Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   DRF63_RS04665 Genome accession   NZ_CP030753
Coordinates   1024948..1026150 (-) Length   400 a.a.
NCBI ID   WP_005597394.1    Uniprot ID   Q6XFB0
Organism   Actinobacillus pleuropneumoniae strain S4074     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1019948..1031150
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DRF63_RS04640 (DRF63_04635) lptE 1021748..1022242 (+) 495 WP_005597385.1 LPS assembly lipoprotein LptE -
  DRF63_RS04645 (DRF63_04640) holA 1022351..1023376 (+) 1026 WP_005612206.1 DNA polymerase III subunit delta -
  DRF63_RS04650 (DRF63_04645) yacG 1023453..1023641 (-) 189 WP_005597388.1 DNA gyrase inhibitor YacG -
  DRF63_RS04655 (DRF63_04650) coaE 1023623..1024261 (-) 639 WP_005597390.1 dephospho-CoA kinase -
  DRF63_RS04660 (DRF63_04655) - 1024283..1024921 (-) 639 WP_005597392.1 prepilin peptidase -
  DRF63_RS04665 (DRF63_04660) pilC 1024948..1026150 (-) 1203 WP_005597394.1 type II secretion system F family protein Machinery gene
  DRF63_RS04670 (DRF63_04665) pilB 1026134..1027528 (-) 1395 WP_005597396.1 GspE/PulE family protein Machinery gene
  DRF63_RS04675 (DRF63_04670) pilA 1027546..1027992 (-) 447 WP_005597397.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  DRF63_RS04680 (DRF63_04675) radA 1028174..1029553 (+) 1380 WP_005597398.1 DNA repair protein RadA -
  DRF63_RS04685 (DRF63_04680) - 1029597..1029830 (-) 234 WP_005597399.1 YgjV family protein -
  DRF63_RS04690 (DRF63_04685) folA 1029957..1030445 (-) 489 WP_005597400.1 type 3 dihydrofolate reductase -

Sequence

Protein


Download         Length: 400 a.a.        Molecular weight: 45588.11 Da        Isoelectric Point: 10.2885

>NTDB_id=301280 DRF63_RS04665 WP_005597394.1 1024948..1026150(-) (pilC) [Actinobacillus pleuropneumoniae strain S4074]
MLKVYEFHWKAKNRFQQLQKGKALACHRTELERRLLAKGYSQIRIQRNFRFVTQASSESITQVIKQLALLLNAAVPLKQA
LNMLLENTYHIRIYMWLNDLNRLIESGYTMSSSLTKLNLYLAKSEIQLVRIGEQSGRLGEILQNIAVNRYQTEKLHKKIK
KIMFYPVVTLIISVALSLAMLLFIVPQFIELYSGREKPLPLITQMLFTLSLFLQHSLLQVTIVSLLAVGFFRFFAKKYRF
LTAIKNGVLSALPIFNKIVENVRIVFFSQNLGLMLKAHIRLETALNTFLATQSQDLRLQKEIENILAQLKQGYRFSESIN
ASVFGTQVVQMLAIGEQSGNVAQMCSYIGELYRQQLDDQIELLSQLLEPILMLIIGLIIGTVLIGLYLPIFDLGGLVGGG

Nucleotide


Download         Length: 1203 bp        

>NTDB_id=301280 DRF63_RS04665 WP_005597394.1 1024948..1026150(-) (pilC) [Actinobacillus pleuropneumoniae strain S4074]
ATGTTGAAAGTGTATGAATTCCATTGGAAAGCTAAAAATCGTTTTCAACAACTTCAGAAAGGAAAAGCGTTAGCTTGTCA
TCGAACGGAATTGGAACGGCGTTTATTAGCAAAAGGCTATTCACAGATCCGTATCCAACGTAATTTTCGTTTTGTCACAC
AGGCTTCTTCAGAATCGATTACTCAAGTAATTAAACAGTTGGCGCTTTTACTGAATGCCGCAGTCCCTTTAAAACAAGCG
CTGAATATGTTGTTAGAAAATACTTATCATATTCGGATCTATATGTGGCTGAATGATTTAAACCGTTTGATAGAATCGGG
TTATACAATGTCGTCAAGTTTAACCAAATTGAATTTATATTTGGCGAAATCGGAAATACAGCTGGTACGGATTGGCGAAC
AAAGCGGGCGATTAGGCGAAATTTTGCAAAATATTGCGGTAAATCGCTATCAAACCGAAAAATTACATAAGAAAATAAAA
AAGATAATGTTTTATCCTGTCGTCACTTTGATTATTTCCGTTGCGCTTTCTCTCGCCATGCTCCTTTTTATCGTGCCGCA
ATTTATCGAGCTGTATTCCGGTCGTGAAAAGCCGCTTCCTTTAATTACACAAATGCTATTTACATTATCGCTCTTCTTAC
AACATTCTTTATTACAGGTAACGATAGTCAGTTTATTAGCGGTCGGTTTTTTCCGTTTTTTTGCAAAAAAATATCGATTT
TTAACCGCTATAAAAAACGGGGTGTTAAGTGCTTTGCCTATTTTTAATAAGATAGTCGAAAACGTTCGTATAGTATTTTT
TAGTCAAAATCTCGGCTTGATGCTAAAAGCTCATATTCGGCTGGAAACGGCGTTAAATACCTTTCTTGCTACACAATCGC
AAGATTTGAGGTTACAAAAAGAGATAGAAAATATTTTAGCGCAATTAAAACAAGGTTATCGCTTTTCCGAAAGTATTAAT
GCGAGTGTGTTCGGTACTCAAGTAGTACAAATGCTTGCCATTGGCGAACAGAGTGGGAATGTAGCGCAGATGTGTAGCTA
TATCGGGGAGTTATATCGACAACAATTGGATGATCAGATTGAACTGTTATCGCAGCTGCTTGAGCCGATCTTAATGCTGA
TTATCGGATTAATTATCGGTACGGTTTTGATCGGATTATATTTACCGATTTTTGATTTAGGCGGACTAGTCGGAGGCGGT
TAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(267-389)

Type II secretion system (T2SS), protein F (T2SSF)

(64-186)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q6XFB0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Glaesserella parasuis strain SC1401

49.874

99.25

0.495
  pilC Haemophilus influenzae Rd KW20

40

100

0.4
  pilC Haemophilus influenzae 86-028NP

39

100

0.39

Multiple sequence alignment