Detailed information NTG0299290

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: DPV67_RS18305
• Genome accession: NZ_CP030106
• Coordinates: 3755176..3756294 (-)
• Length: 372 a.a.
• NCBI ID: WP_000347036.1
• Uniprot ID: A0A009PAD6
• Organism: Acinetobacter baumannii strain DA33382
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3750176..3761294

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
DPV67_RS18270 (DPV67_18270)	-	3751005..3751853 (+)	849	WP_000336554.1	class II glutamine amidotransferase	-
DPV67_RS18275 (DPV67_18275)	-	3752106..3752561 (+)	456	WP_000782976.1	bacteriohemerythrin	-
DPV67_RS18285 (DPV67_18285)	-	3752714..3753781 (+)	1068	WP_000092403.1	hypothetical protein	-
DPV67_RS18290 (DPV67_18290)	-	3753781..3754104 (+)	324	WP_000442585.1	RnfH family protein	-
DPV67_RS18295 (DPV67_18295)	-	3754159..3754557 (-)	399	WP_001170994.1	outer membrane protein assembly factor BamE	-
DPV67_RS18300 (DPV67_18300)	fur	3754669..3755106 (+)	438	WP_001122847.1	ferric iron uptake transcriptional regulator	-
DPV67_RS18305 (DPV67_18305)	pilU	3755176..3756294 (-)	1119	WP_000347036.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
DPV67_RS18310 (DPV67_18310)	pilT	3756322..3757359 (-)	1038	WP_000355491.1	type IV pilus twitching motility protein PilT	Machinery gene
DPV67_RS18315 (DPV67_18315)	-	3757486..3758178 (+)	693	WP_001108506.1	YggS family pyridoxal phosphate-dependent enzyme	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41905.11 Da
• Isoelectric Point: 6.6944

>NTDB_id=299290 DPV67_RS18305 WP_000347036.1 3755176..3756294(-) (pilU) [Acinetobacter baumannii strain DA33382]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRKEFAETRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=299290 DPV67_RS18305 WP_000347036.1 3755176..3756294(-) (pilU) [Acinetobacter baumannii strain DA33382]
ATGGATTTTAATGACTTGCTCAACCTGATGGTTGAAAAAAAATCATCGGATCTTTTTATTACAGATGGCGTTGCGCCCTC
TATGAAGATTAACGGGCAAATTGTTCCAATTTCAAAAAACAGCCTTTCTGGGGAAGTAATTGGTCAACTATTACATTCCA
TCATGAGTGAAAAACAACGCAAAGAATTTGCAGAAACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGTGACATGCCGGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTGCAGTTACCGCCTGTACTTAAAGATTTATCGATGACCAAACGTGGCATTATTATTTTCGTAGGCGCGACAG
GTACAGGTAAATCTACTTCATTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAGGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGTTGCATCATTACTCAGCGTGAAGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACACTACGACAAGCGCCGGATGTGATCTTAATTGGTGAGATCCGTTCTCGTGAAGTCATGGACT
ACGCGATTGGCTTTGCTGAAACAGGTCACCTTGTATTAGCTACCATGCACGCCAACAACGCCAACCAAGCGCTCGACCGT
ATTATTCACTTTTTTGAAAGTGACCGTCACAGCCAGCTTTATATGGACTTGTCACTTAACTTAAAGGCTATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAGGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCACTTAAACATGCTGACTCACCGAACGATTTACG
TTTAACCATTAAACTTGCTGATGAGGGTCCTGATCAATTATCAGACGGGAAACAGCATTTAACTTTTGACCGCCAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (31-270)

3D structure

• AlphaFold DB: A0A009PAD6

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.926	100	0.772
pilU	Pseudomonas stutzeri DSM 10701	69.293	98.925	0.685
pilU	Vibrio cholerae strain A1552	53.243	99.462	0.53
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.197	93.011	0.392