Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   DS737_RS02615 Genome accession   NZ_CP030880
Coordinates   545576..546376 (+) Length   266 a.a.
NCBI ID   WP_026056343.1    Uniprot ID   A0AAW4JFL0
Organism   Acinetobacter haemolyticus strain HW-2A     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 540576..551376
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DS737_RS02590 - 541485..542801 (+) 1317 WP_228206839.1 glycosyltransferase family 1 protein -
  DS737_RS02595 - 542825..543376 (+) 552 WP_004640826.1 phosphatase PAP2 family protein -
  DS737_RS02600 iscX 543461..543661 (+) 201 WP_004640824.1 Fe-S cluster assembly protein IscX -
  DS737_RS02605 ndk 543771..544202 (+) 432 WP_004640823.1 nucleoside-diphosphate kinase -
  DS737_RS02610 rlmN 544323..545558 (+) 1236 WP_005083389.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  DS737_RS02615 pilF 545576..546376 (+) 801 WP_026056343.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  DS737_RS02620 - 546367..547161 (+) 795 WP_004640817.1 helix-turn-helix domain-containing protein -
  DS737_RS02625 ispG 547183..548298 (+) 1116 WP_113996690.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  DS737_RS02630 hisS 548295..549587 (+) 1293 WP_113996691.1 histidine--tRNA ligase -
  DS737_RS02635 - 549612..550310 (+) 699 WP_004640812.1 tetratricopeptide repeat protein -

Sequence

Protein


Download         Length: 266 a.a.        Molecular weight: 30220.21 Da        Isoelectric Point: 9.6050

>NTDB_id=302016 DS737_RS02615 WP_026056343.1 545576..546376(+) (pilF) [Acinetobacter haemolyticus strain HW-2A]
MNTTRLKTTIAITVVLMAGGLTACQSTPSTKDPEKAVQVRTQLAAEYIRTRDLDSAKRSLDQALKLNSRDANANMMMGIL
LQQEGSKLNMEKADAYFKRAISSEPDNAQARNNYGTYLYQMQRYNEAIEQFTRAGATLGYDQRFQSLENLGRIYLLLGDV
TNAEKSFKQALQANRNATISMLELSEIFYLQQNNRDASQLYEQYVRSVGQRNQGARALWIGIRIARANADQLGMQVLVNQ
LRALFPDSPEYQRYLQLQYSTEAVWK

Nucleotide


Download         Length: 801 bp        

>NTDB_id=302016 DS737_RS02615 WP_026056343.1 545576..546376(+) (pilF) [Acinetobacter haemolyticus strain HW-2A]
TTGAATACAACTCGACTAAAAACGACCATAGCGATAACAGTTGTACTTATGGCAGGTGGCTTGACAGCTTGCCAATCGAC
ACCATCTACGAAAGATCCAGAGAAAGCTGTTCAGGTTCGCACCCAATTGGCTGCGGAATATATTCGTACGCGTGATTTGG
ACTCTGCAAAACGTTCACTTGACCAAGCATTGAAACTTAATTCCCGTGATGCAAATGCCAATATGATGATGGGTATTTTG
CTGCAACAAGAGGGAAGTAAGCTCAATATGGAAAAAGCAGATGCTTATTTTAAGCGAGCGATTTCATCTGAGCCAGACAA
TGCTCAAGCGCGTAATAATTACGGTACATACTTATATCAGATGCAGCGTTATAATGAAGCAATTGAGCAGTTCACGCGTG
CTGGTGCAACCTTAGGTTATGATCAACGATTTCAATCATTGGAAAACCTAGGGCGAATCTATTTATTATTGGGCGATGTG
ACGAATGCTGAAAAATCATTCAAACAGGCGCTGCAAGCAAATCGAAATGCAACCATTTCAATGTTAGAGTTGTCGGAAAT
TTTCTATTTGCAACAAAACAATAGAGATGCATCCCAGTTATATGAACAATATGTTCGTAGCGTGGGACAGCGCAACCAAG
GTGCTAGAGCACTTTGGATCGGGATACGCATTGCTCGAGCGAATGCGGATCAGTTAGGAATGCAAGTGTTAGTCAACCAA
TTACGCGCTTTATTTCCTGATAGTCCAGAATATCAACGTTATTTGCAATTACAGTACAGTACTGAGGCCGTATGGAAATA
A

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_8)

(39-68)

Tetratricopeptide repeat (TPR_12)

(108-175)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

76.58

100

0.774
  pilF Acinetobacter baylyi ADP1

68.045

100

0.68

Multiple sequence alignment