Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   DPR03_RS11480 Genome accession   NZ_CP030754
Coordinates   2387683..2388483 (-) Length   266 a.a.
NCBI ID   WP_071320448.1    Uniprot ID   -
Organism   Acinetobacter schindleri strain H3     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2382683..2393483
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DPR03_RS11460 - 2383728..2384426 (-) 699 WP_004889998.1 YfgM family protein -
  DPR03_RS11465 hisS 2384457..2385752 (-) 1296 WP_071320447.1 histidine--tRNA ligase -
  DPR03_RS11470 ispG 2385756..2386877 (-) 1122 WP_008303409.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  DPR03_RS11475 - 2386901..2387692 (-) 792 WP_008303407.1 helix-turn-helix domain-containing protein -
  DPR03_RS11480 pilF 2387683..2388483 (-) 801 WP_071320448.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  DPR03_RS11485 rlmN 2388498..2389733 (-) 1236 WP_071320449.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  DPR03_RS11490 ndk 2389856..2390287 (-) 432 WP_004813112.1 nucleoside-diphosphate kinase -
  DPR03_RS11495 iscX 2390398..2390598 (-) 201 WP_004813114.1 Fe-S cluster assembly protein IscX -
  DPR03_RS11500 - 2390688..2391239 (-) 552 WP_048881427.1 phosphatase PAP2 family protein -
  DPR03_RS11505 - 2391309..2391719 (-) 411 WP_004890015.1 hypothetical protein -

Sequence

Protein


Download         Length: 266 a.a.        Molecular weight: 30161.37 Da        Isoelectric Point: 9.4227

>NTDB_id=301335 DPR03_RS11480 WP_071320448.1 2387683..2388483(-) (pilF) [Acinetobacter schindleri strain H3]
MRNLTSKIAFISTICVSIALSGCQTAEVGKKDPKKATQVRTQLAVEYLKTGDLDSAKRALDEALKINSRDAQANMMMGVL
LQQEGSRLNLDRAETYFKRAISAEPKNAQARNNYGTYLYQLERYNDAIEQFAIAGATLGYDQRYLALENMGRIYLKQGDL
VNAEKSFKQALQANRDSYISMLELAEIFYLNQQFPAASNMYQQFVRSVGQKNQGARALWIGIRTARAEGDQMGMQVLVNQ
LRALFPESPEYQRYLQLQYTTEAVWK

Nucleotide


Download         Length: 801 bp        

>NTDB_id=301335 DPR03_RS11480 WP_071320448.1 2387683..2388483(-) (pilF) [Acinetobacter schindleri strain H3]
TTGAGAAATCTGACATCTAAAATTGCTTTTATTTCAACAATATGTGTTTCTATTGCTCTATCTGGCTGTCAAACGGCAGA
AGTAGGTAAAAAGGATCCTAAAAAAGCGACCCAGGTGCGTACTCAATTGGCGGTTGAATATCTGAAGACTGGTGATCTGG
ATTCAGCGAAAAGAGCGCTAGATGAGGCGCTTAAAATTAATTCACGTGATGCTCAAGCCAATATGATGATGGGTGTTTTG
TTACAGCAGGAAGGTAGTAGGCTGAACTTGGATCGTGCCGAAACTTATTTTAAGCGCGCGATTTCAGCCGAACCTAAAAA
TGCACAGGCACGTAACAATTATGGCACCTATTTGTATCAGCTTGAACGTTATAATGATGCAATTGAACAGTTTGCAATTG
CAGGCGCGACACTAGGCTATGACCAGCGTTATCTTGCGCTTGAAAATATGGGGCGGATCTACCTGAAGCAGGGCGATCTG
GTGAATGCTGAAAAATCATTCAAACAGGCCCTGCAAGCCAATCGCGATTCTTACATTTCAATGTTAGAGTTGGCAGAGAT
TTTTTATCTGAATCAGCAGTTCCCGGCGGCGTCGAATATGTATCAACAATTTGTACGTTCAGTGGGACAGAAAAACCAGG
GTGCTCGTGCGCTCTGGATCGGCATTCGTACTGCGCGTGCCGAGGGTGATCAAATGGGCATGCAAGTATTAGTCAATCAA
TTGCGCGCATTATTCCCTGAAAGCCCGGAATACCAACGTTATTTGCAATTACAGTACACTACTGAGGCCGTATGGAAGTA
A

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_8)

(39-68)

Tetratricopeptide repeat (TPR_2)

(146-176)

Tetratricopeptide repeat (TPR_16)

(89-133)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

76.894

99.248

0.763
  pilF Acinetobacter baylyi ADP1

67.816

98.12

0.665

Multiple sequence alignment