Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   DAUD_RS03270 Genome accession   NC_010424
Coordinates   681929..682843 (+) Length   304 a.a.
NCBI ID   WP_012301768.1    Uniprot ID   B1I2E0
Organism   Candidatus Desulforudis audaxviator MP104C     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 676929..687843
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DAUD_RS03240 (Daud_0640) fabK 676983..677933 (+) 951 WP_012301762.1 enoyl-[acyl-carrier-protein] reductase FabK -
  DAUD_RS03245 (Daud_0641) fabD 677930..678871 (+) 942 WP_012301763.1 ACP S-malonyltransferase -
  DAUD_RS03250 (Daud_0642) fabG 678873..679616 (+) 744 WP_012301764.1 3-oxoacyl-[acyl-carrier-protein] reductase -
  DAUD_RS03255 (Daud_0643) - 679693..679926 (+) 234 WP_012301765.1 acyl carrier protein -
  DAUD_RS03260 (Daud_0644) fabF 679959..681212 (+) 1254 WP_012301766.1 beta-ketoacyl-ACP synthase II -
  DAUD_RS03265 (Daud_0645) rnc 681212..681919 (+) 708 WP_012301767.1 ribonuclease III -
  DAUD_RS03270 (Daud_0646) pilA 681929..682843 (+) 915 WP_012301768.1 signal recognition particle-docking protein FtsY Machinery gene
  DAUD_RS03275 (Daud_0647) mtnP 683163..684041 (+) 879 WP_012301769.1 S-methyl-5'-thioadenosine phosphorylase -
  DAUD_RS03280 (Daud_0648) mtnA 684041..685087 (+) 1047 WP_012301770.1 S-methyl-5-thioribose-1-phosphate isomerase -
  DAUD_RS03285 (Daud_0649) - 685084..686337 (+) 1254 WP_012301771.1 adenosylhomocysteinase -
  DAUD_RS03290 (Daud_0650) - 686460..687128 (+) 669 WP_012301772.1 class II aldolase/adducin family protein -

Sequence

Protein


Download         Length: 304 a.a.        Molecular weight: 32927.99 Da        Isoelectric Point: 6.7735

>NTDB_id=30163 DAUD_RS03270 WP_012301768.1 681929..682843(+) (pilA) [Candidatus Desulforudis audaxviator MP104C]
MGLFSRLKQSLTKTREVLAGKVEGLVYGRTTVDESLYEELEEILIQADVGVETSLELVARVRERMKAQKLDDPTKLKPIL
KDELRRIFAGGDETANLRLDGDPTVILVVGVNGTGKTTSIGKLAHLLRRDGKKVLLAAADTFRAAAIDQLEIWAHRAGVE
LIRHGEGADPAAVVYDAIQAARAREYDVLIIDTAGRLHTRVNLMEELKKVFRVVSRELPGAPQEVLLVLDATTGHNAVNQ
AKIFGEAVGVTGIVLTKLDGTAKGGIVAAIRNSQKIPVKLVGTGERIEDLSYFHAGEFVDGLLG

Nucleotide


Download         Length: 915 bp        

>NTDB_id=30163 DAUD_RS03270 WP_012301768.1 681929..682843(+) (pilA) [Candidatus Desulforudis audaxviator MP104C]
ATGGGCTTATTCAGCCGCCTGAAACAGAGCCTGACCAAGACCCGTGAGGTACTGGCCGGGAAGGTGGAGGGTCTGGTGTA
CGGGCGGACGACCGTAGACGAGTCCCTCTACGAGGAACTGGAAGAGATTTTGATTCAGGCCGACGTGGGGGTGGAGACTT
CCCTGGAGCTTGTGGCACGGGTGCGCGAGCGGATGAAGGCGCAGAAACTCGATGACCCCACCAAGCTCAAACCGATCCTC
AAGGATGAACTGCGCCGGATTTTCGCGGGCGGGGATGAAACCGCAAACCTTAGGCTGGACGGAGACCCGACGGTGATTCT
GGTGGTAGGAGTCAACGGGACGGGGAAGACGACCTCGATCGGCAAGCTGGCCCACCTGCTCCGCCGGGATGGAAAGAAAG
TGCTGCTGGCGGCGGCCGACACGTTCCGGGCGGCGGCGATCGACCAGTTGGAAATCTGGGCCCACAGGGCCGGGGTTGAG
TTGATCAGGCACGGTGAGGGGGCCGATCCGGCGGCAGTGGTTTACGACGCCATCCAGGCCGCCCGGGCGCGTGAGTACGA
CGTGTTGATCATCGACACGGCCGGTCGGCTGCACACCAGGGTCAACCTGATGGAAGAACTGAAAAAAGTGTTCCGGGTGG
TGAGCCGGGAACTGCCGGGTGCGCCTCAGGAAGTTTTGCTGGTTCTGGATGCGACCACCGGGCACAATGCGGTTAACCAG
GCCAAAATATTCGGTGAGGCCGTCGGGGTGACCGGAATCGTGCTGACCAAACTGGACGGAACCGCCAAGGGCGGAATCGT
GGCGGCGATCAGAAATTCGCAAAAGATACCAGTCAAACTGGTGGGCACCGGCGAGAGAATCGAAGACCTCTCCTATTTCC
ACGCCGGGGAGTTCGTAGACGGCCTGCTGGGATAA

Domains

Predicted by InterproScan.

SRP54-type protein, helical bundle domain (SRP54_N)

(20-84)

SRP54-type protein, GTPase domain (SRP54)

(103-303)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B1I2E0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Neisseria gonorrhoeae MS11

49.175

99.671

0.49

Multiple sequence alignment