Detailed information NTG0299271

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: DPV67_RS12535
• Genome accession: NZ_CP030106
• Coordinates: 2553367..2554425 (-)
• Length: 352 a.a.
• NCBI ID: WP_002014066.1
• Uniprot ID: N9LI98
• Organism: Acinetobacter baumannii strain DA33382
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2548367..2559425

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
DPV67_RS12510 (DPV67_12510)	aroK	2548642..2549211 (-)	570	WP_000643712.1	shikimate kinase AroK	-
DPV67_RS12515 (DPV67_12515)	pilQ	2549223..2551388 (-)	2166	WP_001017040.1	type IV pilus secretin PilQ family protein	Machinery gene
DPV67_RS12520 (DPV67_12520)	pilP	2551451..2551978 (-)	528	WP_000695059.1	pilus assembly protein PilP	Machinery gene
DPV67_RS12525 (DPV67_12525)	pilO	2551989..2552729 (-)	741	WP_000076101.1	type 4a pilus biogenesis protein PilO	Machinery gene
DPV67_RS12530 (DPV67_12530)	pilN	2552726..2553367 (-)	642	WP_000201229.1	PilN domain-containing protein	Machinery gene
DPV67_RS12535 (DPV67_12535)	pilM	2553367..2554425 (-)	1059	WP_002014066.1	pilus assembly protein PilM	Machinery gene
DPV67_RS12540 (DPV67_12540)	ponA	2554587..2557142 (+)	2556	WP_072292235.1	penicillin-binding protein PBP1a	-
DPV67_RS12545 (DPV67_12545)	-	2557160..2557984 (-)	825	WP_001990541.1	putative RNA methyltransferase	-
DPV67_RS12550 (DPV67_12550)	-	2557987..2558319 (-)	333	WP_000169598.1	hypothetical protein	-

Sequence

Protein

• Length: 352 a.a.
• Molecular weight: 39032.59 Da
• Isoelectric Point: 4.4618

>NTDB_id=299271 DPV67_RS12535 WP_002014066.1 2553367..2554425(-) (pilM) [Acinetobacter baumannii strain DA33382]
MLRLYRKPNKGLMGVDISSTSVKLLELSVKNGKYWVESYALMPLPENSVVEKNILNPEAVAEALERAMNLANPQTTHAAI
AVPTSTVIHKTIEMDADMSDDEREVQIRVDAEQYIPFPLDEVSLDFEVLPDRLANPNRVNVLLVATRTENVETRVEVLEL
ADLNPKLADVESYAVERAFSVFADSLPMGANTIGILDIGHTMTTLSVMQNGKIIYTREQVFGGKQLTLEIQSRYGLSLEE
ASRAKKDRSLPDDYEIEVLDPFLDAVVQQAARSLQFFFSSSQFNEIDHILLAGGNANIPGLAKLLQQKLGYRVTIANPFL
QMGFSPQVDVQKIENDASSLMVACGLALRSFD

Nucleotide

• Length: 1059 bp

>NTDB_id=299271 DPV67_RS12535 WP_002014066.1 2553367..2554425(-) (pilM) [Acinetobacter baumannii strain DA33382]
GTGCTCAGGTTATATCGTAAACCTAATAAGGGGTTAATGGGTGTCGATATTAGTTCGACTTCTGTTAAGTTGTTAGAGCT
CTCTGTCAAGAACGGTAAATATTGGGTAGAAAGCTATGCTTTGATGCCTTTACCCGAAAACAGTGTAGTTGAAAAAAATA
TCTTAAATCCAGAAGCAGTTGCAGAGGCTTTGGAACGGGCGATGAATTTAGCAAATCCCCAAACCACTCATGCTGCAATT
GCTGTTCCGACATCGACGGTTATTCATAAAACTATCGAAATGGATGCAGATATGAGCGATGACGAACGCGAAGTTCAGAT
TCGTGTAGATGCGGAGCAGTATATACCGTTCCCTTTAGATGAGGTGAGCCTTGATTTTGAGGTTTTGCCGGATCGTCTTG
CGAATCCAAATCGTGTAAATGTGCTCTTGGTCGCCACAAGAACAGAAAACGTTGAAACACGCGTTGAAGTGCTTGAATTG
GCAGATTTAAATCCTAAATTGGCTGATGTCGAAAGTTACGCGGTTGAGCGCGCTTTTAGCGTGTTTGCTGATAGCTTACC
GATGGGTGCAAATACCATAGGGATTTTAGATATCGGTCATACCATGACGACATTATCTGTCATGCAAAATGGCAAGATTA
TTTATACACGAGAGCAGGTTTTCGGCGGAAAACAACTTACGCTTGAAATTCAAAGTCGTTATGGTTTGTCTTTAGAAGAA
GCAAGCCGAGCAAAAAAAGATCGCTCTTTACCAGATGATTATGAAATTGAAGTGCTAGACCCATTTCTAGATGCGGTAGT
TCAGCAGGCGGCCCGTTCACTACAATTTTTCTTTTCTTCATCCCAATTTAACGAAATAGACCATATTTTGCTCGCTGGTG
GAAATGCGAATATTCCAGGCCTTGCTAAGCTTTTGCAGCAAAAATTGGGCTACCGTGTCACGATTGCCAACCCGTTTTTA
CAAATGGGCTTTTCTCCTCAAGTCGATGTTCAAAAAATTGAAAATGATGCTTCATCTTTAATGGTGGCATGTGGCTTGGC
TTTAAGGAGTTTTGATTAA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-351)

3D structure

• AlphaFold DB: N9LI98

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	100	100	1
comM	Acinetobacter nosocomialis M2	99.148	100	0.991
comM	Acinetobacter baylyi ADP1	81.534	100	0.815
pilM	Legionella pneumophila strain ERS1305867	42.09	100	0.423