Detailed information    

insolico Bioinformatically predicted

Overview

Name   recJ   Type   Machinery gene
Locus tag   ETT72_RS04455 Genome accession   NZ_CP035427
Coordinates   827186..829396 (+) Length   736 a.a.
NCBI ID   WP_032464327.1    Uniprot ID   -
Organism   Streptococcus pyogenes strain emm74     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 822186..834396
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ETT72_RS04425 (ETT72_04420) - 822333..822509 (-) 177 WP_021299313.1 DUF3042 family protein -
  ETT72_RS04430 (ETT72_04425) miaA 822643..823542 (+) 900 WP_032464324.1 tRNA (adenosine(37)-N6)-dimethylallyltransferase MiaA -
  ETT72_RS04435 (ETT72_04430) hflX 823615..824853 (+) 1239 WP_032464325.1 GTPase HflX -
  ETT72_RS04440 (ETT72_04435) - 824846..825481 (+) 636 WP_032464326.1 cystathionine beta-lyase -
  ETT72_RS04445 (ETT72_04440) rnz 825496..826425 (+) 930 WP_009881223.1 ribonuclease Z -
  ETT72_RS04450 (ETT72_04445) - 826425..827189 (+) 765 WP_002984893.1 SDR family oxidoreductase -
  ETT72_RS04455 (ETT72_04450) recJ 827186..829396 (+) 2211 WP_032464327.1 single-stranded-DNA-specific exonuclease RecJ Machinery gene
  ETT72_RS04460 (ETT72_04455) - 829546..830064 (+) 519 WP_020833433.1 adenine phosphoribosyltransferase -
  ETT72_RS04465 (ETT72_04460) - 830145..830828 (+) 684 WP_002984890.1 DnaD domain-containing protein -
  ETT72_RS04470 (ETT72_04465) nth 830825..831481 (+) 657 WP_032464328.1 endonuclease III -
  ETT72_RS04475 (ETT72_04470) - 831553..832239 (+) 687 WP_002990104.1 tRNA (adenine(22)-N(1))-methyltransferase TrmK -
  ETT72_RS04480 (ETT72_04475) - 832229..833017 (+) 789 WP_002984887.1 Nif3-like dinuclear metal center hexameric protein -
  ETT72_RS04485 (ETT72_04480) - 833057..834163 (+) 1107 WP_032464329.1 FAD-binding oxidoreductase -

Sequence

Protein


Download         Length: 736 a.a.        Molecular weight: 82560.40 Da        Isoelectric Point: 4.7856

>NTDB_id=300896 ETT72_RS04455 WP_032464327.1 827186..829396(+) (recJ) [Streptococcus pyogenes strain emm74]
MIKSKYSWKIKDKKPDDGFFKLAKTKGLTQTAAQLIYERGIRTEEALDEFLTADLSQLHDPYLLHDMAKAVPRIRQAIEE
GERILIYGDYDADGMTSASIVKETLDMMGAEPLVYLPNRFTDGYGPNQSVYKYFIEQEAVSLIITVDNGVAGHEAIRYAQ
EQEVDVIVTDHHSLPEELPEAFAIIHPEHPDADYPFKHLAGCGVAFKLATALLESLPTDCLDLVAIGTIADMVSLTGENR
VLVKNGLAMLKHTERVGLQELMSLSPIDLEHFNEDAIGFQIAPQLNALGRLDDPNPAIELLTGFDDQEAQAIALMIKKKN
EERKALVQDIFDQAMAMVDPQKPVQVLAQAGWHPGVLGIVAGRIMETIGQTVVVLTIDNGFAKGSARSLEAINIFEALNG
KRELFTAFGGHAGAAGMTLPVDNLEALSDFLCQFVIEHGLDQTAKNTLTIDERLSLDDLSLDILKSLDKLAPYGMDHQKP
VFYVKDIRVSQARTIGQDQSHLKFKVSQGKASFDVLAFGQGSQLQEFRQTTGLELAVTLSVNHWNGNTSLQFMLVDARVD
GVQLLDLRTKTAKVPEGIPTIEEDPNARVILINDIPEDFKAWRNQFVHKDFDAIYFKNQMKHPYYLTGFGSREQFAKLYK
TIYQFPEFDLRHKLTELSHYLNIEKLLLIKLIQIFEELSFVTIDDGLMTVNPQAQKREISESHIYQDLKELVKFQEIMAL
ASPKEMYDYLVKSNDQ

