEQRVQEAAKLGFERVIIPANNIGGWTAPKGIKIVGVSSVSEALHHSLGG

Nucleotide


Download         Length: 1389 bp        

>NTDB_id=302320 DTO10_RS12220 WP_098174999.1 2543980..2545368(+) (radA) [Peribacillus butanolivorans strain PHB-7a]
ATGGCTGCAAAAAAGAAAACTAAGTTTATGTGTCAGTCTTGTGGGTATGAATCGGCGAAGTGGATGGGAAAATGCCCGGG
CTGTGGAGAATGGAATAAGATGGTCGAGGAAACCGAAATTGTTAAACCGGCTAGAAAAGGAGCTTTTACTCATTCAGAGG
TTAGAGGTTCCGGGGAACGTGAGAAGGCGGCACCTATAACTACTATTCAGTCAGACAAGGAACCTCGGATTAAGACAGAT
TTAATGGAATTGAATCGTGCGCTTGGTGGAGGGATTGTGCAGGGGTCACTTGTGTTAATTGGAGGAGATCCGGGTATTGG
TAAATCTACCCTACTTTTGCAAGTATCTTCACAGTTGGCGCATAAACAGAAAAAAGTGCTATATATTTCAGGTGAAGAAT
CAGTTAAACAGACCAAACTGAGAGCAGACCGGCTTGGGGATATGTCAGAAAATCTATTCGTTTATGCAGAAACTGATATG
GATTATATCCAACAGGCAATTCAAGAGGTAAATCCGGATTTAGTTATTATTGATTCAATTCAAACGGTGTATCATTCTGA
AGTGACTTCCGCGCCGGGAAGTGTTTCACAAGTAAGGGAATGTACGGCATCGCTCATGCGTATTGCGAAGACGAATGGAA
TTGCGATCTTTATCGTCGGGCATGTTACGAAAGAAGGGGCGATTGCAGGGCCAAGGTTGCTTGAGCATATGGTAGACACT
GTTTTATATTTCGAAGGAGAAAGACACCATACATATCGAATTATACGCGCGGTCAAAAACCGCTTTGGTTCGACAAATGA
GATGGGTATTTTTGAAATGAAGGAACATGGTCTAGAAGAAGTCGCGAATCCTTCAGAAATTTTCCTTGAGGAACGGTCAC
AAGGAGCTTCGGGATCTACCGTTGTTGCGTCAATGGAAGGAACAAGACCGGTGCTTGTCGAAATTCAGGCATTAATTTCA
CCGACGAGTTTTGGAAATCCAAGGCGAATGGCTACTGGAATTGATCACAATAGGGTCTCGCTTTTAATGGCGGTCTTGGA
AAAGAGAGTGGGTTTGTTATTACAAAACCAAGATGCGTACCTAAAGGTTGCTGGAGGTGTCAAGCTGGATGAACCGGCAA
TTGATCTGGCTGTGGCAATTAGCATTGCCTCAAGCTTCCGTGATAAACCAACAAATCCCGCCGATTGCATCATTGGAGAA
GTTGGGTTAACGGGGGAAGTAAGAAGGGTGTCCCGGATTGAACAACGAGTACAGGAAGCAGCAAAACTGGGGTTTGAACG
GGTCATTATACCCGCTAATAATATAGGTGGATGGACAGCTCCAAAGGGTATTAAGATCGTTGGGGTTTCATCGGTTTCGG
AGGCTCTTCATCATTCGTTAGGGGGGTAA

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(369-434)

AAA domain (AAA_25)

(77-226)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(9-36)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

77.874

99.784

0.777
  radA Streptococcus mitis NCTC 12261

62.335

98.268

0.613
  radA Streptococcus pneumoniae Rx1

62.335

98.268

0.613
  radA Streptococcus pneumoniae D39

62.335

98.268

0.613
  radA Streptococcus pneumoniae R6

62.335

98.268

0.613
  radA Streptococcus pneumoniae TIGR4

62.335

98.268

0.613
  radA Streptococcus mitis SK321

62.115

98.268

0.61

Multiple sequence alignment