Detailed information NTG0302152

Bioinformatically predicted

Overview

• Name: pilB
• Type: Machinery gene
• Locus tag: Ysp71_RS26680
• Genome accession: NZ_CP030911
• Coordinates: 5538268..5539968 (+)
• Length: 566 a.a.
• NCBI ID: WP_003120945.1
• Uniprot ID: A0A1L7HA63
• Organism: Pseudomonas aeruginosa strain Y71
• Function: power the assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 5533268..5544968

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
Ysp71_RS26645 (Ysp71_5120)	fliP	5533989..5534741 (+)	753	WP_023082025.1	flagellar type III secretion system pore protein FliP	-
Ysp71_RS26650 (Ysp71_5121)	fliQ	5534786..5535055 (+)	270	WP_023081841.1	flagellar biosynthesis protein FliQ	-
Ysp71_RS26655 (Ysp71_5122)	fliR	5535055..5535831 (+)	777	WP_003083054.1	flagellar biosynthetic protein FliR	-
Ysp71_RS26660	-	5535834..5536093 (+)	260	Protein_5158	EscU/YscU/HrcU family type III secretion system export apparatus switch protein	-
Ysp71_RS26665 (Ysp71_5124)	-	5537091..5537333 (+)	243	WP_023082027.1	hypothetical protein	-
Ysp71_RS26675	-	5537564..5538037 (-)	474	WP_015503553.1	pilin	-
Ysp71_RS26680 (Ysp71_5125)	pilB	5538268..5539968 (+)	1701	WP_003120945.1	type IV-A pilus assembly ATPase PilB	Machinery gene
Ysp71_RS26685 (Ysp71_5126)	pilC	5539972..5541189 (+)	1218	WP_010792214.1	type II secretion system F family protein	Machinery gene
Ysp71_RS26690 (Ysp71_5127)	pilD	5541192..5542064 (+)	873	WP_003116251.1	type IV prepilin peptidase/methyltransferase PilD	Machinery gene
Ysp71_RS26695 (Ysp71_5128)	coaE	5542061..5542672 (+)	612	WP_003112838.1	dephospho-CoA kinase	-
Ysp71_RS26700 (Ysp71_5129)	yacG	5542669..5542869 (+)	201	WP_003094656.1	DNA gyrase inhibitor YacG	-
Ysp71_RS26705 (Ysp71_5130)	-	5542906..5543115 (-)	210	WP_003120946.1	hypothetical protein	-
Ysp71_RS26710 (Ysp71_5131)	-	5543221..5543910 (-)	690	WP_003103868.1	energy-coupling factor ABC transporter permease	-
Ysp71_RS26715 (Ysp71_5132)	-	5543907..5544377 (-)	471	WP_019486073.1	hypothetical protein	-
Ysp71_RS26720 (Ysp71_5133)	-	5544374..5544799 (-)	426	WP_003103865.1	GNAT family N-acetyltransferase	-

Sequence

Protein

• Length: 566 a.a.
• Molecular weight: 62385.25 Da
• Isoelectric Point: 5.7055

>NTDB_id=302152 Ysp71_RS26680 WP_003120945.1 5538268..5539968(+) (pilB) [Pseudomonas aeruginosa strain Y71]
MNDSIQLSGLSRQLVQANLLDEKTAVQAQAQAQRNKLSLVTHLVQNKLVSGLALAELSAEQFGIAYCDLNSLDKESFPRD
AISEKLVRQHRVIPLWRRGNKLFVGISDPANHQAINDVQFSTGLTTEAILVEDDKLGLAIDKLFESATDGLAGLDDVDLE
GLDIGSAEKSTQEDTSAEADDAPVVRFVNKMLLDAIKGGSSDLHFEPYEKIYRVRFRTDGMLHEVAKPPIQLASRISARL
KVMAGLDISERRKPQDGRIKMRVSKSKSIDFRVNTLPTLWGEKIVMRILDSSSAQMGIDALGYEEDQKELYLAALKQPQG
MILVTGPTGSGKTVSLYTGLNILNTTDINISTAEDPVEINLEGINQVNVNPRQGMDFSQALRAFLRQDPDVIMVGEIRDL
ETAEIAIKAAQTGHMVMSTLHTNSAAETLTRLLNMGVPAFNLATSVNLIIAQRLARKLCSHCKKEHEVPRETLLHEGFPE
DKIGTFKLYSPVGCDHCKNGYKGRVGIYEVVKNTPALQRIIMEEGNSIEIAEQARKEGFNDLRTSGLLKAMQGITSLEEV
NRVTKD

