Detailed information NTG0030008

Bioinformatically predicted

Overview

• Name: comEC/celB
• Type: Machinery gene
• Locus tag: SPH_RS05110
• Genome accession: NC_010380
• Coordinates: 973746..975986 (+)
• Length: 746 a.a.
• NCBI ID: WP_000942403.1
• Uniprot ID: B1IBB4
• Organism: Streptococcus pneumoniae Hungary19A-6
• Function: ssDNA transport into the cell (predicted from homology) DNA binding and uptake

Genomic Context

Location: 968746..980986

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
SPH_RS05075 (SPH_1048)	pyrH	968836..969573 (+)	738	WP_000002997.1	UMP kinase	-
SPH_RS05080 (SPH_1049)	frr	969582..970139 (+)	558	WP_000024409.1	ribosome recycling factor	-
SPH_RS05085 (SPH_1050)	cvfB	970199..971053 (+)	855	WP_001095445.1	RNA-binding virulence regulatory protein CvfB	-
SPH_RS05090 (SPH_1051)	-	971062..971277 (+)	216	WP_001232085.1	YozE family protein	-
SPH_RS05095 (SPH_1052)	-	971363..972367 (+)	1005	WP_000658185.1	PhoH family protein	-
SPH_RS05100 (SPH_1053)	-	972475..973044 (+)	570	WP_000443751.1	GNAT family N-acetyltransferase	-
SPH_RS05105 (SPH_1054)	comEA/celA/cilE	973112..973762 (+)	651	WP_000387336.1	ComEA family DNA-binding protein	Machinery gene
SPH_RS05110 (SPH_1055)	comEC/celB	973746..975986 (+)	2241	WP_000942403.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
SPH_RS05115 (SPH_1056)	-	976165..976353 (+)	189	WP_001809102.1	hypothetical protein	-
SPH_RS05120 (SPH_1057)	-	976385..976972 (+)	588	WP_000933542.1	ABC transporter ATP-binding protein	-
SPH_RS05125 (SPH_1058)	-	976976..978157 (+)	1182	WP_000655950.1	membrane protein	-
SPH_RS05130 (SPH_1060)	infC	978464..978994 (+)	531	WP_000848180.1	translation initiation factor IF-3	-
SPH_RS05135 (SPH_1061)	rpmI	979027..979227 (+)	201	WP_001125943.1	50S ribosomal protein L35	-
SPH_RS05140 (SPH_1062)	rplT	979279..979638 (+)	360	WP_000124836.1	50S ribosomal protein L20	-
SPH_RS05145 (SPH_1063)	-	979696..980076 (+)	381	WP_000157154.1	VOC family protein	-

Sequence

Protein

• Length: 746 a.a.
• Molecular weight: 84530.99 Da
• Isoelectric Point: 9.5181

>NTDB_id=30008 SPH_RS05110 WP_000942403.1 973746..975986(+) (comEC/celB) [Streptococcus pneumoniae Hungary19A-6]
MLQWIKNFSIPLIYLSFLLLWLYYAIFSASYLALLGFVFLLVCLFIQFPWKSAGKVLIICGIFGFWFVFQNWQQSQASQN
LADSVERVRILPDTIKVNGDSLSFRGKSNGRAFQVYYKLQSEEEKEAFQALTDLHEIGLEGKLSEPEGQRNFGGFNYQAY
LKTQGIYQTLNIKTIQSLQKIGSWDIGENLSSLRRKAVVWIKTHFPDPMRNYMTGLLLGHLDTDFEEMNELYSSLGIIHL
FALSGMQVGFFMNGFKKLLLRLGLTQEKLKWLTYPFSLIYAGLTGFSASVIRSLLQKLLAQHGVKGLDNFALTVLVLFIV
MPNFFLTAGGVLSCAYAFILTMTSKEGEGLKAVTSESLVISLGILPILSFYFAEFQPWSILLTFVFSFLFDLTFLPLLSI
LFVLSFLYPVIQLNFIFEWLEGIIRLVSQVTSRPLVFGQPNAWFLILLLISLALVYDLRKNIKKLTVLCLLITGLFLLTK
HPLENEITMLDVGQGESIFLRDVTGKTILIDVGGKAESYKKIEKWQEKMTTSNAQRTLIPYLKSRGVAKIDQLILTNTDK
ENVGDLSEVTKAFHVGEILVSKDSLKQKEFVAELQTTQTKVRSMTVGENLPIFGSQLEVLSPRKMGDGGHDDTLVLYGKF
LDKQFLFTGNLEEKGEKDLLKHYPDLKVNVLKASQHGNKKSSSPAFLEKLKPELTLISVGKSNRMKLPHQETLTRLEGIN
SKVYRTDQQGAIRFKGLDSWKIESVR

