Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   DRF63_RS04670 Genome accession   NZ_CP030753
Coordinates   1026134..1027528 (-) Length   464 a.a.
NCBI ID   WP_005597396.1    Uniprot ID   Q6XFB1
Organism   Actinobacillus pleuropneumoniae strain S4074     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1021134..1032528
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DRF63_RS04640 (DRF63_04635) lptE 1021748..1022242 (+) 495 WP_005597385.1 LPS assembly lipoprotein LptE -
  DRF63_RS04645 (DRF63_04640) holA 1022351..1023376 (+) 1026 WP_005612206.1 DNA polymerase III subunit delta -
  DRF63_RS04650 (DRF63_04645) yacG 1023453..1023641 (-) 189 WP_005597388.1 DNA gyrase inhibitor YacG -
  DRF63_RS04655 (DRF63_04650) coaE 1023623..1024261 (-) 639 WP_005597390.1 dephospho-CoA kinase -
  DRF63_RS04660 (DRF63_04655) - 1024283..1024921 (-) 639 WP_005597392.1 prepilin peptidase -
  DRF63_RS04665 (DRF63_04660) pilC 1024948..1026150 (-) 1203 WP_005597394.1 type II secretion system F family protein Machinery gene
  DRF63_RS04670 (DRF63_04665) pilB 1026134..1027528 (-) 1395 WP_005597396.1 GspE/PulE family protein Machinery gene
  DRF63_RS04675 (DRF63_04670) pilA 1027546..1027992 (-) 447 WP_005597397.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  DRF63_RS04680 (DRF63_04675) radA 1028174..1029553 (+) 1380 WP_005597398.1 DNA repair protein RadA -
  DRF63_RS04685 (DRF63_04680) - 1029597..1029830 (-) 234 WP_005597399.1 YgjV family protein -
  DRF63_RS04690 (DRF63_04685) folA 1029957..1030445 (-) 489 WP_005597400.1 type 3 dihydrofolate reductase -
  DRF63_RS04695 (DRF63_04690) proB 1030532..1031623 (+) 1092 WP_005597401.1 glutamate 5-kinase -
  DRF63_RS04700 (DRF63_04695) - 1031636..1032412 (+) 777 WP_005597402.1 TSUP family transporter -

Sequence

Protein


Download         Length: 464 a.a.        Molecular weight: 53056.91 Da        Isoelectric Point: 6.5042

>NTDB_id=301281 DRF63_RS04670 WP_005597396.1 1026134..1027528(-) (pilB) [Actinobacillus pleuropneumoniae strain S4074]
MQYSVCDVNNRQVFRISEEQWQKNYDERHILLRYLTVPLREDQQTLWLAIDDMNNLSACEIFAFLTHKRIEPVLVSSEEL
KYLLGQLMPEQQSVYDETELPYQPLAQAQHSDVNDPIIQVLDGLFKYGLKHNASDIHFEPQKDKMLIRLRIDGVLHPYKS
FSLQLANRLISRIKLLAKLDISESRLPQDGQFGFKTILSETLDFRVSSLPTYWGEKVVLRLQKNKPTHFDFTTLGFLAEQ
KEKLLRALRQPQGLILVTGPTGSGKSITLYSALDYLNDSKRHILTAEDPIEIELEGIIQTQINPAIDLGFSRLLRTFLRQ
DPDVIMLGEIRDQESAEIALSAAQTGHLVLSTLHTNDAPSAIERLLQLGIKEYEINNSLLLVVAQRLVRKLCTECGGKGC
KYCYHGYRGRIGVYQLFSKNGKIFEKSTACLDFENLYQSAQQKFKTGITSLEEIERVLGHVESV

