Detailed information NTG0300430

Bioinformatically predicted

Overview

• Name: endA
• Type: Machinery gene
• Locus tag: DQ228_RS05605
• Genome accession: NZ_CP030250
• Coordinates: 1084007..1084888 (-)
• Length: 293 a.a.
• NCBI ID: WP_084828863.1
• Uniprot ID: A0A0E2QH56
• Organism: Streptococcus thermophilus strain CS20
• Function: cleavage of dsDNA into ssDNA (predicted from homology) DNA processing

Genomic Context

Location: 1079007..1089888

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
DQ228_RS05580	vicR	1079712..1080419 (-)	708	WP_011681206.1	response regulator YycF	Regulator
DQ228_RS05585	-	1080946..1081713 (+)	768	WP_011681207.1	amino acid ABC transporter ATP-binding protein	-
DQ228_RS05590	-	1081725..1082558 (+)	834	WP_011681208.1	transporter substrate-binding domain-containing protein	-
DQ228_RS05595	-	1082576..1083274 (+)	699	WP_014608374.1	amino acid ABC transporter permease	-
DQ228_RS05600	-	1083286..1083936 (+)	651	WP_014608375.1	amino acid ABC transporter permease	-
DQ228_RS05605	endA	1084007..1084888 (-)	882	WP_084828863.1	DNA/RNA non-specific endonuclease	Machinery gene
DQ228_RS05610	-	1084950..1085138 (-)	189	WP_011226090.1	DNA-directed RNA polymerase subunit beta	-
DQ228_RS05615	murA	1085140..1086411 (-)	1272	WP_082308982.1	UDP-N-acetylglucosamine 1-carboxyvinyltransferase	-
DQ228_RS05620	-	1086481..1086717 (-)	237	WP_002946959.1	DUF1146 family protein	-
DQ228_RS05625	galE	1086905..1087918 (-)	1014	WP_014621668.1	UDP-glucose 4-epimerase GalE	-
DQ228_RS05630	-	1087933..1088436 (-)	504	WP_011681213.1	hypothetical protein	-
DQ228_RS05635	-	1088426..1088878 (-)	453	WP_011681214.1	hypothetical protein	-

Sequence

Protein

• Length: 293 a.a.
• Molecular weight: 31768.48 Da
• Isoelectric Point: 10.0031

>NTDB_id=300430 DQ228_RS05605 WP_084828863.1 1084007..1084888(-) (endA) [Streptococcus thermophilus strain CS20]
MAKGKTSLTNKQKRQFVTLIIAALIAVLGYLGTSNKLSPDNPIRQVASLVSGKSNKSIKSNYLTNSQSTPQEQLAETVMT
SSVKSQLGNSLEWNGAGAYIINGNKTNLNAKVSSQPYANNQVKTVQGQTVPTVANALLSKATRQYKNRQETGNGSTSWTP
AGWHQVQNLSGEYSHAVDRGHLLGYALIGGLKGFDASTSNPENIAVQTAWANQAYSDNSTGQNYYESLVRKALDKNKRVR
YRVTLIYEGENLIPSGTHLEAKSADGSLEFNVFVPNVQEGITLDYYSGKVTVN

Nucleotide

• Length: 882 bp

>NTDB_id=300430 DQ228_RS05605 WP_084828863.1 1084007..1084888(-) (endA) [Streptococcus thermophilus strain CS20]
ATGGCAAAAGGAAAAACATCTTTGACAAATAAACAGAAACGTCAGTTCGTCACATTAATAATAGCAGCTTTGATAGCTGT
TTTAGGTTACCTAGGAACCAGCAACAAGCTCTCGCCTGATAATCCTATCAGACAAGTAGCAAGCCTAGTTTCAGGAAAAT
CTAATAAATCCATTAAATCTAATTATTTAACAAATAGTCAGTCTACACCACAAGAGCAGCTTGCTGAGACAGTAATGACT
AGTTCAGTCAAAAGCCAACTGGGTAATTCCTTGGAATGGAATGGAGCTGGAGCCTATATTATTAATGGAAATAAGACGAA
TCTAAATGCTAAGGTATCAAGCCAACCTTATGCTAATAACCAAGTCAAAACGGTTCAAGGACAAACTGTTCCTACAGTTG
CTAATGCCTTATTAAGTAAAGCAACTAGACAGTACAAAAATCGTCAAGAAACAGGGAATGGTTCAACCTCTTGGACACCA
GCTGGATGGCACCAGGTTCAAAATTTATCAGGAGAGTATAGCCATGCCGTAGATAGAGGCCATTTGCTAGGTTATGCACT
GATAGGTGGTTTGAAAGGATTTGATGCCTCGACTTCAAATCCAGAGAATATTGCTGTTCAAACTGCTTGGGCAAATCAAG
CATATAGCGATAATTCTACAGGTCAAAACTATTATGAAAGCCTAGTACGAAAAGCTCTGGATAAAAACAAACGTGTTCGT
TATCGTGTGACCTTGATTTATGAAGGAGAGAACCTCATTCCATCAGGAACACATCTTGAAGCTAAGTCAGCAGACGGATC
ACTTGAGTTTAATGTCTTTGTTCCAAATGTACAAGAGGGAATAACCCTAGATTACTATTCAGGAAAAGTAACGGTGAATT
GA

Domains

Predicted by InterproScan.

• DNA/RNA non-specific endonuclease (Endonuclease_NS) (98-266)

3D structure

• AlphaFold DB: A0A0E2QH56

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
endA	Streptococcus pneumoniae Rx1	75.893	76.451	0.58
endA	Streptococcus pneumoniae D39	75.893	76.451	0.58
endA	Streptococcus pneumoniae R6	75.893	76.451	0.58
endA	Streptococcus pneumoniae TIGR4	75.893	76.451	0.58