Nucleotide


Download         Length: 2211 bp        

>NTDB_id=300896 ETT72_RS04455 WP_032464327.1 827186..829396(+) (recJ) [Streptococcus pyogenes strain emm74]
ATGATTAAATCCAAATATAGCTGGAAAATAAAAGACAAGAAGCCAGATGATGGCTTCTTTAAGCTAGCAAAGACAAAAGG
TCTAACCCAGACCGCCGCTCAGTTGATTTATGAGCGAGGGATAAGGACTGAAGAAGCCTTAGATGAGTTTTTAACAGCGG
ACTTGTCTCAACTGCATGATCCTTACTTGCTACATGACATGGCAAAAGCTGTGCCAAGAATTCGTCAAGCTATTGAAGAG
GGCGAACGAATTCTCATTTACGGCGACTATGATGCTGATGGCATGACGTCGGCTTCTATTGTCAAAGAAACCCTAGACAT
GATGGGAGCAGAGCCTTTGGTTTATCTTCCCAACCGCTTTACAGATGGCTACGGCCCCAACCAGAGCGTCTATAAATATT
TTATTGAGCAGGAAGCTGTGTCCCTAATTATCACGGTTGATAATGGCGTGGCCGGACATGAAGCGATTAGATATGCTCAA
GAACAGGAAGTTGATGTCATTGTAACTGACCATCATAGCCTGCCTGAAGAATTGCCAGAAGCCTTTGCCATCATTCATCC
TGAGCATCCTGATGCTGATTATCCCTTTAAACACTTAGCTGGCTGTGGCGTTGCCTTTAAATTGGCAACAGCTTTGCTAG
AAAGTTTACCAACAGACTGTCTAGACTTGGTAGCTATTGGAACCATTGCCGATATGGTAAGCTTGACAGGTGAAAACCGT
GTTTTAGTCAAAAATGGTCTGGCCATGCTCAAACACACCGAAAGAGTGGGCTTGCAGGAGTTGATGTCCTTGTCTCCTAT
TGATTTGGAACATTTTAATGAAGACGCCATTGGCTTTCAGATTGCTCCGCAATTAAATGCTTTAGGGCGTTTAGATGACC
CTAATCCAGCCATTGAGCTACTGACGGGCTTTGATGACCAAGAAGCTCAAGCGATTGCCTTGATGATTAAGAAAAAAAAT
GAAGAGCGTAAGGCCTTAGTCCAAGACATTTTTGATCAAGCGATGGCTATGGTTGATCCTCAAAAACCTGTTCAAGTCTT
GGCACAAGCTGGCTGGCATCCTGGTGTTCTGGGGATTGTCGCTGGTCGTATCATGGAAACTATTGGACAAACGGTCGTCG
TTTTGACCATTGATAATGGCTTTGCAAAAGGCTCGGCCAGAAGTTTAGAAGCTATTAATATTTTTGAAGCCTTAAATGGC
AAGCGTGAGTTATTTACAGCCTTTGGGGGACATGCTGGTGCAGCAGGAATGACCCTGCCCGTTGACAATTTGGAAGCTCT
CTCAGACTTTCTTTGTCAGTTTGTGATAGAACATGGCTTAGATCAGACTGCCAAAAATACGTTAACTATTGATGAAAGAC
TGTCTTTGGACGACCTTAGCCTTGATATTTTAAAGAGTTTAGATAAATTAGCCCCTTATGGGATGGATCATCAAAAGCCG
GTCTTTTATGTCAAGGACATAAGGGTTAGTCAAGCGCGTACCATAGGGCAAGACCAGAGCCATCTTAAATTTAAAGTCAG
TCAGGGCAAAGCTTCCTTTGACGTACTGGCTTTTGGTCAAGGAAGTCAACTGCAAGAGTTCCGACAAACTACTGGTTTAG
AATTAGCAGTTACCTTATCTGTCAACCATTGGAATGGGAACACGAGTCTCCAATTCATGTTAGTAGATGCTCGTGTTGAC
GGAGTGCAACTTCTTGACTTAAGGACAAAAACAGCCAAAGTTCCTGAAGGTATTCCGACTATTGAAGAAGACCCAAATGC
CAGAGTCATTCTGATTAATGACATCCCTGAAGATTTCAAAGCCTGGCGCAACCAGTTTGTTCACAAGGATTTTGACGCCA
TCTATTTTAAAAATCAGATGAAACATCCTTATTATTTAACAGGATTTGGCAGCAGAGAGCAGTTTGCCAAGCTCTACAAA
ACCATCTATCAGTTTCCTGAATTTGATTTACGACATAAATTGACAGAGTTGAGTCACTATTTAAACATAGAAAAACTTTT
ACTGATTAAGTTGATTCAAATTTTTGAAGAGTTGTCTTTTGTGACGATTGACGATGGGCTGATGACGGTTAACCCCCAAG
CGCAAAAACGAGAGATTTCAGAGAGCCACATTTACCAAGACTTAAAAGAATTGGTGAAATTTCAAGAAATCATGGCTTTG
GCTAGCCCGAAAGAGATGTATGATTATTTGGTGAAATCAAACGATCAATAA

Domains

Predicted by InterProScan.

Single-strand DNA-specific exonuclease, C terminal domain (ssDNA-exonuc_C)

(588-729)

DHHA1 domain (DHHA1)

(317-435)

RecJ OB domain (RecJ_OB)

(450-556)

DHH family (DHH)

(83-228)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recJ Streptococcus pneumoniae strain D39V

63.279

100

0.635
  recJ Bacillus subtilis subsp. subtilis str. 168

34.866

100

0.371