Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   DQ228_RS08985 Genome accession   NZ_CP030250
Coordinates   1753409..1754770 (-) Length   453 a.a.
NCBI ID   WP_024704235.1    Uniprot ID   -
Organism   Streptococcus thermophilus strain CS20     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1748409..1759770
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DQ228_RS08960 rplA 1750154..1750843 (-) 690 WP_014608720.1 50S ribosomal protein L1 -
  DQ228_RS08965 rplK 1750943..1751368 (-) 426 WP_002953535.1 50S ribosomal protein L11 -
  DQ228_RS08970 - 1751540..1751893 (+) 354 WP_002953536.1 DUF3397 domain-containing protein -
  DQ228_RS08975 - 1751985..1752482 (-) 498 WP_024704233.1 beta-class carbonic anhydrase -
  DQ228_RS08980 - 1752624..1753320 (-) 697 Protein_1755 TIGR00266 family protein -
  DQ228_RS08985 radA 1753409..1754770 (-) 1362 WP_024704235.1 DNA repair protein RadA Machinery gene
  DQ228_RS08990 - 1754948..1755394 (-) 447 WP_024010084.1 dUTP diphosphatase -
  DQ228_RS08995 - 1755608..1756210 (+) 603 Protein_1758 NADPH-dependent FMN reductase -
  DQ228_RS09000 - 1756229..1757480 (+) 1252 Protein_1759 NAD(P)H-dependent oxidoreductase -
  DQ228_RS09005 - 1757598..1758388 (+) 791 Protein_1760 formate/nitrite transporter family protein -
  DQ228_RS09010 - 1758397..1758970 (+) 574 Protein_1761 MptD family putative ECF transporter S component -

Sequence

Protein


Download         Length: 453 a.a.        Molecular weight: 49388.78 Da        Isoelectric Point: 6.6528

>NTDB_id=300446 DQ228_RS08985 WP_024704235.1 1753409..1754770(-) (radA) [Streptococcus thermophilus strain CS20]
MAKKKSTFVCRECGYNSPKYLGRCPNCSSWSSFEEEVEVQEVKNARVSLAGEKSKPTKLKEVSSISYARIKTDMDEFNRV
LGGGVVPGSLVLIGGDPGIGKSTLLLQVSTQLANKGTVLYVSGEESAEQIKLRSERLGDIDNEFYLYAETNMQAVRSQVE
GIKPDFLIIDSIQTIMSPEISGVQGSVSQVREVTAELIQIAKTNNIATFIVGHVTKEGQLAGPRMLEHMVDTVLYFEGER
HHTFRILRAVKNRFGSTNEIGIFEMQSGGLVEVLNPSQVFLEERLDGATGSAIVVTMEGSRPILAEVQALVTPTVFGNAK
RTTTGLDFNRVSLIMAVLEKRCGLLLQNQDAYLKSAGGVKLDEPAIDLAVAVAIASSYKELPTNPQEAFVGEIGLTGEIR
RVTRIEQRINEAAKLGFTKIYVPKNSLHGMKIPEGIQVIGVTTVGEVLKKVFS

Nucleotide


Download         Length: 1362 bp        

>NTDB_id=300446 DQ228_RS08985 WP_024704235.1 1753409..1754770(-) (radA) [Streptococcus thermophilus strain CS20]
ATAGCTAAGAAAAAATCAACTTTTGTTTGTAGAGAATGTGGCTACAATTCACCCAAATATCTAGGGCGCTGTCCCAATTG
TTCTTCTTGGTCCTCTTTTGAGGAAGAGGTTGAGGTACAGGAGGTTAAAAATGCCCGTGTCAGTTTGGCGGGTGAAAAGT
CCAAACCTACTAAGTTAAAAGAAGTCTCTTCAATTAGTTATGCCCGCATAAAAACCGATATGGACGAGTTTAACCGTGTT
TTAGGTGGCGGTGTGGTTCCGGGGAGTTTGGTTCTTATTGGAGGAGATCCAGGAATAGGGAAATCGACCCTCCTCTTACA
GGTGTCAACACAGCTGGCTAACAAGGGAACTGTCCTCTATGTTTCTGGGGAAGAATCGGCCGAGCAAATCAAATTGCGGA
GTGAACGTTTAGGTGATATTGATAACGAATTTTATCTGTATGCTGAGACCAATATGCAAGCAGTCCGTAGTCAAGTGGAG
GGTATCAAACCCGATTTCCTCATTATTGATTCTATTCAGACCATCATGAGCCCAGAGATCTCTGGCGTTCAAGGTTCTGT
TTCCCAGGTTCGTGAAGTAACTGCCGAACTCATACAGATTGCCAAAACCAATAACATTGCCACTTTTATTGTTGGGCATG
TGACCAAGGAGGGACAGCTGGCTGGACCACGTATGTTGGAGCACATGGTGGATACTGTGCTTTATTTTGAGGGGGAACGT
CATCATACCTTCCGTATTCTGAGGGCAGTTAAAAACCGTTTTGGTTCAACCAATGAGATTGGTATTTTTGAAATGCAGTC
AGGTGGTCTGGTCGAGGTACTAAACCCTAGCCAAGTTTTCTTAGAAGAACGCCTAGACGGTGCCACAGGTTCTGCCATTG
TTGTCACAATGGAGGGGAGTCGACCTATCCTAGCTGAGGTTCAAGCTTTGGTGACACCGACCGTCTTTGGTAACGCAAAG
CGGACAACGACGGGACTGGACTTTAACCGTGTCAGCCTTATCATGGCTGTCTTAGAGAAACGTTGCGGCCTTCTTCTCCA
AAATCAAGATGCCTATCTCAAATCAGCTGGTGGTGTCAAGTTGGACGAGCCAGCTATTGACCTTGCAGTTGCCGTTGCTA
TCGCTTCTAGTTACAAGGAGCTTCCAACCAATCCACAAGAAGCCTTCGTAGGAGAGATTGGCTTGACCGGAGAGATTCGT
CGTGTGACTCGTATTGAGCAGCGTATCAATGAAGCCGCTAAACTAGGTTTTACTAAGATTTATGTTCCTAAAAATTCGCT
TCATGGCATGAAGATTCCAGAAGGTATCCAGGTCATTGGGGTCACAACGGTAGGAGAAGTACTCAAAAAAGTCTTTTCTT
AA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(61-218)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Subunit ChlI of Mg-chelatase (ChlI)

(360-425)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus pneumoniae Rx1

88.742

100

0.887
  radA Streptococcus pneumoniae D39

88.742

100

0.887
  radA Streptococcus pneumoniae R6

88.742

100

0.887
  radA Streptococcus pneumoniae TIGR4

88.742

100

0.887
  radA Streptococcus mitis NCTC 12261

88.742

100

0.887
  radA Streptococcus mitis SK321

88.742

100

0.887
  radA Bacillus subtilis subsp. subtilis str. 168

61.148

100

0.611

Multiple sequence alignment