Detailed information    

insolico Bioinformatically predicted

Overview

Name   endA   Type   Machinery gene
Locus tag   SPH_RS10300 Genome accession   NC_010380
Coordinates   1944859..1945683 (-) Length   274 a.a.
NCBI ID   WP_001036790.1    Uniprot ID   A0A0B7KWT3
Organism   Streptococcus pneumoniae Hungary19A-6     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 1939859..1950683
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SPH_RS10295 (SPH_2105) - 1943237..1944568 (-) 1332 WP_000390042.1 hemolysin family protein -
  SPH_RS10300 (SPH_2106) endA 1944859..1945683 (-) 825 WP_001036790.1 DNA-entry nuclease EndA Machinery gene
  SPH_RS10305 (SPH_2107) - 1945722..1945910 (-) 189 WP_001036519.1 DNA-directed RNA polymerase subunit beta -
  SPH_RS10310 (SPH_2108) murA 1945903..1947186 (-) 1284 WP_000358028.1 UDP-N-acetylglucosamine 1-carboxyvinyltransferase -
  SPH_RS10315 (SPH_2109) - 1947210..1947440 (-) 231 WP_000250391.1 DUF1146 family protein -
  SPH_RS10320 (SPH_2110) sepM 1947498..1948535 (-) 1038 WP_000727277.1 SepM family pheromone-processing serine protease Regulator
  SPH_RS10325 (SPH_2111) coaD 1948519..1949007 (-) 489 WP_001280746.1 pantetheine-phosphate adenylyltransferase -
  SPH_RS10330 (SPH_2112) rsmD 1948997..1949536 (-) 540 WP_000691938.1 16S rRNA (guanine(966)-N(2))-methyltransferase RsmD -
  SPH_RS10335 (SPH_2113) asnA 1949601..1950593 (-) 993 WP_000747993.1 aspartate--ammonia ligase -

Sequence

Protein


Download         Length: 274 a.a.        Molecular weight: 29949.69 Da        Isoelectric Point: 10.1700

>NTDB_id=30036 SPH_RS10300 WP_001036790.1 1944859..1945683(-) (endA) [Streptococcus pneumoniae Hungary19A-6]
MNKKTRQTLIGLLVLLLLSTGSYYIKQMQSTANSPKTKLSQKKQAPEAPSQALAESVLTDAVKSQIKGSLEWNGSGAFIV
NGNKTNLDAKVSSKPYADNKTKTVGKETVPTVANALLSKATRQYKNRKETGNGSTSWTPPGWHQVKNLKGSYTHAVDRGH
LLGYALIGGLDGFDASTSNPKNIAVQTAWANQAQAEYSTGQNYYESKVRKALDQNKRVRYRVTLYYASNEDLVPSASQIE
AKSSDGELEFNVLVPNVQKGLQLDYRTGEVTVTQ

Nucleotide


Download         Length: 825 bp        

>NTDB_id=30036 SPH_RS10300 WP_001036790.1 1944859..1945683(-) (endA) [Streptococcus pneumoniae Hungary19A-6]
ATGAACAAAAAAACAAGACAGACACTAATCGGACTGCTAGTGTTATTGCTTTTGTCTACAGGGAGCTATTATATCAAGCA
GATGCAGTCGACAGCTAATAGTCCTAAAACCAAGCTTAGTCAGAAAAAACAAGCGCCTGAAGCTCCTAGTCAAGCATTGG
CAGAGAGTGTCTTAACAGACGCAGTCAAGAGTCAAATAAAGGGGAGTCTGGAGTGGAATGGCTCAGGTGCTTTTATCGTC
AATGGTAATAAAACAAATCTAGATGCCAAGGTTTCAAGTAAGCCCTACGCTGACAATAAAACAAAGACAGTGGGCAAGGA
AACTGTTCCAACCGTAGCTAATGCCCTCTTGTCTAAGGCCACTCGTCAGTACAAGAATCGTAAAGAAACTGGGAATGGTT
CAACTTCTTGGACTCCTCCAGGTTGGCATCAGGTCAAGAATCTAAAGGGCTCTTATACCCATGCAGTCGATAGAGGTCAT
TTGTTAGGCTATGCCTTAATCGGTGGTTTGGATGGTTTTGATGCCTCAACAAGCAATCCTAAAAACATTGCTGTTCAGAC
AGCCTGGGCAAATCAGGCACAAGCCGAGTATTCGACTGGTCAAAACTACTATGAAAGCAAGGTGCGTAAAGCCTTGGACC
AAAACAAGCGTGTCCGTTACCGTGTAACCCTTTACTACGCTTCAAACGAGGATTTAGTTCCCTCAGCTTCACAGATTGAA
GCCAAGTCTTCGGATGGAGAATTGGAATTCAATGTTCTAGTTCCCAATGTTCAAAAGGGACTTCAACTGGATTACCGAAC
TGGAGAAGTAACTGTAACTCAGTAA

Domains

Predicted by InterproScan.

DNA/RNA non-specific endonuclease (Endonuclease_NS)

(75-247)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0B7KWT3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  endA Streptococcus pneumoniae Rx1

98.175

100

0.982
  endA Streptococcus pneumoniae D39

98.175

100

0.982
  endA Streptococcus pneumoniae R6

98.175

100

0.982
  endA Streptococcus pneumoniae TIGR4

98.175

100

0.982

Multiple sequence alignment