Nucleotide

• Length: 2241 bp

>NTDB_id=30008 SPH_RS05110 WP_000942403.1 973746..975986(+) (comEC/celB) [Streptococcus pneumoniae Hungary19A-6]
ATGTTACAGTGGATTAAGAATTTCTCTATTCCCCTAATTTACCTGAGTTTTCTATTACTTTGGCTTTATTACGCTATTTT
CTCAGCATCTTATCTTGCTTTGTTGGGCTTTGTTTTTCTGCTAGTCTGTCTCTTTATCCAATTTCCGTGGAAATCTGCTG
GTAAAGTTCTAATAATTTGCGGAATCTTTGGATTTTGGTTTGTTTTTCAAAATTGGCAACAGAGTCAAGCGAGTCAAAAT
CTGGCGGATTCTGTTGAAAGGGTACGGATTTTGCCTGATACTATTAAGGTTAATGGTGATAGTCTATCCTTTCGTGGCAA
GTCTAACGGTCGTGCTTTCCAAGTCTATTATAAACTCCAGTCCGAGGAGGAGAAAGAAGCCTTTCAAGCTTTAACCGACC
TGCATGAGATAGGACTAGAAGGGAAGCTTTCGGAGCCAGAAGGGCAGAGAAATTTTGGTGGCTTTAATTACCAAGCCTAT
CTGAAGACTCAGGGAATTTACCAGACTCTCAATATCAAAACAATCCAGTCACTTCAAAAGATTGGCAGTTGGGATATAGG
AGAAAACTTGTCCAGTTTACGTCGAAAGGCTGTGGTTTGGATTAAGACGCACTTTCCAGACCCTATGCGCAATTACATGA
CAGGACTCTTGCTGGGACATCTGGACACCGACTTTGAGGAGATGAATGAGCTTTATTCCAGTCTAGGAATTATCCACCTC
TTTGCCCTATCTGGTATGCAGGTAGGTTTTTTCATGAATGGATTTAAGAAACTTCTCTTGCGATTGGGCTTGACCCAAGA
AAAGTTGAAATGGCTGACTTATCCCTTTTCCCTTATCTATGCGGGACTAACTGGATTTTCAGCATCGGTTATTCGCAGTC
TCTTGCAAAAGCTACTGGCTCAACATGGGGTTAAGGGCTTGGATAATTTTGCCTTGACGGTGCTTGTCCTCTTTATTGTC
ATGCCAAACTTTTTCTTGACAGCAGGAGGAGTCTTGTCCTGCGCTTATGCTTTTATCCTGACCATGACCAGCAAAGAAGG
GGAGGGGCTCAAGGCTGTTACTAGTGAAAGTCTAGTCATCTCCTTGGGCATATTGCCCATTCTATCCTTCTATTTTGCGG
AATTTCAACCTTGGTCTATCCTTTTGACCTTTGTCTTTTCCTTTCTATTTGACTTGACCTTCTTACCGCTCTTGTCTATT
TTATTTGTCCTTTCCTTTCTCTATCCAGTCATTCAGCTGAACTTTATCTTTGAATGGTTGGAGGGCATTATTCGCTTGGT
GTCACAGGTGACAAGTAGACCTCTGGTCTTTGGACAACCCAATGCATGGTTTTTAATCCTATTGTTAATTTCCTTGGCTT
TGGTCTATGATTTGAGAAAAAACATTAAAAAGCTAACGGTATTGTGCTTATTGATTACAGGGCTCTTTCTCCTGACCAAG
CATCCACTGGAAAATGAAATCACCATGCTGGATGTGGGGCAAGGAGAAAGTATTTTCCTACGGGATGTAACTGGGAAAAC
CATTCTCATAGATGTAGGTGGTAAGGCAGAATCTTATAAGAAAATCGAAAAATGGCAAGAAAAGATGACGACCAGCAATG
CCCAGCGAACCTTGATTCCCTATCTCAAAAGTCGAGGAGTAGCTAAGATTGACCAGCTAATTTTGACTAACACGGACAAG
GAGAATGTTGGAGATTTGTCAGAGGTGACCAAGGCTTTCCATGTAGGGGAGATTCTAGTATCAAAAGACAGTCTGAAACA
GAAGGAATTTGTGGCAGAACTACAGACGACTCAAACAAAGGTGCGTAGTATGACAGTAGGGGAGAACTTGCCCATTTTTG
GAAGTCAGTTAGAAGTTCTATCTCCAAGGAAAATGGGAGATGGAGGACACGATGACACCCTAGTTCTGTATGGGAAATTC
TTGGATAAGCAATTTCTCTTCACGGGAAATTTGGAGGAGAAAGGAGAGAAGGACTTGCTGAAGCACTATCCAGACTTGAA
AGTAAATGTTTTGAAAGCTAGCCAACATGGCAATAAAAAATCATCAAGTCCAGCCTTTCTAGAAAAACTCAAACCAGAGC
TTACTCTTATCTCAGTTGGAAAGAGCAATCGAATGAAACTCCCCCATCAGGAAACATTGACACGACTGGAAGGTATCAAT
AGCAAAGTTTATCGAACTGACCAGCAAGGAGCTATACGTTTTAAGGGGTTGGATAGTTGGAAAATCGAAAGTGTTCGATA
G

Domains

Predicted by InterproScan.

• Domain of unknown function (DUF4131) (DUF4131) (30-176)

• Competence protein (Competence) (216-458)

3D structure

• AlphaFold DB: B1IBB4

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEC/celB	Streptococcus pneumoniae TIGR4	98.123	100	0.981
comEC/celB	Streptococcus pneumoniae D39	97.855	100	0.979
comEC/celB	Streptococcus pneumoniae R6	97.855	100	0.979
comEC/celB	Streptococcus pneumoniae Rx1	97.855	100	0.979
comEC/celB	Streptococcus mitis NCTC 12261	91.946	99.866	0.918
comEC/celB	Streptococcus mitis SK321	91.689	100	0.917
comEC	Lactococcus lactis subsp. cremoris KW2	44.13	99.33	0.438