Nucleotide

• Length: 1701 bp

>NTDB_id=302152 Ysp71_RS26680 WP_003120945.1 5538268..5539968(+) (pilB) [Pseudomonas aeruginosa strain Y71]
ATGAACGACAGCATCCAACTGAGCGGCCTGTCCCGACAGCTCGTCCAAGCCAATCTACTCGACGAAAAGACCGCCGTGCA
GGCCCAGGCGCAGGCGCAGCGCAACAAGCTGTCGCTGGTGACGCACCTGGTGCAGAACAAGCTGGTAAGCGGCCTGGCCC
TGGCCGAATTATCCGCCGAGCAGTTCGGTATCGCCTACTGCGACCTGAATAGCCTGGACAAGGAAAGCTTTCCGCGGGAC
GCCATCAGCGAAAAGCTGGTCCGCCAGCACCGGGTCATCCCTTTGTGGCGGCGCGGCAACAAGCTCTTCGTAGGCATCTC
CGATCCCGCCAACCACCAGGCCATCAATGATGTCCAGTTCAGCACCGGCCTGACGACCGAAGCGATTCTGGTCGAAGACG
ACAAGCTTGGCCTGGCCATCGACAAACTGTTCGAAAGCGCTACCGATGGTCTCGCAGGCCTCGACGATGTTGACCTCGAG
GGACTGGATATCGGTAGCGCGGAAAAGTCCACCCAGGAGGATACCAGCGCAGAAGCGGACGACGCTCCTGTAGTACGTTT
CGTCAACAAGATGCTGCTGGATGCCATAAAGGGTGGCTCTTCCGACCTGCACTTCGAGCCCTACGAGAAGATCTACCGGG
TGCGCTTCCGTACCGACGGCATGCTCCACGAAGTGGCCAAGCCGCCGATCCAGTTGGCCAGCCGTATTTCCGCGCGACTC
AAGGTGATGGCCGGCCTGGACATTTCCGAACGGCGCAAACCGCAGGACGGACGGATCAAGATGCGCGTGTCGAAGAGCAA
GTCCATCGACTTCCGCGTCAACACCCTGCCGACCCTGTGGGGCGAGAAGATCGTGATGCGGATCCTCGACTCCTCCAGCG
CACAAATGGGCATCGACGCCCTCGGCTACGAGGAGGACCAGAAGGAACTCTACCTGGCCGCACTCAAGCAGCCACAGGGC
ATGATCCTGGTCACCGGCCCCACCGGCTCGGGCAAGACGGTATCGCTATACACCGGCCTGAACATCCTCAATACCACCGA
CATCAACATTTCCACCGCCGAAGACCCGGTGGAGATCAACCTGGAAGGCATCAACCAGGTCAACGTCAACCCGCGCCAGG
GCATGGACTTCTCCCAGGCGCTGCGCGCCTTCCTGCGCCAGGACCCGGACGTGATCATGGTCGGCGAGATCCGCGACCTG
GAGACCGCCGAGATCGCCATCAAGGCGGCGCAGACCGGACATATGGTGATGTCCACCCTACACACCAACAGCGCCGCCGA
GACCCTGACTCGCCTGCTGAACATGGGCGTGCCGGCGTTCAACCTGGCGACCTCGGTGAACCTGATCATCGCCCAGCGCC
TTGCGCGAAAACTCTGTTCGCACTGCAAGAAAGAACACGAGGTGCCGAGGGAAACCCTGCTTCACGAGGGCTTTCCGGAA
GACAAGATCGGTACCTTCAAGCTTTATTCGCCGGTGGGCTGCGACCATTGCAAGAACGGTTACAAGGGCCGTGTCGGTAT
TTATGAAGTAGTTAAAAACACCCCGGCCCTGCAGCGGATTATCATGGAGGAAGGCAACTCCATCGAGATCGCCGAGCAAG
CCCGCAAAGAAGGCTTCAACGATCTGCGCACCTCAGGCCTGCTGAAAGCCATGCAGGGGATCACCAGCCTGGAGGAAGTC
AACCGCGTGACCAAGGATTAA

Domains

Predicted by InterproScan.

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (43-147)

• Type II/IV secretion system protein (T2SSE) (190-457)

3D structure

• AlphaFold DB: A0A1L7HA63

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilB	Acinetobacter baumannii D1279779	57.692	100	0.583
pilB	Acinetobacter baylyi ADP1	57.371	99.47	0.571
pilB	Legionella pneumophila strain ERS1305867	55.124	100	0.551
pilB	Vibrio parahaemolyticus RIMD 2210633	51.064	99.647	0.509
pilF	Neisseria gonorrhoeae MS11	50.712	99.293	0.504
pilB	Vibrio cholerae strain A1552	50.265	99.823	0.502
pilB	Vibrio campbellii strain DS40M4	49.823	99.647	0.496
pilF	Thermus thermophilus HB27	39.572	99.117	0.392
pilB	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	40.607	93.11	0.378
pilB/pilB1	Synechocystis sp. PCC 6803	38.563	93.463	0.36