Nucleotide


Download         Length: 1395 bp        

>NTDB_id=301281 DRF63_RS04670 WP_005597396.1 1026134..1027528(-) (pilB) [Actinobacillus pleuropneumoniae strain S4074]
ATGCAGTATTCCGTATGTGATGTAAACAATCGGCAAGTATTCCGGATTTCCGAGGAACAGTGGCAAAAAAATTATGATGA
ACGTCATATCTTATTACGTTATCTCACCGTACCGCTTCGGGAAGACCAACAAACATTATGGTTGGCAATCGATGATATGA
ATAATCTCAGTGCTTGCGAGATTTTTGCTTTTCTTACGCATAAGCGAATTGAGCCGGTATTGGTCTCTTCCGAAGAATTG
AAATACCTGCTCGGGCAATTAATGCCGGAACAACAATCGGTATATGACGAAACGGAATTACCTTATCAACCTTTAGCACA
AGCGCAGCATTCGGATGTTAATGATCCGATTATTCAAGTGTTGGACGGCCTGTTTAAATACGGTTTAAAACATAATGCTT
CGGATATTCATTTTGAACCGCAAAAAGACAAAATGTTGATTCGATTACGTATCGACGGCGTGTTACATCCGTATAAATCA
TTTTCTCTCCAACTTGCCAATCGACTTATTTCTCGCATTAAATTATTAGCTAAACTGGATATTAGTGAATCCCGTCTTCC
GCAAGACGGGCAATTCGGTTTTAAGACAATCTTATCCGAAACATTGGATTTCCGTGTTTCGAGTTTACCGACTTATTGGG
GCGAAAAAGTCGTTTTACGGCTACAAAAAAATAAACCGACGCATTTTGATTTTACTACGTTGGGCTTCTTGGCGGAGCAG
AAGGAAAAGTTGTTGCGGGCATTGCGGCAGCCTCAAGGATTAATATTAGTTACGGGACCGACCGGCAGTGGTAAAAGTAT
TACCTTATATAGTGCGTTGGATTATTTAAATGACAGTAAAAGGCATATTTTGACCGCCGAAGATCCGATAGAAATCGAAC
TTGAAGGCATAATCCAAACGCAAATAAACCCGGCGATAGATTTAGGGTTCAGCCGGCTATTAAGAACGTTTTTACGACAA
GATCCGGATGTGATTATGTTAGGTGAAATTCGTGATCAAGAAAGTGCCGAAATTGCATTAAGTGCGGCACAAACCGGGCA
TTTAGTGCTTTCGACCTTACATACCAATGATGCGCCTTCGGCAATCGAACGCTTATTGCAGCTCGGTATCAAAGAATATG
AAATCAATAATTCGTTATTACTTGTCGTGGCGCAACGTTTAGTTCGTAAATTATGTACCGAATGCGGCGGAAAAGGCTGT
AAATATTGTTATCACGGCTACAGAGGCAGAATCGGCGTTTATCAACTGTTTTCAAAAAATGGAAAAATTTTTGAGAAATC
GACCGCTTGTTTGGATTTTGAAAACTTATATCAAAGTGCGCAACAGAAATTCAAGACGGGAATCACTTCATTAGAAGAAA
TCGAGAGAGTGTTAGGTCATGTTGAAAGTGTATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(129-390)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(28-92)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q6XFB1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Glaesserella parasuis strain SC1401

68.192

98.922

0.675
  pilB Haemophilus influenzae Rd KW20

58.499

97.629

0.571
  pilB Haemophilus influenzae 86-028NP

58.057

97.629

0.567
  pilB Vibrio campbellii strain DS40M4

40.333

100

0.418
  pilB Legionella pneumophila strain ERS1305867

39.468

100

0.416
  pilB Vibrio parahaemolyticus RIMD 2210633

39.917

100

0.414
  pilB Acinetobacter baumannii D1279779

39.059

100

0.412
  pilB Vibrio cholerae strain A1552

39.212

100

0.407
  pilB Acinetobacter baylyi ADP1

38.525

100

0.405
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

36.538

100

0.369

Multiple sequence alignment